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Literature summary extracted from

  • Hu, J.J.; Bai, X.L.; Liu, Y.M.; Liao, X.
    Functionalized carbon quantum dots with dopamine for tyrosinase activity analysis (2017), Anal. Chim. Acta, 995, 99-105 .
    View publication on PubMed

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.18.1 dopamine + O2 Agaricus bisporus
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Organism

EC Number Organism UniProt Comment Textmining
1.14.18.1 Agaricus bisporus
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-
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Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.18.1 commercial preparation
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 Agaricus bisporus
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.18.1 dopamine + O2
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 Agaricus bisporus ?
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1.14.18.1 dopamine + O2
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 Agaricus bisporus dopamine quinone + H2O
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 ?
1.14.18.1 additional information development of a facile fluorescent assay for TYR activity based on dopamine functionalized carbon quantum dots (CQDs-Dopa), method evaluation, overview. Dopamine (Dopa) is covalently bound to CQDs through a simple one-pot hydrothermal method, and the prepared CQDs-Dopa exhibits a fluorescence emission at 499 nm under exciting wavelength at 310 nm with a quantum yield of approximately 2.1%. When TYR is mixed with CODs-Dopa, the dopamine moiety in CQDs-Dopa conjugate is oxidized to O-dopaquinone, and an intra-particle photo-induced electron transfer process consequently occurs between CQDs and O-dopaquinone to quench the fluorescence of CQDs-Dopa. TYR activity can be determined based on the fluorescence quenching degree of CQDs-Dopa. The assay covers two broad linear ranges: 44.4-711.1 U/l and 711.1-2925.4 U/l with detection limit of 17.7 U/l. The proposed fluorescent assay is applied to TYR activity measurement in human serum samples and might be useful for TYR activity assays in clinical applications Agaricus bisporus ?
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Synonyms

EC Number Synonyms Comment Organism
1.14.18.1 mushroom tyrosine
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 Agaricus bisporus
1.14.18.1 tyr
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 Agaricus bisporus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.18.1 30
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 assay at Agaricus bisporus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.18.1 7.2
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 assay at Agaricus bisporus

General Information

EC Number General Information Comment Organism
1.14.18.1 physiological function tyrosinase (TYR) is a key enzyme in melanin biosynthesis and its activity is an important biomarker for dermatological disorders, such as vitiligo, melanoma and actinic damages Agaricus bisporus