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Literature summary extracted from

  • Canniffe, D.P.; Chidgey, J.W.; Hunter, C.N.
    Elucidation of the preferred routes of C8-vinyl reduction in chlorophyll and bacteriochlorophyll biosynthesis (2014), Biochem. J., 462, 433-440 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.75 gene bciA Cereibacter sphaeroides

Protein Variants

EC Number Protein Variants Comment Organism
1.3.7.13 additional information generation of an enzyme BciB knockout mutant and recombinant expression of Rhodobacter sphaeroides gene bciA in the mutant Synechocystis sp. PCC 6803

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.7.13 membrane
-
 Synechocystis sp. PCC 6803 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.75 8-vinyl protochlorophyllide a + NADPH + H+ Cereibacter sphaeroides is reduced only under conditions in which this pigment accumulates as a result of perturbed formation of chlorophyllide protochlorophyllide a + NADP+
-
 ?
1.3.1.75 8-vinyl protochlorophyllide a + NADPH + H+ Cereibacter sphaeroides DSM 158 is reduced only under conditions in which this pigment accumulates as a result of perturbed formation of chlorophyllide protochlorophyllide a + NADP+
-
 ?
1.3.1.75 C8-vinyl chlorophyllide a + NADPH + H+ Cereibacter sphaeroides 8V Chlide is the preferred substrate for BciA, with high substrate specificity chlorophyllide a + NADP+
-
 ?
1.3.1.75 C8-vinyl chlorophyllide a + NADPH + H+ Cereibacter sphaeroides DSM 158 8V Chlide is the preferred substrate for BciA, with high substrate specificity chlorophyllide a + NADP+
-
 ?
1.3.7.13 3,8-divinyl chlorophyllide a + reduced ferredoxin [iron-sulfur] cluster + NADPH + H+ Synechocystis sp. PCC 6803 preferred substrate chlorophyllide a + oxidized ferredoxin [iron-sulfur] cluster + NADP+
-
 ?
1.3.7.13 3,8-divinyl protochlorophyllide a + reduced ferredoxin [iron-sulfur] cluster + NADPH + H+ Synechocystis sp. PCC 6803 is reduced only under conditions in which this pigment accumulates as a result of perturbed formation of chlorophyllide protochlorophyllide a + oxidized ferredoxin [iron-sulfur] cluster + NADP+
-
 ?
1.3.7.13 additional information Synechocystis sp. PCC 6803 although the native BciB is able to reduce the 8V group of Pchlide, the preferred substrate for this enzyme is 8V Chlide, with only a small amount of 8E Pchlide detected in the wild-type when grown under pigment accumulating conditions ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.75 Cereibacter sphaeroides Q3IXP7
-
-
1.3.1.75 Cereibacter sphaeroides DSM 158 Q3IXP7
-
-
1.3.7.13 Synechocystis sp. PCC 6803 P74473
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.75 8-vinyl protochlorophyllide a + NADPH + H+ is reduced only under conditions in which this pigment accumulates as a result of perturbed formation of chlorophyllide Cereibacter sphaeroides protochlorophyllide a + NADP+
-
 ?
1.3.1.75 8-vinyl protochlorophyllide a + NADPH + H+ is reduced only under conditions in which this pigment accumulates as a result of perturbed formation of chlorophyllide Cereibacter sphaeroides DSM 158 protochlorophyllide a + NADP+
-
 ?
1.3.1.75 C8-vinyl chlorophyllide a + NADPH + H+ 8V Chlide is the preferred substrate for BciA, with high substrate specificity Cereibacter sphaeroides chlorophyllide a + NADP+
-
 ?
1.3.1.75 C8-vinyl chlorophyllide a + NADPH + H+ preferred substrate with high substrate specificity Cereibacter sphaeroides chlorophyllide a + NADP+
-
 ?
1.3.1.75 C8-vinyl chlorophyllide a + NADPH + H+ 8V Chlide is the preferred substrate for BciA, with high substrate specificity Cereibacter sphaeroides DSM 158 chlorophyllide a + NADP+
-
 ?
1.3.1.75 C8-vinyl chlorophyllide a + NADPH + H+ preferred substrate with high substrate specificity Cereibacter sphaeroides DSM 158 chlorophyllide a + NADP+
-
 ?
1.3.7.13 3,8-divinyl chlorophyllide a + reduced ferredoxin [iron-sulfur] cluster + NADPH + H+ preferred substrate Synechocystis sp. PCC 6803 chlorophyllide a + oxidized ferredoxin [iron-sulfur] cluster + NADP+
-
 ?
1.3.7.13 3,8-divinyl protochlorophyllide a + reduced ferredoxin [iron-sulfur] cluster + NADPH + H+ is reduced only under conditions in which this pigment accumulates as a result of perturbed formation of chlorophyllide Synechocystis sp. PCC 6803 protochlorophyllide a + oxidized ferredoxin [iron-sulfur] cluster + NADP+
-
 ?
1.3.7.13 additional information although the native BciB is able to reduce the 8V group of Pchlide, the preferred substrate for this enzyme is 8V Chlide, with only a small amount of 8E Pchlide detected in the wild-type when grown under pigment accumulating conditions Synechocystis sp. PCC 6803 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.1.75 8V reductase
-
 Cereibacter sphaeroides
1.3.1.75 8VR
-
 Cereibacter sphaeroides
1.3.1.75 BciA
-
 Cereibacter sphaeroides
1.3.1.75 C8-vinyl reductase
-
 Cereibacter sphaeroides
1.3.1.75 nucleoside-diphosphate-sugar epimerase UniProt Cereibacter sphaeroides
1.3.1.75 RSP_3070
-
 Cereibacter sphaeroides
1.3.7.13 8V reductase
-
 Synechocystis sp. PCC 6803
1.3.7.13 8VR
-
 Synechocystis sp. PCC 6803
1.3.7.13 bciB
-
 Synechocystis sp. PCC 6803
1.3.7.13 C8-vinyl reductase
-
 Synechocystis sp. PCC 6803
1.3.7.13 nucleoside-diphosphate-sugar epimerase UniProt Synechocystis sp. PCC 6803
1.3.7.13 RSP_3070
-
 Synechocystis sp. PCC 6803

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.75 NADPH
-
 Cereibacter sphaeroides
1.3.7.13 ferredoxin [iron-sulfur] cluster
-
 Synechocystis sp. PCC 6803
1.3.7.13 NADPH
-
 Synechocystis sp. PCC 6803

Expression

EC Number Organism Comment Expression
1.3.1.75 Cereibacter sphaeroides generation of an enzyme BciA knockout mutant and recombinant expression of Synechocystis sp. PCC6803 gene bciB in the mutant, gene bciB is unable to restore the accumulation of pigments containing groups reduced at the C8 position additional information

General Information

EC Number General Information Comment Organism
1.3.1.75 evolution two unrelated classes of C8-vinyl reductase are known to exist, BciA and BciB Cereibacter sphaeroides
1.3.1.75 metabolism the majority of (B)Chls utilized for light-harvesting carry an ethyl group at the C8 position (8E) of the macrocycle. This group is produced by the reduction of a vinyl group (8V), catalysed by an 8V reductase, 8VR, resulting in the production of an 8E pigment. Although BciA demonstrates flexible specificity, biosynthetic heterogeneity does not occur in this organism when its preferred substrate is present, implying that, in this case, the biosynthetic pathway is linear Cereibacter sphaeroides
1.3.7.13 evolution two unrelated classes of C8-vinyl reductase are known to exist, BciA and BciB Synechocystis sp. PCC 6803
1.3.7.13 metabolism the majority of (B)Chls utilized for light-harvesting carry an ethyl group at the C8 position (8E) of the macrocycle. This group is produced by the reduction of a vinyl group (8V), catalysed by an 8V reductase, 8VR, resulting in the production of an 8E pigment Synechocystis sp. PCC 6803
1.3.7.13 additional information BciB functions as an 8VR and does not require the presence of an additional subunit Synechocystis sp. PCC 6803