EC Number | Cloned (Comment) | Organism |
---|---|---|
1.5.1.5 | - |
Escherichia coli |
3.5.4.9 | - |
Escherichia coli |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.5.1.5 | C58Y | kcat/Km for 5,10-methenyltetrahydrofolate is 8.4fold lower than wild-type value | Escherichia coli |
1.5.1.5 | D121A | kcat/Km for 5,10-methenyltetrahydrofolate is 600fold lower than wild-type value | Escherichia coli |
1.5.1.5 | G122D | kcat/Km for 5,10-methenyltetrahydrofolate is 84fold lower than wild-type value | Escherichia coli |
1.5.1.5 | K54S | kcat/Km for 5,10-methenyltetrahydrofolate is identical to wild-type value | Escherichia coli |
1.5.1.5 | Q98K | very low activity with 5,10-methenyltetrahydrofolate | Escherichia coli |
1.5.1.5 | R191E | very low activity with 5,10-methenyltetrahydrofolate | Escherichia coli |
1.5.1.5 | Y50S | very low activity with 5,10-methenyltetrahydrofolate | Escherichia coli |
3.5.4.9 | C58Y | kcat/Km for 5,10-methenyltetrahydrofolate is 13.2fold lower than wild-type value | Escherichia coli |
3.5.4.9 | D121A | kcat/Km for 5,10-methenyltetrahydrofolate is 2348fold lower than wild-type value | Escherichia coli |
3.5.4.9 | G122D | kcat/Km for 5,10-methenyltetrahydrofolate is 283fold lower than wild-type value | Escherichia coli |
3.5.4.9 | K54S | very low activity with 5,10-methenyltetrahydrofolate | Escherichia coli |
3.5.4.9 | Q98K | very low activity with 5,10-methenyltetrahydrofolate | Escherichia coli |
3.5.4.9 | R191E | kcat/Km for 5,10-methenyltetrahydrofolate is 1.3fold higher than wild-type value | Escherichia coli |
3.5.4.9 | Y50S | very low activity with 5,10-methenyltetrahydrofolate | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.1.5 | 0.068 | - |
5,10-methenyltetrahydrofolate | pH 7.6, 30°C, mutant enzyme G122D | Escherichia coli | |
1.5.1.5 | 0.104 | - |
5,10-methenyltetrahydrofolate | pH 7.6, 30°C, mutant enzyme D121A | Escherichia coli | |
1.5.1.5 | 0.187 | - |
NADP+ | pH 7.6, 30°C, wild-type enzyme | Escherichia coli | |
1.5.1.5 | 0.205 | - |
5,10-methenyltetrahydrofolate | pH 7.6, 30°C, mutant enzyme K54S | Escherichia coli | |
1.5.1.5 | 0.233 | - |
NADP+ | pH 7.6, 30°C, mutant enzyme K54S | Escherichia coli | |
1.5.1.5 | 0.279 | - |
5,10-methenyltetrahydrofolate | pH 7.6, 30°C, wild-type enzyme | Escherichia coli | |
1.5.1.5 | 0.302 | - |
NADP+ | pH 7.6, 30°C, mutant enzyme C58Y | Escherichia coli | |
1.5.1.5 | 0.302 | - |
5,10-methenyltetrahydrofolate | pH 7.6, 30°C, mutant enzyme C58Y | Escherichia coli | |
3.5.4.9 | 0.0045 | - |
5,10-methenyltetrahydrofolate | pH 7.6, 30°C, mutant enzyme G122D | Escherichia coli | |
3.5.4.9 | 0.015 | - |
5,10-methenyltetrahydrofolate | pH 7.6, 30°C, mutant enzyme D121A | Escherichia coli | |
3.5.4.9 | 0.026 | - |
5,10-methenyltetrahydrofolate | pH 7.6, 30°C, wild-type enzyme | Escherichia coli | |
3.5.4.9 | 0.028 | - |
5,10-methenyltetrahydrofolate | pH 7.6, 30°C, mutant enzyme R191E | Escherichia coli | |
3.5.4.9 | 0.04 | - |
5,10-methenyltetrahydrofolate | pH 7.6, 30°C, mutant enzyme C58Y | Escherichia coli |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.4.9 | 5,10-methenyltetrahydrofolate + H2O | Escherichia coli | - |
10-formyltetrahydrofolate | - |
? | |
3.5.4.9 | 5,10-methenyltetrahydrofolate + H2O | Escherichia coli K12 | - |
10-formyltetrahydrofolate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.5.1.5 | Escherichia coli | P24186 | - |
- |
1.5.1.5 | Escherichia coli K12 | P24186 | - |
- |
3.5.4.9 | Escherichia coli | P24186 | - |
- |
3.5.4.9 | Escherichia coli K12 | P24186 | - |
- |
6.3.4.3 | Clostridium perfringens | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.5.1.5 | - |
Escherichia coli |
3.5.4.9 | - |
Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.1.5 | 5,10-methylenetetrahydrofolate + NADP+ | - |
Escherichia coli | 5,10-methenyltetrahydrofolate + NADPH + H+ | - |
? | |
1.5.1.5 | 5,10-methylenetetrahydrofolate + NADP+ | - |
Escherichia coli K12 | 5,10-methenyltetrahydrofolate + NADPH + H+ | - |
? | |
3.5.4.9 | 5,10-methenyltetrahydrofolate + H2O | - |
Escherichia coli | 10-formyltetrahydrofolate | - |
? | |
3.5.4.9 | 5,10-methenyltetrahydrofolate + H2O | - |
Escherichia coli K12 | 10-formyltetrahydrofolate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
6.3.4.3 | FHS | - |
Clostridium perfringens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.4.9 | 30 | - |
assay at | Escherichia coli |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.1.5 | 0.01 | - |
5,10-methenyltetrahydrofolate | pH 7.6, 30°C, mutant enzyme D121A | Escherichia coli | |
1.5.1.5 | 0.06 | - |
5,10-methenyltetrahydrofolate | pH 7.6, 30°C, mutant enzyme G122D | Escherichia coli | |
1.5.1.5 | 2.6 | - |
5,10-methenyltetrahydrofolate | pH 7.6, 30°C, mutant enzyme C58Y | Escherichia coli | |
1.5.1.5 | 2.8 | - |
NADP+ | pH 7.6, 30°C, mutant enzyme C58Y | Escherichia coli | |
1.5.1.5 | 12.3 | - |
NADP+ | pH 7.6, 30°C, mutant enzyme K54S | Escherichia coli | |
1.5.1.5 | 14.3 | - |
5,10-methenyltetrahydrofolate | pH 7.6, 30°C, mutant enzyme K54S | Escherichia coli | |
1.5.1.5 | 17.08 | - |
NADP+ | pH 7.6, 30°C, wild-type enzyme | Escherichia coli | |
1.5.1.5 | 19.9 | - |
5,10-methenyltetrahydrofolate | pH 7.6, 30°C, wild-type enzyme | Escherichia coli | |
3.5.4.9 | 0.01 | - |
5,10-methenyltetrahydrofolate | pH 7.6, 30°C, mutant enzyme D121A | Escherichia coli | |
3.5.4.9 | 0.025 | - |
5,10-methenyltetrahydrofolate | pH 7.6, 30°C, mutant enzyme G122D | Escherichia coli | |
3.5.4.9 | 4.75 | - |
5,10-methenyltetrahydrofolate | pH 7.6, 30°C, mutant enzyme C58Y | Escherichia coli | |
3.5.4.9 | 40.9 | - |
5,10-methenyltetrahydrofolate | pH 7.6, 30°C, wild-type enzyme | Escherichia coli | |
3.5.4.9 | 57.4 | - |
5,10-methenyltetrahydrofolate | pH 7.6, 30°C, mutant enzyme R191E | Escherichia coli |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.5.4.9 | 7.6 | - |
assay at | Escherichia coli |
EC Number | General Information | Comment | Organism |
---|---|---|---|
6.3.4.3 | physiological function | in Escherichia coli, the essential function of folD can be replaced by Clostridium perfringens Fhs. The Fhs-supported folD deletion strains grow well in a complex medium and require purines and glycine as supplements for growth in M9 minimal medium. The in vivo levels of N10-formyltetrahydrofolate in the folD deletion strain carrying plasmid-borne Fhs are limiting. The folD deletion strain carrying harboring Fhs on the chromosome shows a high NADP+-to-NADPH ratio and hypersensitivity to trimethoprim | Clostridium perfringens |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.1.5 | 0.096 | - |
5,10-methenyltetrahydrofolate | pH 7.6, 30°C, mutant enzyme D121A | Escherichia coli | |
1.5.1.5 | 0.88 | - |
5,10-methenyltetrahydrofolate | pH 7.6, 30°C, mutant enzyme G122D | Escherichia coli | |
1.5.1.5 | 8.6 | - |
5,10-methenyltetrahydrofolate | pH 7.6, 30°C, mutant enzyme C58Y | Escherichia coli | |
1.5.1.5 | 9.2 | - |
NADP+ | pH 7.6, 30°C, mutant enzyme C58Y | Escherichia coli | |
1.5.1.5 | 52.7 | - |
NADP+ | pH 7.6, 30°C, mutant enzyme K54S | Escherichia coli | |
1.5.1.5 | 69.8 | - |
5,10-methenyltetrahydrofolate | pH 7.6, 30°C, mutant enzyme K54S | Escherichia coli | |
1.5.1.5 | 71.3 | - |
5,10-methenyltetrahydrofolate | pH 7.6, 30°C, wild-type enzyme | Escherichia coli | |
1.5.1.5 | 91.3 | - |
NADP+ | pH 7.6, 30°C, wild-type enzyme | Escherichia coli | |
3.5.4.9 | 0.67 | - |
5,10-methenyltetrahydrofolate | pH 7.6, 30°C, mutant enzyme D121A | Escherichia coli | |
3.5.4.9 | 5.55 | - |
5,10-methenyltetrahydrofolate | pH 7.6, 30°C, mutant enzyme G122D | Escherichia coli | |
3.5.4.9 | 118.75 | - |
5,10-methenyltetrahydrofolate | pH 7.6, 30°C, mutant enzyme C58Y | Escherichia coli | |
3.5.4.9 | 1573.1 | - |
5,10-methenyltetrahydrofolate | pH 7.6, 30°C, wild-type enzyme | Escherichia coli | |
3.5.4.9 | 2050 | - |
5,10-methenyltetrahydrofolate | pH 7.6, 30°C, mutant enzyme R191E | Escherichia coli |