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Literature summary extracted from
- Modification of the peroxygenative peroxidative activity ratio in the unspecific peroxygenase from Agrocybe aegerita by structure-guided evolution (2017), Protein Eng. Des. Sel., 30, 191-198 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.11.2.1 | expressed in Saccharomyces cerevisiae strain BJ5465 | Cyclocybe aegerita |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.11.2.1 | F12Y/A14V/R15G/A21D/V57A/L67F/V75I/I248V/F311L | the mutant enzyme exhibits enhanced activity, secretion and stability compared to the wild type enzyme | Cyclocybe aegerita |
| 1.11.2.1 | S226G | the mutant shows increased thermal stability compared to the wild type enzyme | Cyclocybe aegerita |
| 1.11.2.1 | T120V/S226G/T320N | the mutant shows reduced activity compared to the wild type enzyme | Cyclocybe aegerita |
| 1.11.2.1 | T120V/S226G/T320R | the mutant shows reduced activity compared to the wild type enzyme | Cyclocybe aegerita |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.11.2.1 | 0.043 | - |
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant enzyme T120V/S226G/T320R, at pH 4.4 and 30°C | Cyclocybe aegerita |  |
| 1.11.2.1 | 0.043 | - |
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant enzyme T120V/S226G/T320N, at pH 4.4 and 30°C | Cyclocybe aegerita | |
| 1.11.2.1 | 0.083 | - |
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant enzyme F12Y/A14V/R15G/A21D/V57A/L67F/V75I/I248V/F311L, at pH 4.4 and 30°C | Cyclocybe aegerita | |
| 1.11.2.1 | 0.35 | - |
5-nitro-1,3-benzodioxole | mutant enzyme T120V/S226G/T320N, at pH 4.4 and 30°C | Cyclocybe aegerita | |
| 1.11.2.1 | 0.49 | - |
5-nitro-1,3-benzodioxole | mutant enzyme T120V/S226G/T320R, at pH 4.4 and 30°C | Cyclocybe aegerita | |
| 1.11.2.1 | 0.87 | - |
5-nitro-1,3-benzodioxole | mutant enzyme F12Y/A14V/R15G/A21D/V57A/L67F/V75I/I248V/F311L, at pH 4.4 and 30°C | Cyclocybe aegerita | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.11.2.1 | Cyclocybe aegerita | B9W4V6 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.11.2.1 | HiTrap SP column chromatography and BioSuite Q column chromatography | Cyclocybe aegerita |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.11.2.1 | 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2 | - |
Cyclocybe aegerita | oxidized 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O | - |
? | |
| 1.11.2.1 | 5-nitro-1,3-benzodioxole + H2O2 | - |
Cyclocybe aegerita | 4-nitrocatechol + formic acid + H2O | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.11.2.1 | UPO | - |
Cyclocybe aegerita |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.11.2.1 | 192 | - |
5-nitro-1,3-benzodioxole | mutant enzyme T120V/S226G/T320R, at pH 4.4 and 30°C | Cyclocybe aegerita | |
| 1.11.2.1 | 268 | - |
5-nitro-1,3-benzodioxole | mutant enzyme T120V/S226G/T320N, at pH 4.4 and 30°C | Cyclocybe aegerita | |
| 1.11.2.1 | 328 | - |
5-nitro-1,3-benzodioxole | mutant enzyme F12Y/A14V/R15G/A21D/V57A/L67F/V75I/I248V/F311L, at pH 4.4 and 30°C | Cyclocybe aegerita | |
| 1.11.2.1 | 343 | - |
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant enzyme T120V/S226G/T320N, at pH 4.4 and 30°C | Cyclocybe aegerita | |
| 1.11.2.1 | 380 | - |
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant enzyme T120V/S226G/T320R, at pH 4.4 and 30°C | Cyclocybe aegerita | |
| 1.11.2.1 | 952 | - |
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant enzyme F12Y/A14V/R15G/A21D/V57A/L67F/V75I/I248V/F311L, at pH 4.4 and 30°C | Cyclocybe aegerita | |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.11.2.1 | heme | - |
Cyclocybe aegerita | |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.11.2.1 | 393 | - |
5-nitro-1,3-benzodioxole | mutant enzyme T120V/S226G/T320R, at pH 4.4 and 30°C | Cyclocybe aegerita | |
| 1.11.2.1 | 406 | - |
5-nitro-1,3-benzodioxole | mutant enzyme F12Y/A14V/R15G/A21D/V57A/L67F/V75I/I248V/F311L, at pH 4.4 and 30°C | Cyclocybe aegerita | |
| 1.11.2.1 | 748 | - |
5-nitro-1,3-benzodioxole | mutant enzyme T120V/S226G/T320N, at pH 4.4 and 30°C | Cyclocybe aegerita | |
| 1.11.2.1 | 7982 | - |
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant enzyme T120V/S226G/T320N, at pH 4.4 and 30°C | Cyclocybe aegerita | |
| 1.11.2.1 | 8891 | - |
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant enzyme T120V/S226G/T320R, at pH 4.4 and 30°C | Cyclocybe aegerita | |
| 1.11.2.1 | 11424 | - |
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant enzyme F12Y/A14V/R15G/A21D/V57A/L67F/V75I/I248V/F311L, at pH 4.4 and 30°C | Cyclocybe aegerita | |
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