EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.1.352 | expressed in Escherichia coli | Meiothermus silvanus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.352 | 2.5 | - |
phosphate | at pH 6.0 and 42°C | Meiothermus silvanus | |
2.4.1.352 | 2.6 | - |
D-glycerate | at pH 6.5 and 42°C | Meiothermus silvanus | |
2.4.1.352 | 3.5 | - |
2-O-(alpha-D-glucopyranosyl)-D-glycerate | at pH 6.0 and 42°C | Meiothermus silvanus | |
2.4.1.352 | 8.1 | - |
alpha-D-glucopyranose 1-phosphate | at pH 6.5 and 42°C | Meiothermus silvanus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.352 | 2-O-(alpha-D-glucopyranosyl)-D-glycerate + phosphate | Meiothermus silvanus | - |
alpha-D-glucopyranose 1-phosphate + D-glycerate | - |
r | |
2.4.1.352 | 2-O-(alpha-D-glucopyranosyl)-D-glycerate + phosphate | Meiothermus silvanus ATCC 700542 | - |
alpha-D-glucopyranose 1-phosphate + D-glycerate | - |
r | |
2.4.1.352 | alpha-D-glucopyranose 1-phosphate + D-glycerate | Meiothermus silvanus | - |
2-O-(alpha-D-glucopyranosyl)-D-glycerate + phosphate | - |
r | |
2.4.1.352 | alpha-D-glucopyranose 1-phosphate + D-glycerate | Meiothermus silvanus ATCC 700542 | - |
2-O-(alpha-D-glucopyranosyl)-D-glycerate + phosphate | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.352 | Meiothermus silvanus | D7BAR0 | - |
- |
2.4.1.352 | Meiothermus silvanus ATCC 700542 | D7BAR0 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.1.352 | Ni-NTA column chromatography | Meiothermus silvanus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.352 | 2-O-(alpha-D-glucopyranosyl)-D-glycerate + phosphate | - |
Meiothermus silvanus | alpha-D-glucopyranose 1-phosphate + D-glycerate | - |
r | |
2.4.1.352 | 2-O-(alpha-D-glucopyranosyl)-D-glycerate + phosphate | - |
Meiothermus silvanus ATCC 700542 | alpha-D-glucopyranose 1-phosphate + D-glycerate | - |
r | |
2.4.1.352 | alpha-D-glucopyranose 1-phosphate + D-glycerate | - |
Meiothermus silvanus | 2-O-(alpha-D-glucopyranosyl)-D-glycerate + phosphate | - |
r | |
2.4.1.352 | alpha-D-glucopyranose 1-phosphate + D-glycerate | - |
Meiothermus silvanus ATCC 700542 | 2-O-(alpha-D-glucopyranosyl)-D-glycerate + phosphate | - |
r |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.1.352 | GGaP | - |
Meiothermus silvanus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.352 | 42 | - |
- |
Meiothermus silvanus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.352 | 0.83 | - |
2-O-(alpha-D-glucopyranosyl)-D-glycerate | at pH 6.0 and 42°C | Meiothermus silvanus | |
2.4.1.352 | 0.98 | - |
phosphate | at pH 6.0 and 42°C | Meiothermus silvanus | |
2.4.1.352 | 96 | - |
alpha-D-glucopyranose 1-phosphate | at pH 6.5 and 42°C | Meiothermus silvanus | |
2.4.1.352 | 128 | - |
D-glycerate | at pH 6.5 and 42°C | Meiothermus silvanus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.352 | 6 | - |
optimum pH in phosphorolytic direction | Meiothermus silvanus |
2.4.1.352 | 6.5 | - |
optimum pH in synthetic direction | Meiothermus silvanus |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.352 | 0.24 | - |
2-O-(alpha-D-glucopyranosyl)-D-glycerate | at pH 6.0 and 42°C | Meiothermus silvanus | |
2.4.1.352 | 0.4 | - |
phosphate | at pH 6.0 and 42°C | Meiothermus silvanus | |
2.4.1.352 | 11.8 | - |
alpha-D-glucopyranose 1-phosphate | at pH 6.5 and 42°C | Meiothermus silvanus | |
2.4.1.352 | 49.4 | - |
D-glycerate | at pH 6.5 and 42°C | Meiothermus silvanus |