Literature summary extracted from

Alonso, H.; Kleifeld, O.; Yeheskel, A.; Ong, P.C.; Liu, Y.C.; Stok, J.E.; De Voss, J.J.; Roujeinikova, A.
Structural and mechanistic insight into alkane hydroxylation by Pseudomonas putida AlkB (2014), Biochem. J., 460, 283-293 .

Crystallization (Commentary)

EC Number	Crystallization (Comment)	Organism
1.14.15.3	molecular modeling of the transmembrane domain	Pseudomonas putida

Protein Variants

EC Number	Protein Variants	Comment	Organism
1.14.15.3	K18A	complete loss of activity, mutant protein display an altered secondary structure and decreased melting temperature	Pseudomonas putida
1.14.15.3	K193A	5-20% loss of activity	Pseudomonas putida
1.14.15.3	K359A	5-20% loss of activity	Pseudomonas putida

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
1.14.15.3	1-octyne	irreversible inhibition, oxidation product hexylketene or its equivalent, covalently binds to AlkB	Pseudomonas putida

Localization

EC Number	Localization	Comment	Organism	GeneOntology No.	Textmining
1.14.15.3	membrane	-	Pseudomonas putida	16020	-

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.14.15.3	Pseudomonas putida	-	-	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.14.15.3	2 1-octyne + 2 NADH + 2 H+ + 2 O2	-	Pseudomonas putida	1-octanoic acid + 7-octynoic acid + 2 NAD+ + 2 H2O	-	?
1.14.15.3	n-octane + NADH + H+ + O2	-	Pseudomonas putida	n-octanol + NAD+ + H2O	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
1.14.15.3	AlkB	-	Pseudomonas putida
1.14.15.3	Gpo1	-	Pseudomonas putida

Temperature Stability [°C]

EC Number	Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
1.14.15.3	71.2	-	melting temperature, mutant K18A	Pseudomonas putida
1.14.15.3	74.5	-	melting temperature, wild-type	Pseudomonas putida
1.14.15.3	75.3	-	melting temperature, mutant K359A	Pseudomonas putida
1.14.15.3	78.7	-	melting temperature, mutant K193A	Pseudomonas putida

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Release 2023.1 (January 2023)