EC Number | Cloned (Comment) | Organism |
---|---|---|
1.3.1.B14 | - |
Lyngbya majuscula |
1.3.1.104 | - |
Lyngbya majuscula |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.3.1.B14 | - |
Lyngbya majuscula |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.3.1.104 | D273A | about 55% of wild-type activity | Lyngbya majuscula |
1.3.1.104 | D273N | about 70% of wild-type activity | Lyngbya majuscula |
1.3.1.104 | K251A | activity similar to wild-type | Lyngbya majuscula |
1.3.1.104 | K251R | activity similar to wild-type | Lyngbya majuscula |
1.3.1.104 | additional information | replacement of amino acids 55-66 by CurF enoyl reductase cyclopropanase loop leads to gain-of-function activity as a cyclopropanase. With substrate 3-chloromethyl-crotonyl-[acyl-carrier-protein], the formation of both the reduced product and also the cyclopropanated productis detected at levels of about one-quarter of the total product | Lyngbya majuscula |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.1.B14 | (2E,4E,6E,10Z)-4-methyl-11-[2-[(1R,2S)-2-methylcyclopropyl]-4,5-dihydro-1,3-thiazol-4-yl]undeca-2,4,6,10-tetraenoyl-[acyl-carrier-protein] + NADPH + H+ | Lyngbya majuscula | - |
(2E,4E,6E,10Z)-4-methyl-11-[2-[(1R,2S)-2-methylcyclopropyl]-4,5-dihydro-1,3-thiazol-4-yl]undeca-4,6,10-trienoyl-[acyl-carrier-protein] + NADP+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.3.1.B14 | Lyngbya majuscula | Q6DNE2 | - |
- |
1.3.1.104 | Lyngbya majuscula | Q6E7K0 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.1.B14 | (2E,4E,6E,10Z)-4-methyl-11-[2-[(1R,2S)-2-methylcyclopropyl]-4,5-dihydro-1,3-thiazol-4-yl]undeca-2,4,6,10-tetraenoyl-[acyl-carrier-protein] + NADPH + H+ | - |
Lyngbya majuscula | (2E,4E,6E,10Z)-4-methyl-11-[2-[(1R,2S)-2-methylcyclopropyl]-4,5-dihydro-1,3-thiazol-4-yl]undeca-4,6,10-trienoyl-[acyl-carrier-protein] + NADP+ | - |
? | |
1.3.1.B14 | crotonyl-CoA + NADPH + H+ | - |
Lyngbya majuscula | butanoyl-CoA + NADP+ | - |
? | |
1.3.1.104 | crotonyl-CoA + NADPH + H+ | - |
Lyngbya majuscula | butanoyl-CoA + NADP+ | - |
? | |
1.3.1.104 | gama-chloro-beta-methylcrotonyl-[acyl-carrier-protein] + NADPH + H+ | - |
Lyngbya majuscula | gama-chloro-beta-methylbutanoyl-[acyl-carrier-protein] + NADP+ | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.3.1.B14 | curacin enoyl reductase | - |
Lyngbya majuscula |
1.3.1.B14 | CurK | - |
Lyngbya majuscula |
1.3.1.104 | JamJ | - |
Lyngbya majuscula |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.3.1.B14 | NADPH | loops that contact NADPH show flexibility | Lyngbya majuscula |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.3.1.B14 | physiological function | enzyme is involved in biosynthesis of anticancer agent curacin A | Lyngbya majuscula |