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Literature summary extracted from

  • Tian, F.; Greplova, M.; Frebort, I.; Dale, N.; Napier, R.
    A highly selective biosensor with nanomolar sensitivity based on cytokinin dehydrogenase (2014), PLoS ONE, 9, e90877 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.99.12 expressed in Pichia pastoris X-33 cells Zea mays

Organism

EC Number Organism UniProt Comment Textmining
1.5.99.12 Zea mays Q9T0N8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.99.12 octyl Sepharose column chromatography Zea mays

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.99.12 cis-zeatin + 2,6-dichlorophenolindophenol + H2O
-
 Zea mays ?
-
 ?
1.5.99.12 additional information adenosine, abscisic acid, gibberellic acid, and cis-zeatin riboside are no substrates, nor is the reduced dihydrozeatin. Aromatic cytokinins, kinetin and N6-benzyladenine, and the synthetic substituted urea cytokinin thidiazuron are also inactive, as are most cytokinin glucosides and the monophosphate N6-(DELTA2-isopentenyl)adenosine-5'-monophosphate Zea mays ?
-
 ?
1.5.99.12 N6-(DELTA2-isopentenyl)adenine + 2,6-dichlorophenolindophenol + H2O preferred substrate Zea mays ?
-
 ?
1.5.99.12 N6-(DELTA2-isopentenyl)adenosine + 2,6-dichlorophenolindophenol + H2O
-
 Zea mays ?
-
 ?
1.5.99.12 trans-zeatin + 2,6-dichlorophenolindophenol + H2O
-
 Zea mays ?
-
 ?
1.5.99.12 trans-zeatin riboside + 2,6-dichlorophenolindophenol + H2O most favorable substrate Zea mays ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.5.99.12 CKX1
-
 Zea mays
1.5.99.12 N6-dimethylallyladenine dehydrogenase
-
 Zea mays

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.99.12 FAD
-
 Zea mays