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Literature summary extracted from

  • Braaz, R.; Armbruster, W.; Jendrossek, D.
    Heme-dependent rubber oxygenase RoxA of Xanthomonas sp. cleaves the carbon backbone of poly(cis-1,4-Isoprene) by a dioxygenase mechanism (2005), Appl. Environ. Microbiol., 71, 2473-2478 .
    View publication on PubMedView publication on EuropePMC

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.85 Xanthomonas sp. 35Y Q7X0P3
-
-
1.13.11.87 Xanthomonas sp. 35Y Q7X0P3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.85
-
 Xanthomonas sp. 35Y
1.13.11.87
-
 Xanthomonas sp. 35Y

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.85 cis-1,4-polyisoprene + n O2
-
 Xanthomonas sp. 35Y n 12-oxo-4,8-dimethyltrideca-4,8-diene-1-al
-
 ?
1.13.11.85 poly(cis-1,4-isoprene) + O2 12-oxo-4,8-dimethyltrideca-4,8-diene-1-al is the main cleavage product in the absence of (18)O-compounds. Incorporation of one (18)O atom in 12-oxo-4,8-dimethyltrideca-4,8-diene-1-al is found if the cleavage reaction is performed in the presence of (18)O2 and H2(16)O. Incubation of poly(cis-1,4-isoprene) (with RoxA) or of isolated unlabeled 12-oxo-4,8-dimethyltrideca-4,8-diene-1-al (without RoxA) with H2(18)O in the presence of (16)O2 indicates that the carbonyl oxygen atoms of 12-oxo-4,8-dimethyltrideca-4,8-diene-1-al is significantly exchanged with oxygen atoms derived from water. The isotope exchange is avoided by simultaneous enzymatic reduction of both carbonyl functions of 12-Oxo-4,8-dimethyltrideca-4,8-diene-1-al to the corresponding dialcohol 12-hydroxy-4,8-dimethyltrideca-4,8-diene-1-ol during RoxA-mediated in vitro cleavage of poly(cis-1,4-isoprene) Xanthomonas sp. 35Y (4Z,8Z)-4,8-dimethyl-12-oxotrideca-4,8-dienal
-
 ?
1.13.11.85 rubber latex + O2 RoxA cleaves rubber by a dioxygenase mechanism Xanthomonas sp. 35Y (4Z,8Z)-4,8-dimethyl-12-oxotrideca-4,8-dienal
-
 ?
1.13.11.87 poly(cis-1,4-isoprene) + O2 12-oxo-4,8-dimethyltrideca-4,8-diene-1-al is the main cleavage product in the absence of (18)O-compounds. Incorporation of one (18)O atom in 12-oxo-4,8-dimethyltrideca-4,8-diene-1-al is found if the cleavage reaction is performed in the presence of (18)O2 and H2(16)O. Incubation of poly(cis-1,4-isoprene) (with RoxA) or of isolated unlabeled 12-oxo-4,8-dimethyltrideca-4,8-diene-1-al (without RoxA) with H2(18)O in the presence of (16)O2 indicates that the carbonyl oxygen atoms of 12-oxo-4,8-dimethyltrideca-4,8-diene-1-al is significantly exchanged with oxygen atoms derived from water. The isotope exchange is avoided by simultaneous enzymatic reduction of both carbonyl functions of 12-Oxo-4,8-dimethyltrideca-4,8-diene-1-al to the corresponding dialcohol 12-hydroxy-4,8-dimethyltrideca-4,8-diene-1-ol during RoxA-mediated in vitro cleavage of poly(cis-1,4-isoprene) Xanthomonas sp. 35Y (4Z,8Z)-4,8-dimethyl-12-oxotrideca-4,8-dienal
-
 ?
1.13.11.87 rubber latex + O2 RoxA cleaves rubber by a dioxygenase mechanism Xanthomonas sp. 35Y (4Z,8Z)-4,8-dimethyl-12-oxotrideca-4,8-dienal
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.85 roxA
-
 Xanthomonas sp. 35Y
1.13.11.87 roxA
-
 Xanthomonas sp. 35Y

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.85 40
-
 assay at Xanthomonas sp. 35Y
1.13.11.87 40
-
 assay at Xanthomonas sp. 35Y

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.85 7
-
 assay at Xanthomonas sp. 35Y
1.13.11.87 7
-
 assay at Xanthomonas sp. 35Y

Cofactor

EC Number Cofactor Comment Organism Structure
1.13.11.85 heme the enzyme contains 2 mol of heme per mol of RoxA Xanthomonas sp. 35Y
1.13.11.87 heme the enzyme contains 2 mol of heme per mol of RoxA Xanthomonas sp. 35Y