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Literature summary extracted from

  • Rosen, B.C.; Dillon, N.A.; Peterson, N.D.; Minato, Y.; Baughn, A.D.
    Long-chain fatty acyl coenzyme A ligase FadD2 mediates intrinsic pyrazinamide resistance in Mycobacterium tuberculosis (2017), Antimicrob. Agents Chemother., 61, e02130-16 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.2.1.3 expressed in Escherichia coli BL21 cells Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.2.1.3 ATP + a long-chain fatty acid + CoA Mycobacterium tuberculosis
-
 AMP + diphosphate + a long-chain acyl-CoA
-
 ?
6.2.1.3 ATP + a long-chain fatty acid + CoA Mycobacterium tuberculosis H37Rv
-
 AMP + diphosphate + a long-chain acyl-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.3 Mycobacterium tuberculosis
-
-
-
6.2.1.3 Mycobacterium tuberculosis H37Rv
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.3 Ni-NTA agarose column chromatography Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.3 ATP + a long-chain fatty acid + CoA
-
 Mycobacterium tuberculosis AMP + diphosphate + a long-chain acyl-CoA
-
 ?
6.2.1.3 ATP + a long-chain fatty acid + CoA
-
 Mycobacterium tuberculosis H37Rv AMP + diphosphate + a long-chain acyl-CoA
-
 ?
6.2.1.3 ATP + laurate + CoA high activity Mycobacterium tuberculosis AMP + diphosphate + lauroyl-CoA
-
 ?
6.2.1.3 ATP + laurate + CoA high activity Mycobacterium tuberculosis H37Rv AMP + diphosphate + lauroyl-CoA
-
 ?
6.2.1.3 ATP + oleate + CoA high activity Mycobacterium tuberculosis AMP + diphosphate + oleoyl-CoA
-
 ?
6.2.1.3 ATP + oleate + CoA high activity Mycobacterium tuberculosis H37Rv AMP + diphosphate + oleoyl-CoA
-
 ?
6.2.1.3 ATP + palmitate + CoA highest activity Mycobacterium tuberculosis AMP + diphosphate + palmitoyl-CoA
-
 ?
6.2.1.3 ATP + palmitate + CoA highest activity Mycobacterium tuberculosis H37Rv AMP + diphosphate + palmitoyl-CoA
-
 ?
6.2.1.3 additional information the enzyme shows very low activity with acetate, propionate, butyrate, valerate, and caprylate Mycobacterium tuberculosis ?
-
 ?
6.2.1.3 additional information the enzyme shows very low activity with acetate, propionate, butyrate, valerate, and caprylate Mycobacterium tuberculosis H37Rv ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.2.1.3 FadD2
-
 Mycobacterium tuberculosis
6.2.1.3 long-chain fatty acyl coenzyme A ligase
-
 Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
6.2.1.3 ATP
-
 Mycobacterium tuberculosis

General Information

EC Number General Information Comment Organism
6.2.1.3 malfunction enzyme deletion results in enhanced susceptibility to pyrazinoic acid and rifampin but not to isoniazid or ethambutol Mycobacterium tuberculosis