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Literature summary extracted from
- Cytochrome P450 CYP716A254 catalyzes the formation of oleanolic acid from beta-amyrin during oleanane-type triterpenoid saponins biosynthesis in Anemone flaccida (2018), Biochem. Biophys. Res. Commun., 495, 1271-1277 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.14.126 | gene CYP716A254, DNA and amino acid sequence determination and analysis, phylogenetic tree, heterologous expression in Saccharomyces cerevisiae strain WAT11 results in oxidation of beta-amyrin at the C-28 position to oleanolic acid production | Anemone flaccida |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.14.14.126 | membrane | - |
Anemone flaccida | 16020 | - |
| 1.14.14.126 | microsome | - |
Anemone flaccida | - |
- |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.14.126 | beta-amyrin + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 | Anemone flaccida | overall reaction | oleanolate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O | - |
? |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.14.126 | Anemone flaccida | I7C6E8 | collected from Tonggubao Village, Machi Town, Changyang County, Hubei Province of China in May of 2014 | - |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.14.126 | rhizome | - |
Anemone flaccida | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.14.126 | beta-amyrin + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 | overall reaction | Anemone flaccida | oleanolate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O | - |
? | |
| 1.14.14.126 | additional information | metabolite identification by mass spectrometry | Anemone flaccida | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.14.126 | CYP716A254 | - |
Anemone flaccida |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.126 | 30 | - |
assay at | Anemone flaccida |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.126 | 7.5 | - |
assay at | Anemone flaccida |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.14.126 | cytochrome P450 | - |
Anemone flaccida | |
| 1.14.14.126 | NADPH-hemoprotein reductase | A flavoprotein containing both FMN and FAD. This enzyme catalyses the transfer of electrons from NADPH, an obligatory two-electron donor, to microsomal P-450 monooxygenases, EC 1.14.14._ | Anemone flaccida |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.14.14.126 | metabolism | cytochrome P450 monooxygenase CYP716A254 is involved in oleanane-type triterpenoid saponins biosynthesis, i.e. the biosynthesis of oleanolic acid, which involves cyclization of 2,3-oxidosqualene to the oleanane-type triterpenoid skeleton, followed by a series of oxidation reactions catalyzed by cytochrome P450 monooxygenase (CYP450), Proposed biosynthetic pathway for oleanolic acid in Anemone flaccida, overview | Anemone flaccida |
| 1.14.14.126 | physiological function | cytochrome P450 monooxygenase CYP716A254 catalyzes the formation of oleanolic acid from beta-amyrin during oleanane-type triterpenoid saponins biosynthesis. Oleanolic acid is natural triterpenoid in plants with diverse biological activities | Anemone flaccida |
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