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Literature summary extracted from

  • Das, R.; Hamid, S.B.; Annuar, M.S.
    Highly efficient and stable novel nanobiohybrid catalyst to avert 3,4-dihydroxybenzoic acid pollutant in water (2016), Sci. Rep., 6, 33572 .
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
1.13.11.3 multi-walled carbon nanotube-immobilized enzyme exhibits improved thermal and pH stabilities compared to the free enzyme Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.3 0.037
-
 3,4-dihydroxybenzoate free enzyme, at pH 9.0 and 55°C Pseudomonas putida
1.13.11.3 0.095
-
 3,4-dihydroxybenzoate multi-walled carbon nanotube-immobilized enzyme, at pH 10.0 and 60°C Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.3 3,4-dihydroxybenzoate + O2 Pseudomonas putida
-
 3-carboxy-cis,cis-muconate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.3 Pseudomonas putida
-
-
-

Storage Stability

EC Number Storage Stability Organism
1.13.11.3 25°C, free enzyme, 30 days, 61% loss of activity Pseudomonas putida
1.13.11.3 25°C, immobilized enzyme, 30 days, 56% loss of activity Pseudomonas putida
1.13.11.3 4°C, free enzyme, 30 days, 59% loss of activity Pseudomonas putida
1.13.11.3 4°C, immobilized enzyme, 30 days, 56% loss of activity Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.3 3,4-dihydroxybenzoate + O2
-
 Pseudomonas putida 3-carboxy-cis,cis-muconate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.3 3,4-POD
-
 Pseudomonas putida

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.3 55
-
-
 Pseudomonas putida

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.13.11.3 90
-
 the free enzyme loses 82% of relative activity after 180 min of incubations at 90°C Pseudomonas putida

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.3 3.5
-
 3,4-dihydroxybenzoate free enzyme, at pH 9.0 and 55°C Pseudomonas putida
1.13.11.3 4.3
-
 3,4-dihydroxybenzoate multi-walled carbon nanotube-immobilized enzyme, at pH 10.0 and 60°C Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.3 9
-
-
 Pseudomonas putida

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.13.11.3 45.1
-
 3,4-dihydroxybenzoate multi-walled carbon nanotube-immobilized enzyme, at pH 10.0 and 60°C Pseudomonas putida
1.13.11.3 93.5
-
 3,4-dihydroxybenzoate free enzyme, at pH 9.0 and 55°C Pseudomonas putida