Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Chandrayan, S.K.; Wu, C.H.; McTernan, P.M.; Adams, M.W.
    High yield purification of a tagged cytoplasmic [NiFe]-hydrogenase and a catalytically-active nickel-free intermediate form (2015), Protein Expr. Purif., 107, 90-94 .
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.12.99.6 cytoplasm
-
 Pyrococcus furiosus 5737
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.12.99.6 H2 + NADPH Pyrococcus furiosus
-
 NADP + H+
-
 ?
1.12.99.6 H2 + NADPH Pyrococcus furiosus MW0430
-
 NADP + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.12.99.6 Pyrococcus furiosus
-
-
-
1.12.99.6 Pyrococcus furiosus MW0430
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.12.99.6 Ni-NTA column chromatography Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.12.99.6 H2 + NADPH
-
 Pyrococcus furiosus NADP + H+
-
 ?
1.12.99.6 H2 + NADPH
-
 Pyrococcus furiosus MW0430 NADP + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.12.99.6 heterotetramer
-
 Pyrococcus furiosus

Synonyms

EC Number Synonyms Comment Organism
1.12.99.6 SHI
-
 Pyrococcus furiosus

Cofactor

EC Number Cofactor Comment Organism Structure
1.12.99.6 FAD
-
 Pyrococcus furiosus
1.12.99.6 [2Fe-2S]-center
-
 Pyrococcus furiosus
1.12.99.6 [4Fe-4S]-center
-
 Pyrococcus furiosus