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Literature summary extracted from

  • Qin, Z.; Yan, Q.; Yang, S.; Jiang, Z.
    Modulating the function of a beta-1,3-glucanosyltransferase to that of an endo-beta-1,3-glucanase by structure-based protein engineering (2016), Appl. Microbiol. Biotechnol., 100, 1765-1776 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.39 expressed in Escherichia coli BL21(DE3) cells Rhizomucor miehei

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.39 E158A mutation switches the activity from mainly transglycosylation to beta-1,3-glucanase. Hydrolytic activity toward reduced laminarin is 348.5fold higher than the wild type Rhizomucor miehei

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.39 Rhizomucor miehei
-
-
-
3.2.1.39 Rhizomucor miehei A0A0K0M737
-
-
3.2.1.39 Rhizomucor miehei CAU432
-
-
-
3.2.1.39 Rhizomucor miehei CAU432 A0A0K0M737
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.39 Ni-IDA column chromatography, and Sephacryl S-100 gel filtration Rhizomucor miehei

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.39 0.115
-
 wild-type, beta-1,3-glucanase activity, pH 5.0, 50°C Rhizomucor miehei
3.2.1.39 2.45
-
 wild-type, transglycosylation activity, pH 5.0, 50°C Rhizomucor miehei
3.2.1.39 39.95
-
 mutant E158A, beta-1,3-glucanase activity, pH 5.0, 50°C Rhizomucor miehei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.39 2 laminaripentaose + H2O transglycosylation activity, product is a linear beta-1,3-octasaccharide containing an intrachain beta-1,6 linkage Rhizomucor miehei octasaccharide + ?
-
 ?
3.2.1.39 2 laminaripentaose + H2O transglycosylation activity, product is a linear beta-1,3-octasaccharide containing an intrachain beta-1,6 linkage Rhizomucor miehei CAU432 octasaccharide + ?
-
 ?
3.2.1.39 curdlan + H2O
-
 Rhizomucor miehei laminaribiose + laminaritriose
-
 ?
3.2.1.39 curdlan + H2O
-
 Rhizomucor miehei CAU432 laminaribiose + laminaritriose
-
 ?
3.2.1.39 laminaripentaose + H2O efficient hydrolytic activity Rhizomucor miehei laminaritetraose + ?
-
 ?
3.2.1.39 laminaripentaose + H2O efficient hydrolytic activity Rhizomucor miehei CAU432 laminaritetraose + ?
-
 ?
3.2.1.39 laminaritetraose + H2O efficient hydrolytic activity Rhizomucor miehei laminaritriose + ?
-
 ?
3.2.1.39 laminaritetraose + H2O efficient hydrolytic activity Rhizomucor miehei CAU432 laminaritriose + ?
-
 ?
3.2.1.39 additional information the enzyme hardly cleaves the beta-1,3 linkage in laminaribiose Rhizomucor miehei ?
-
 ?
3.2.1.39 additional information the enzyme hardly cleaves the beta-1,3 linkage in laminaribiose Rhizomucor miehei CAU432 ?
-
 ?
3.2.1.39 reduced laminarin + H2O
-
 Rhizomucor miehei laminarioligosaccharides
-
 ?
3.2.1.39 reduced laminarin + H2O
-
 Rhizomucor miehei CAU432 laminarioligosaccharides
-
 ?
3.2.1.39 reduced laminarin + H2O high hydrolytic activity Rhizomucor miehei laminaritriose + laminaritetraose + laminari-oligosaccharides
-
 ?
3.2.1.39 reduced laminarin + H2O high hydrolytic activity Rhizomucor miehei CAU432 laminaritriose + laminaritetraose + laminari-oligosaccharides
-
 ?
3.2.1.39 yeast beta-D-glucan + H2O
-
 Rhizomucor miehei laminaritriose + laminari-oligosaccharides
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.39 ? x * 31000, SDS-PAGE Rhizomucor miehei

Synonyms

EC Number Synonyms Comment Organism
3.2.1.39 Bgt17AE158A E158A mutant of family 17 beta-1,3-glucanosyltransferase Rhizomucor miehei
3.2.1.39 endo-beta-1,3-glucanase
-
 Rhizomucor miehei

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.39 55
-
-
 Rhizomucor miehei
3.2.1.39 55
-
 mutant E158A Rhizomucor miehei

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.39 40 60 the enzyme retains over 50 % of its activity between 40 and 60°C Rhizomucor miehei

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.39 50
-
 the enzyme is stable up to 50 °C, with more than 90 % of its activity remaining after 30 min Rhizomucor miehei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.39 4.5
-
-
 Rhizomucor miehei
3.2.1.39 4.5
-
 mutant E158A Rhizomucor miehei

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.39 4 8 the enzyme exhibits good pH stability within the range of pH 4.0-8.0, retaining over 80 % of its activity after 30 min Rhizomucor miehei