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Literature summary extracted from

  • Smutok, O.V.; Dmytruk, K.V.; Karkovska, M.I.; Schuhmann, W.; Gonchar, M.V.; Sibirny, A.A.
    D-Lactate-selective amperometric biosensor based on the cell debris of the recombinant yeast Hansenula polymorpha (2014), Talanta, 125, 227-232 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.2.4 gene DLD1, recombinant expression in L-lactate-cytochrome c oxidoreductase CYB2 deficient Hansenula polymorpha strain NCYC495 leu1-1 is used for recombinant overexpression of the His6-tagged enzyme using the gene with a terminator region driven by the alcohol oxidase gene promoter. The mutant strain shows increased DlDH activity and abolished activity of flavocytochrome b2, EC 1.1.2.3 Ogataea angusta

Protein Variants

EC Number Protein Variants Comment Organism
1.1.2.4 additional information construction and evaluation of amperometric cell debris-based biosensors using constant-potential amperometry in a three-electrode configuration with an Ag/AgCl/KCl(3M) reference electrode and a Pt-wire counter-electrode. The strain DELTAcyb2/DLD1 is a suitable biorecognition element for the construction of biosensors selective for D-lactate, overview Ogataea angusta

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.2.4 (R)-lactate + 2 ferricytochrome c Ogataea angusta
-
 pyruvate + 2 ferrocytochrome c
-
 ?
1.1.2.4 (R)-lactate + 2 ferricytochrome c Ogataea angusta tr6
-
 pyruvate + 2 ferrocytochrome c
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.2.4 Ogataea angusta W1QLN6
-
-
1.1.2.4 Ogataea angusta tr6 W1QLN6
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.2.4 (R)-lactate + 2 ferricytochrome c
-
 Ogataea angusta pyruvate + 2 ferrocytochrome c
-
 ?
1.1.2.4 (R)-lactate + 2 ferricytochrome c
-
 Ogataea angusta tr6 pyruvate + 2 ferrocytochrome c
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.2.4 DLD1
-
 Ogataea angusta
1.1.2.4 DLDH
-
 Ogataea angusta
1.1.2.4 L-lactate-cytochrome c oxidoreductase
-
 Ogataea angusta

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.2.4 7.8
-
 assay at Ogataea angusta

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.2.4 cytochrome c
-
 Ogataea angusta