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Literature summary extracted from

  • Farhan Ul-Haque, M.; Kalidass, B.; Vorobev, A.; Baral, B.S.; DiSpirito, A.A.; Semrau, J.D.
    Methanobactin from Methylocystis sp. strain SB2 affects gene expression and methane monooxygenase activity in Methylosinus trichosporium OB3b (2015), Appl. Environ. Microbiol., 81, 2466-2473 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.25 quantitative RT-PCR expression analysis of genes pmoA, mmoX, and mbnA Methylosinus trichosporium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.25 methane + NADH + H+ + O2 Methylosinus trichosporium
-
methanol + NAD+ + H2O
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.25 Methylosinus trichosporium
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.25 methane + NADH + H+ + O2
-
Methylosinus trichosporium methanol + NAD+ + H2O
-
?
1.14.13.25 additional information naphthalene assay for sMMO activity Methylosinus trichosporium ?
-
?

Synonyms

EC Number Synonyms Comment Organism
1.14.13.25 cytoplasmic methane monooxygenase
-
Methylosinus trichosporium
1.14.13.25 methane monooxygenase
-
Methylosinus trichosporium
1.14.13.25 sMMO
-
Methylosinus trichosporium
1.14.13.25 soluble methane monooxygenase
-
Methylosinus trichosporium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.13.25 30
-
assay at Methylosinus trichosporium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.13.25 7.3
-
assay at Methylosinus trichosporium

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.25 NADH
-
Methylosinus trichosporium

Expression

EC Number Organism Comment Expression
1.14.13.25 Methylosinus trichosporium the enzyme is strongly regulated by the availability of copper. Methanobactin produced by Methylosinus trichosporium OB3b plays a key role in controlling expression of MMO genes in this strain. When Methylosinus trichosporium OB3b is grown in the presence of CuCl2, expression of mmoX, encoding a subunit of the hydroxylase component of sMMO, is very low. Gene mmoX expression increases, when methanobactin from Methylocystis sp. strain SB2 (SB2-Mb) is added, as does whole-cell sMMO activity, but there is no significant change in the amount of copper associated with Methylosinus trichosporium OB3b. When Methylosinus trichosporium OB3b is grown in the absence of CuCl2, the mmoX expression level is high but decreases by several orders of magnitude when copper prebound to SB2-Mb (Cu-SB2-Mb) is added, and biomass-associated copper is increased. Exposure of Methylosinus trichosporium OB3b to SB2-Mb has no effect on expression of mbnA, encoding the polypeptide precursor of methanobactin in either the presence or absence of CuCl2. Gene mbnA expression is reduced when Cu-SB2-Mb is added in both the absence and presence of CuCl2. Methanobactin acts as a general signaling molecule in methanotrophs additional information

General Information

EC Number General Information Comment Organism
1.14.13.25 physiological function the enzyme is strongly regulated by the availability of copper. Methanobactin produced by Methylosinus trichosporium OB3b plays a key role in controlling expression of MMO genes in this strain Methylosinus trichosporium