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Literature summary extracted from

  • Brady, S.; Clardy, J.
    Systematic investigation of the Escherichia coli metabolome for the biosynthetic origin of an isocyanide carbon atom (2005), Angew. Chem. Int. Ed. Engl., 44, 7045-7048 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.20.12 expression in Escherichia coli uncultured organism
4.1.99.25 heterologous expression of DNA extracted directly from environmental samples (environmental DNA, eDNA) in Escherichia coli uncultured organism

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.20.12 (2S)-3-(1H-indol-3-yl)-2-isocyanopropanoate + 2-oxoglutarate + O2 uncultured organism
-
 3-[(E)-2-isocyanoethenyl]-1H-indole + succinate + 2 CO2 + H2O
-
 ?
4.1.99.25 L-tryptophan + D-ribulose 5-phosphate uncultured organism the enzyme catalyzes the biosynthesis of the isocyanide-containing antibiotic(2S)-3-(1H-indol-3-yl)-2-isocyanopropanoate (i.e. L-tryptophan isonitrile) (2S)-3-(1H-indol-3-yl)-2-isocyanopropanoate + hydroxyacetone + formaldehyde + phosphate + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.20.12 uncultured organism Q108L2
-
-
4.1.99.25 uncultured organism Q108L3
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.20.12 (2S)-3-(1H-indol-3-yl)-2-isocyanopropanoate + 2-oxoglutarate + O2
-
 uncultured organism 3-[(E)-2-isocyanoethenyl]-1H-indole + succinate + 2 CO2 + H2O
-
 ?
4.1.99.25 L-tryptophan + D-ribulose 5-phosphate the enzyme catalyzes the biosynthesis of the isocyanide-containing antibiotic(2S)-3-(1H-indol-3-yl)-2-isocyanopropanoate (i.e. L-tryptophan isonitrile) uncultured organism (2S)-3-(1H-indol-3-yl)-2-isocyanopropanoate + hydroxyacetone + formaldehyde + phosphate + H2O
-
 ?
4.1.99.25 L-tryptophan + D-ribulose 5-phosphate (2S)-3-(1H-indol-3-yl)-2-isocyanopropanoate i.e. L-tryptophan isonitrile, hydroxyacetone i.e. 1-hydroxypropan-2-one uncultured organism (2S)-3-(1H-indol-3-yl)-2-isocyanopropanoate + hydroxyacetone + formaldehyde + phosphate + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.20.12 isnB
-
 uncultured organism
4.1.99.25 isnA
-
 uncultured organism

General Information

EC Number General Information Comment Organism
1.14.20.12 physiological function presence of isonitrile synthase IsnA and IsnB alone are sufficient to generate 3-[(E)-2-isocyanoethenyl]-1H-indole from L-Trp and ribulose 5-phosphate. The origin of the isocyanide carbon atom in 3-[(E)-2-isocyanoethenyl]-1H-indole is the C2 atom of ribulose 5-phosphate, or a tautomerically equivalent sugar uncultured organism
4.1.99.25 metabolism the enzyme catalyzes the biosynthesis of the isocyanide-containing antibiotic(2S)-3-(1H-indol-3-yl)-2-isocyanopropanoate uncultured organism