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Literature summary extracted from

  • Thornburg, C.; Walter, T.; Walker, K.
    Biocatalysis of a paclitaxel analogue conversion of baccatin III to N-debenzoyl-N-(2-furoyl)paclitaxel and characterization of an amino phenylpropanoyl CoA transferase (2017), Biochemistry, 56, 5920-5930 .
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
6.2.1.25 additional information an enzyme cascade reaction using a benzoate CoA ligase (BadA), a modified nonribosomal peptide synthase (PheAT), a phenylpropanoyltransferase (BAPT), and a benzoyltransferase (NDTNBT) to produce an anticancer paclitaxel analogue and its precursor from the commercially available biosynthetic intermediate baccatin III is established. This reaction network has great potential for optimization to scale-up production and is attractive in its regioselective O- and N-acylation steps that remove protecting group manipulations used in paclitaxel analogue synthesis Taxus baccata

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.2.1.25 Mg2+ required Taxus baccata

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.2.1.25 ATP + benzoate + CoA Taxus baccata
-
 AMP + diphosphate + benzoyl-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.25 Taxus baccata
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.25 ATP + benzoate + CoA
-
 Taxus baccata AMP + diphosphate + benzoyl-CoA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.2.1.25 BadA
-
 Taxus baccata
6.2.1.25 benzoate-CoA ligase
-
 Taxus baccata

Cofactor

EC Number Cofactor Comment Organism Structure
6.2.1.25 ATP
-
 Taxus baccata