EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.13.25 | quantitative RT-PCR expression analysis of genes pmoA, mmoX, and mbnA | Methylosinus trichosporium |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.13.25 | methane + NADH + H+ + O2 | Methylosinus trichosporium | - |
methanol + NAD+ + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.13.25 | Methylosinus trichosporium | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.13.25 | methane + NADH + H+ + O2 | - |
Methylosinus trichosporium | methanol + NAD+ + H2O | - |
? | |
1.14.13.25 | additional information | naphthalene assay for sMMO activity | Methylosinus trichosporium | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.13.25 | cytoplasmic methane monooxygenase | - |
Methylosinus trichosporium |
1.14.13.25 | methane monooxygenase | - |
Methylosinus trichosporium |
1.14.13.25 | sMMO | - |
Methylosinus trichosporium |
1.14.13.25 | soluble methane monooxygenase | - |
Methylosinus trichosporium |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.13.25 | 30 | - |
assay at | Methylosinus trichosporium |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.13.25 | 7.3 | - |
assay at | Methylosinus trichosporium |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.25 | NADH | - |
Methylosinus trichosporium |
EC Number | Organism | Comment | Expression |
---|---|---|---|
1.14.13.25 | Methylosinus trichosporium | the enzyme is strongly regulated by the availability of copper. Methanobactin produced by Methylosinus trichosporium OB3b plays a key role in controlling expression of MMO genes in this strain. When Methylosinus trichosporium OB3b is grown in the presence of CuCl2, expression of mmoX, encoding a subunit of the hydroxylase component of sMMO, is very low. Gene mmoX expression increases, when methanobactin from Methylocystis sp. strain SB2 (SB2-Mb) is added, as does whole-cell sMMO activity, but there is no significant change in the amount of copper associated with Methylosinus trichosporium OB3b. When Methylosinus trichosporium OB3b is grown in the absence of CuCl2, the mmoX expression level is high but decreases by several orders of magnitude when copper prebound to SB2-Mb (Cu-SB2-Mb) is added, and biomass-associated copper is increased. Exposure of Methylosinus trichosporium OB3b to SB2-Mb has no effect on expression of mbnA, encoding the polypeptide precursor of methanobactin in either the presence or absence of CuCl2. Gene mbnA expression is reduced when Cu-SB2-Mb is added in both the absence and presence of CuCl2. Methanobactin acts as a general signaling molecule in methanotrophs | additional information |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.14.13.25 | physiological function | the enzyme is strongly regulated by the availability of copper. Methanobactin produced by Methylosinus trichosporium OB3b plays a key role in controlling expression of MMO genes in this strain | Methylosinus trichosporium |