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Literature summary extracted from

  • Tian, Y.; Wang, R.; Zhao, W.; X, J.; Xing, X.; Fu, X.; Peng, R.; Yao, Q.
    Distinct properties of two glutamine synthetase isoforms in soybean root nodules (2016), Appl. Biochem. Microbiol., 52, 643-649 .
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.1.2 sequence comparisons and phylogenetic analysis, recombinant expression of His6-tagged isozyme GS1beta1 in Escherichia coli strain BL21(DE3) and in strain JW3841-1, which is Gln-auxotrophic. Isozyme GmGS1beta1 can complement the Escherichia coli Gln-auxotrophic strain JW3841-1 effectively at 37°C Glycine max
6.3.1.2 sequence comparisons and phylogenetic analysis, recombinant expression of His6-tagged isozyme GS1gamma1 in Escherichia coli strain BL21(DE3) and in strain JW3841-1, which is Gln-auxotrophic. Isozyme GmGS1gamma1 is unable to complement the Escherichia coli Gln-auxotrophic strain JW3841-1 effectively at 37°C Glycine max

Protein Variants

EC Number Protein Variants Comment Organism
6.3.1.2 additional information isozyme GmGS1beta1 can complement the Escherichia coli Gln-auxotrophic strain JW3841-1 effectively at 37°C Glycine max
6.3.1.2 additional information isozyme GmGS1gamma1 is unable to complement the Escherichia coli Gln-auxotrophic strain JW3841-1 effectively at 37°C. The lack of active GmGS1gamma1 production for the successful complementation at 37°C can be attributed to misfolding or conformational instability caused by increased temperature Glycine max

General Stability

EC Number General Stability Organism
6.3.1.2 MgCl2, MgCl2 /Glu, or MgCl2/ATP are effective in stabilizing the enzyme. Glu or ATP also stabilize the enzyme but to a lesser extent. NH4Cl accelerates the enzyme inactivation, even in the presence of MgCl2 Glycine max

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.1.2 additional information enzyme inactivation by ferric chloride reagent (0.2 M FeCl3, 0.12 M trichloroacetic acid, and 2.1% concentrated HCl). Enzyme oxidative inactivation by three different metal-catalyzed oxidation (MCO) systems, composed of air, FeCl3 and reducing agents (DTT, ascorbate or GSH), overview; enzyme inactivation by ferric chloride reagent (0.2 M FeCl3, 0.12 M trichloroacetic acid, and 2.1% concentrated HCl). Enzyme oxidative inactivation by three different metal-catalyzed oxidation (MCO) systems, composed of air, FeCl3 and reducing agents (DTT, ascorbate or GSH), overview Glycine max

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.3.1.2 cytosol
-
 Glycine max 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.1.2 Mg2+ required Glycine max

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.1.2 ATP + L-glutamate + NH3 Glycine max
-
 ADP + phosphate + L-glutamine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.1.2 Glycine max O82560
-
-
6.3.1.2 Glycine max Q9FUK4
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.1.2 root nodule
-
 Glycine max
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.1.2 ATP + L-glutamate + NH3
-
 Glycine max ADP + phosphate + L-glutamine
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.1.2 ? x * 40000, about, recombinant His6-tagged isozyme GS1beta1, SDS-PAGE, x * 39399, about, native isozyme, sequence calculation Glycine max
6.3.1.2 ? x * 40000, about, recombinant His6-tagged isozyme GS1gamma1, SDS-PAGE, x * 39399, about, native isozyme, sequence calculation Glycine max

Synonyms

EC Number Synonyms Comment Organism
6.3.1.2 glutamine synthetase cytosolic isozyme 1 UniProt Glycine max
6.3.1.2 glutamine synthetase cytosolic isozyme 2 UniProt Glycine max
6.3.1.2 GS1-2 UniProt Glycine max
6.3.1.2 GS1beta1
-
 Glycine max
6.3.1.2 GS1gamma1
-
 Glycine max

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.3.1.2 37
-
 assay at Glycine max

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.3.1.2 additional information
-
 the instability of the GmGS1gamma1 in vitro probably reflects the in vivo situation Glycine max
6.3.1.2 additional information
-
 Val161 in GS1beta1 is the key residue responsible for the heat stability Glycine max
6.3.1.2 35 60 GmGS1beta1 retains 58% of its original activity after 10 min incubation at 52°C and still about 40% of activity after 10 min at 57°C, thermal inactivation profiles of GmGS1beta1, overview Glycine max
6.3.1.2 35 60 GmGS1gamma1 only retains less than 39% of the initial activity after 10 min incubation at 52°C and is inactivated rapidly above 57°C, thermal inactivation profiles of GmGS1gamma1, overview Glycine max
6.3.1.2 50
-
 50% activity remaining Glycine max
6.3.1.2 60
-
 50% activity remaining Glycine max

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.1.2 7.5
-
 assay at Glycine max

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.1.2 ATP
-
 Glycine max

General Information

EC Number General Information Comment Organism
6.3.1.2 additional information Val161 in GS1beta1 is the key residue responsible for the heat stability Glycine max
6.3.1.2 physiological function glutamine synthetase in nodule cytosol plays a major role in the assimilation of the ammonium produced by biological nitrogen fixation Glycine max