BRENDA - Enzyme Database

Allelic variants from Dahlia variabilis encode with functional differences in chalcone 3-hydroxylase activity

Schlangen, K.; Miosic, S.; Halbwirth, H.; Arch. Biochem. Biophys. 494, 40-45 (2010)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
1.14.11.B18
-
Dahlia pinnata
Engineering
EC Number
Protein Variants
Commentary
Organism
1.14.11.B18
V425A
complete loss of the ability th hydroxylate isoliquiritigenin in position 3
Dahlia pinnata
1.14.14.82
V425A
mutation in allele 2, complete loss of the ability th hydroxylate the 6'-deoxychalcone isoliquiritigenin in position 3, i.e. reaction of chalcone 3-hydroxylase
Dahlia pinnata
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.11.B18
0.0039
-
isoliquiritigenin
pH 7.5, 30°C
Dahlia pinnata
1.14.14.82
0.0005
-
naringenin
allele 2, pH 7.5, 30°C
Dahlia pinnata
1.14.14.82
0.0009
-
naringenin
allele 1, pH 7.5, 30°C
Dahlia pinnata
1.14.14.82
0.0013
-
dihydrokaempferol
allele 2, pH 7.5, 30°C
Dahlia pinnata
1.14.14.82
0.0035
-
dihydrokaempferol
allele 1, pH 7.5, 30°C
Dahlia pinnata
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.14.14.82
dihydrokaempferol + [reduced NADPH-hemoprotein reductase] + O2
Dahlia pinnata
-
dihydroquercetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
1.14.14.82
additional information
Dahlia pinnata
allele 2 additionally catalyzes the hydroxylation of the 6'-deoxychalcone isoliquiritigenin in position 3, i.e. reaction of chalcone 3-hydroxylase
?
-
-
?
1.14.14.82
naringenin + [reduced NADPH-hemoprotein reductase] + O2
Dahlia pinnata
-
eriodictyol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.14.11.B18
Dahlia pinnata
D3IWE6
D3IWE6 i.e. allele 2, enzyme additionally catalyzes the reaction of EC 1.14.14.82
-
1.14.14.82
Dahlia pinnata
D3IWE5 and D3IWE6
D3IWE5 i.e. allele 1, D3IWE6 i.e. allele 2
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.14.11.B18
isoliquiritigenin + 2-oxoglutarate + O2
hydroxylation in position 3, i.e. reaction of chalcone 3-hydroxylase
744156
Dahlia pinnata
butein + succinate + CO2
-
-
-
?
1.14.11.B18
naringenin chalcone + 2-oxoglutarate + O2
-
744156
Dahlia pinnata
eriodictyol chalcone
-
-
-
?
1.14.14.82
dihydrokaempferol + [reduced NADPH-hemoprotein reductase] + O2
-
744156
Dahlia pinnata
dihydroquercetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
1.14.14.82
additional information
allele 2 additionally catalyzes the hydroxylation of the 6'-deoxychalcone isoliquiritigenin in position 3, i.e. reaction of chalcone 3-hydroxylase
744156
Dahlia pinnata
?
-
-
-
?
1.14.14.82
additional information
allele 2 addtionally catalyzes the hydroxylation of the 6'-deoxychalcone isoliquiritigenin in position 3, i.e. reaction of chalcone 3-hydroxylase
744156
Dahlia pinnata
?
-
-
-
?
1.14.14.82
naringenin + [reduced NADPH-hemoprotein reductase] + O2
-
744156
Dahlia pinnata
eriodictyol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Synonyms
EC Number
Synonyms
Commentary
Organism
1.14.11.B18
Cyp75B
-
Dahlia pinnata
1.14.14.82
Cyp75B
-
Dahlia pinnata
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.14.11.B18
7.5
-
-
Dahlia pinnata
1.14.14.82
7.3
-
both allele 1 and 2
Dahlia pinnata
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.11.B18
-
Dahlia pinnata
Engineering (protein specific)
EC Number
Protein Variants
Commentary
Organism
1.14.11.B18
V425A
complete loss of the ability th hydroxylate isoliquiritigenin in position 3
Dahlia pinnata
1.14.14.82
V425A
mutation in allele 2, complete loss of the ability th hydroxylate the 6'-deoxychalcone isoliquiritigenin in position 3, i.e. reaction of chalcone 3-hydroxylase
Dahlia pinnata
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.11.B18
0.0039
-
isoliquiritigenin
pH 7.5, 30°C
Dahlia pinnata
1.14.14.82
0.0005
-
naringenin
allele 2, pH 7.5, 30°C
Dahlia pinnata
1.14.14.82
0.0009
-
naringenin
allele 1, pH 7.5, 30°C
Dahlia pinnata
1.14.14.82
0.0013
-
dihydrokaempferol
allele 2, pH 7.5, 30°C
Dahlia pinnata
1.14.14.82
0.0035
-
dihydrokaempferol
allele 1, pH 7.5, 30°C
Dahlia pinnata
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.14.14.82
dihydrokaempferol + [reduced NADPH-hemoprotein reductase] + O2
Dahlia pinnata
-
dihydroquercetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
1.14.14.82
additional information
Dahlia pinnata
allele 2 additionally catalyzes the hydroxylation of the 6'-deoxychalcone isoliquiritigenin in position 3, i.e. reaction of chalcone 3-hydroxylase
?
-
-
?
1.14.14.82
naringenin + [reduced NADPH-hemoprotein reductase] + O2
Dahlia pinnata
-
eriodictyol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.14.11.B18
isoliquiritigenin + 2-oxoglutarate + O2
hydroxylation in position 3, i.e. reaction of chalcone 3-hydroxylase
744156
Dahlia pinnata
butein + succinate + CO2
-
-
-
?
1.14.11.B18
naringenin chalcone + 2-oxoglutarate + O2
-
744156
Dahlia pinnata
eriodictyol chalcone
-
-
-
?
1.14.14.82
dihydrokaempferol + [reduced NADPH-hemoprotein reductase] + O2
-
744156
Dahlia pinnata
dihydroquercetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
1.14.14.82
additional information
allele 2 additionally catalyzes the hydroxylation of the 6'-deoxychalcone isoliquiritigenin in position 3, i.e. reaction of chalcone 3-hydroxylase
744156
Dahlia pinnata
?
-
-
-
?
1.14.14.82
additional information
allele 2 addtionally catalyzes the hydroxylation of the 6'-deoxychalcone isoliquiritigenin in position 3, i.e. reaction of chalcone 3-hydroxylase
744156
Dahlia pinnata
?
-
-
-
?
1.14.14.82
naringenin + [reduced NADPH-hemoprotein reductase] + O2
-
744156
Dahlia pinnata
eriodictyol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.14.11.B18
7.5
-
-
Dahlia pinnata
1.14.14.82
7.3
-
both allele 1 and 2
Dahlia pinnata