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Literature summary extracted from

  • Sufiate, B.; Soares, F.; Moreira, S.; Gouveia, A.; Cardoso, E.; Braga, F.; de Araujo, J.; de Queiroz, J.
    In vitro and in silico characterization of a novel dextranase from Pochonia chlamydosporia (2018), 3 Biotech, 8, 167 .
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.11 molecular modeling of structure, based on PDB entry 1ogm.1 Pochonia chlamydosporia

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.11 Ag+ 10 mM, 17% residual activity Pochonia chlamydosporia
3.2.1.11 Ca2+ 10 mM, 77% residual activity Pochonia chlamydosporia
3.2.1.11 Cu2+ 10 mM, 51% residual activity Pochonia chlamydosporia
3.2.1.11 EDTA 10 mM, 80% residual activity Pochonia chlamydosporia
3.2.1.11 Fe2+ 10 mM, 71% residual activity Pochonia chlamydosporia
3.2.1.11 Hg2+ 10 mM, 17% residual activity Pochonia chlamydosporia
3.2.1.11 Mg2+ 10 mM, 60% residual activity Pochonia chlamydosporia
3.2.1.11 Mn2+ 10 mM, 89% residual activity Pochonia chlamydosporia
3.2.1.11 SDS 10 mM, 79% residual activity Pochonia chlamydosporia
3.2.1.11 Zn2+ 10 mM, 88% residual activity Pochonia chlamydosporia

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.11 additional information
-
 Dextran Km value 1.77 g/l, pH 6.0, 50°C, dextran with molecular weight from 60000 to 90000 Da Pochonia chlamydosporia

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.11 Pochonia chlamydosporia A0A179FQV9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.11
-
 Pochonia chlamydosporia

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.11 358
-
 pH 6.0, 50°C Pochonia chlamydosporia

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.11 dextran + H2O
-
 Pochonia chlamydosporia isomaltooligosaccharides
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.11 ? x * 64000, SDS-PAGE, x * 64380, calculated Pochonia chlamydosporia

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.11 50
-
-
 Pochonia chlamydosporia

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.11 60
-
 48% of maximum activity Pochonia chlamydosporia

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.11 40
-
 180 min, 48% resiudal activity Pochonia chlamydosporia
3.2.1.11 50
-
 30 min, 35% residual activity Pochonia chlamydosporia

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.11 5
-
-
 Pochonia chlamydosporia

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.11 6
-
 more than 90% of maximum activity Pochonia chlamydosporia

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.11 Pochonia chlamydosporia 74% of maximum activity
-
 4
3.2.1.11 Pochonia chlamydosporia calculated
-
 6