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Literature summary extracted from

  • Vallejo-Ruiz, V.; Haque, R.; Mir, A.M.; Schwientek, T.; Mandel, U.; Cacan, R.; Delannoy, P.; Harduin-Lepers, A.
    Delineation of the minimal catalytic domain of human Galbeta1-3GalNAc alpha2,3-sialyltransferase (hST3Gal I) (2001), Biochim. Biophys. Acta, 1549, 161-173 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.3.2 expression in COS-7 cell line of various constructs deleted in the N-terminal portion of the protein sequence. The souble forms of the protein consisting of amino acids 26-340, hST3-DELTA25, and amino acids 57-340, hST3-DELTA56, are efficiently secreted and active. Further deletion of the N-terminal region gives also rise to various polypeptides that are not active within the transfected cells and not secreted in the culture medium Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.3.2 0.0137
-
 CMP-N-acetyl-beta-neuraminate truncated enzyme protein consisting of amino acids 57-340, hST3-DELTA56, pH and temperature not specified in the publication Homo sapiens
2.4.3.2 0.018
-
 beta-D-Gal-(1->3)-beta-D-GalNAc truncated enzyme protein consisting of amino acids 26-340, hST3-DELTA25, pH and temperature not specified in the publication Homo sapiens
2.4.3.2 0.021
-
 CMP-N-acetyl-beta-neuraminate truncated enzyme protein consisting of amino acids 26-340, hST3-DELTA25, pH and temperature not specified in the publication Homo sapiens
2.4.3.2 0.23
-
 beta-D-Gal-(1->3)-beta-D-GalNAc truncated enzyme protein consisting of amino acids 57-340, hST3-DELTA56, pH and temperature not specified in the publication Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.3.2 membrane Golgi membrane Homo sapiens 16020
-

Organism

EC Number Organism UniProt Comment Textmining
2.4.3.2 Homo sapiens Q11201
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.4.3.2 glycoprotein the recombinant hST3Gal I polypeptides transiently expressed in COS-7 cells are glycosylated with complex and high mannose type glycans on each of the five potential N-glycosylation sites Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.3.2 CMP-N-acetyl-beta-neuraminate + beta-D-Gal-(1->3)-beta-D-GalNAc
-
 Homo sapiens CMP + alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-beta-D-GalNAc
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.3.2 ST3Gal I
-
 Homo sapiens