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Literature summary extracted from

  • Song, H.; Hu, W.; Naowarojna, N.; Her, A.S.; Wang, S.; Desai, R.; Qin, L.; Chen, X.; Liu, P.
    Mechanistic studies of a novel C-S lyase in ergothioneine biosynthesis the involvement of a sulfenic acid intermediate (2015), Sci. Rep., 5, 11870 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.4.1.36 expression in Escherichia coli Mycolicibacterium smegmatis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.4.1.36 0.121
-
 S-(hercyn-2-yl)-L-cysteine S-oxide pH 8.0, temperature not specified in the publication Mycolicibacterium smegmatis
4.4.1.36 1.96
-
 (2R)-3-(2-[[(2R)-2-amino-2-carboxyethyl]sulfanyl]-2,3-dihydro-1H-imidazol-4-yl)-2-(trimethylazaniumyl)propanoate pH 8.0, temperature not specified in the publication Mycolicibacterium smegmatis

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.36 Mycolicibacterium smegmatis A0R5M7
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.36 (2R)-3-(2-[[(2R)-2-amino-2-carboxyethyl]sulfanyl]-2,3-dihydro-1H-imidazol-4-yl)-2-(trimethylazaniumyl)propanoate + reduced acceptor thioether variant of S-(hercyn-2-yl)-L-cysteine S-oxide Mycolicibacterium smegmatis (2R)-3-(2-sulfido-2,3-dihydro-1H-imidazol-4-yl)-2-(trimethylazaniumyl)propanoate + pyruvate + ammonia + acceptor
-
 ?
4.4.1.36 2-(hydroxysulfanyl)hercynine + reduced acceptor
-
 Mycolicibacterium smegmatis ergothioneine + acceptor + H2O
-
 ?
4.4.1.36 additional information S-(hercyn-2-yl)-L-cysteine S-oxide is the native EgtE substrate and a sulfenic acid intermediate is involved in the ergothioneine C-S lyase reaction Mycolicibacterium smegmatis ?
-
 ?
4.4.1.36 S-(hercyn-2-yl)-L-cysteine S-oxide + H2O
-
 Mycolicibacterium smegmatis 2-(hydroxysulfanyl)hercynine + pyruvate + ammonia
-
 ?
4.4.1.36 S-(hercyn-2-yl)-L-cysteine S-oxide + reduced acceptor
-
 Mycolicibacterium smegmatis ergothioneine + pyruvate + ammonia + acceptor
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.4.1.36 egtE
-
 Mycolicibacterium smegmatis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.4.1.36 7.9
-
 (2R)-3-(2-[[(2R)-2-amino-2-carboxyethyl]sulfanyl]-2,3-dihydro-1H-imidazol-4-yl)-2-(trimethylazaniumyl)propanoate pH 8.0, temperature not specified in the publication Mycolicibacterium smegmatis
4.4.1.36 25.3
-
 S-(hercyn-2-yl)-L-cysteine S-oxide pH 8.0, temperature not specified in the publication Mycolicibacterium smegmatis

Cofactor

EC Number Cofactor Comment Organism Structure
4.4.1.36 pyridoxal 5'-phosphate 0.8 mol per mol of monomer. At pH 7.5, absorption near 420 nm, i.e. presence of ketoenamine tautomer Mycolicibacterium smegmatis