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Literature summary extracted from

  • Zhang, Y.J.; Wang, W.; Yang, H.L.; Li, Y.; Kang, X.Y.; Wang, X.R.; Yang, Z.L.
    Molecular Properties and functional divergence of the dehydroascorbate reductase gene family in lower and higher plants (2015), PLoS ONE, 10, e0145038 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.5.1 expressed in Escherichia coli BL21(DE3) cells Zea mays
1.8.5.1 expressed in Escherichia coli BL21(DE3) cells Pinus taeda
1.8.5.1 expressed in Escherichia coli BL21(DE3) cells Picea abies
1.8.5.1 expressed in Escherichia coli BL21(DE3) cells Populus trichocarpa
1.8.5.1 expressed in Escherichia coli BL21(DE3) cells Selaginella moellendorffii
1.8.5.1 expressed in Escherichia coli BL21(DE3) cells Brachypodium distachyon
1.8.5.1 expressed in Escherichia coli BL21(DE3) cells Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.5.1 0.068
-
dehydroascorbate isoform DHAR1, at pH 6.5 and 25°C Zea mays
1.8.5.1 0.068
-
dehydroascorbate isoform DHAR2, at pH 6.5 and 25°C Zea mays
1.8.5.1 0.113
-
dehydroascorbate isoform DHAR1, at pH 6.5 and 25°C Picea abies
1.8.5.1 0.115
-
dehydroascorbate isoform DHAR1, at pH 6.5 and 25°C Selaginella moellendorffii
1.8.5.1 0.118
-
dehydroascorbate isoform DHAR2, at pH 6.5 and 25°C Picea abies
1.8.5.1 0.119
-
dehydroascorbate isoform DHAR4, at pH 6.5 and 25°C Zea mays
1.8.5.1 0.15
-
dehydroascorbate isoform DHAR3, at pH 6.5 and 25°C Zea mays
1.8.5.1 0.248
-
dehydroascorbate isoform DHAR2, at pH 6.5 and 25°C Selaginella moellendorffii
1.8.5.1 0.74
-
glutathione isoform DHAR1, at pH 6.5 and 25°C Zea mays
1.8.5.1 1.079
-
glutathione isoform DHAR3, at pH 6.5 and 25°C Zea mays
1.8.5.1 1.344
-
glutathione isoform DHAR2, at pH 6.5 and 25°C Picea abies
1.8.5.1 1.466
-
glutathione isoform DHAR1, at pH 6.5 and 25°C Selaginella moellendorffii
1.8.5.1 2.255
-
glutathione isoform DHAR2, at pH 6.5 and 25°C Selaginella moellendorffii
1.8.5.1 2.507
-
glutathione isoform DHAR2, at pH 6.5 and 25°C Zea mays
1.8.5.1 2.705
-
glutathione isoform DHAR1, at pH 6.5 and 25°C Picea abies
1.8.5.1 2.883
-
glutathione isoform DHAR4, at pH 6.5 and 25°C Zea mays

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.8.5.1 chloroplast
-
Zea mays 9507
-
1.8.5.1 chloroplast
-
Pinus taeda 9507
-
1.8.5.1 chloroplast
-
Picea abies 9507
-
1.8.5.1 chloroplast
-
Populus trichocarpa 9507
-
1.8.5.1 chloroplast
-
Selaginella moellendorffii 9507
-
1.8.5.1 chloroplast
-
Brachypodium distachyon 9507
-
1.8.5.1 chloroplast
-
Arabidopsis thaliana 9507
-
1.8.5.1 cytoplasm
-
Zea mays 5737
-
1.8.5.1 cytoplasm
-
Pinus taeda 5737
-
1.8.5.1 cytoplasm
-
Picea abies 5737
-
1.8.5.1 cytoplasm
-
Populus trichocarpa 5737
-
1.8.5.1 cytoplasm
-
Selaginella moellendorffii 5737
-
1.8.5.1 cytoplasm
-
Brachypodium distachyon 5737
-
1.8.5.1 cytoplasm
-
Arabidopsis thaliana 5737
-
1.8.5.1 vacuole isoform DHAR3 Zea mays 5773
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.5.1 glutathione + dehydroascorbate Zea mays
-
glutathione disulfide + ascorbate
-
?
1.8.5.1 glutathione + dehydroascorbate Pinus taeda
-
glutathione disulfide + ascorbate
-
?
1.8.5.1 glutathione + dehydroascorbate Picea abies
-
glutathione disulfide + ascorbate
-
?
1.8.5.1 glutathione + dehydroascorbate Populus trichocarpa
-
glutathione disulfide + ascorbate
-
?
1.8.5.1 glutathione + dehydroascorbate Selaginella moellendorffii
-
glutathione disulfide + ascorbate
-
?
1.8.5.1 glutathione + dehydroascorbate Brachypodium distachyon
-
glutathione disulfide + ascorbate
-
?
1.8.5.1 glutathione + dehydroascorbate Arabidopsis thaliana
-
glutathione disulfide + ascorbate
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.8.5.1 Arabidopsis thaliana Q9FWR4
-
-
1.8.5.1 Brachypodium distachyon
-
-
-
1.8.5.1 Picea abies
-
-
-
1.8.5.1 Pinus taeda
-
-
-
1.8.5.1 Populus trichocarpa
-
-
-
1.8.5.1 Selaginella moellendorffii
-
-
-
1.8.5.1 Zea mays
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.5.1 Ni Sepharose column chromatography Zea mays
1.8.5.1 Ni Sepharose column chromatography Pinus taeda
1.8.5.1 Ni Sepharose column chromatography Picea abies
1.8.5.1 Ni Sepharose column chromatography Populus trichocarpa
1.8.5.1 Ni Sepharose column chromatography Selaginella moellendorffii
1.8.5.1 Ni Sepharose column chromatography Brachypodium distachyon
1.8.5.1 Ni Sepharose column chromatography Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.8.5.1 leaf
-
Zea mays
-
1.8.5.1 leaf
-
Pinus taeda
-
1.8.5.1 leaf
-
Picea abies
-
1.8.5.1 leaf
-
Populus trichocarpa
-
1.8.5.1 leaf
-
Selaginella moellendorffii
-
1.8.5.1 leaf
-
Brachypodium distachyon
-
1.8.5.1 leaf
-
Arabidopsis thaliana
-
1.8.5.1 root
-
Zea mays
-
1.8.5.1 root
-
Pinus taeda
-
1.8.5.1 root
-
Picea abies
-
1.8.5.1 root
-
Populus trichocarpa
-
1.8.5.1 root
-
Selaginella moellendorffii
-
1.8.5.1 root
-
Brachypodium distachyon
-
1.8.5.1 root
-
Arabidopsis thaliana
-
1.8.5.1 stem
-
Zea mays
-
1.8.5.1 stem
-
Pinus taeda
-
1.8.5.1 stem
-
Picea abies
-
1.8.5.1 stem
-
Populus trichocarpa
-
1.8.5.1 stem
-
Selaginella moellendorffii
-
1.8.5.1 stem
-
Brachypodium distachyon
-
1.8.5.1 stem
-
Arabidopsis thaliana
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.5.1 0.18
-
isoform DHAR2, at pH 6.5 and 25°C Picea abies
1.8.5.1 0.22
-
isoform DHAR2, at pH 6.5 and 25°C Selaginella moellendorffii
1.8.5.1 1.16
-
isoform DHAR1, at pH 6.5 and 25°C Picea abies
1.8.5.1 3.22
-
isoform DHAR4, at pH 6.5 and 25°C Zea mays
1.8.5.1 13.47
-
isoform DHAR2, at pH 6.5 and 25°C Zea mays
1.8.5.1 18.45
-
isoform DHAR1, at pH 6.5 and 25°C Zea mays
1.8.5.1 19.12
-
isoform DHAR3, at pH 6.5 and 25°C Zea mays
1.8.5.1 26.15
-
isoform DHAR1, at pH 6.5 and 25°C Selaginella moellendorffii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.5.1 glutathione + dehydroascorbate
-
Zea mays glutathione disulfide + ascorbate
-
?
1.8.5.1 glutathione + dehydroascorbate
-
Pinus taeda glutathione disulfide + ascorbate
-
?
1.8.5.1 glutathione + dehydroascorbate
-
Picea abies glutathione disulfide + ascorbate
-
?
1.8.5.1 glutathione + dehydroascorbate
-
Populus trichocarpa glutathione disulfide + ascorbate
-
?
1.8.5.1 glutathione + dehydroascorbate
-
Selaginella moellendorffii glutathione disulfide + ascorbate
-
?
1.8.5.1 glutathione + dehydroascorbate
-
Brachypodium distachyon glutathione disulfide + ascorbate
-
?
1.8.5.1 glutathione + dehydroascorbate
-
Arabidopsis thaliana glutathione disulfide + ascorbate
-
?

Synonyms

EC Number Synonyms Comment Organism
1.8.5.1 dehydroascorbate reductase
-
Zea mays
1.8.5.1 dehydroascorbate reductase
-
Pinus taeda
1.8.5.1 dehydroascorbate reductase
-
Picea abies
1.8.5.1 dehydroascorbate reductase
-
Populus trichocarpa
1.8.5.1 dehydroascorbate reductase
-
Selaginella moellendorffii
1.8.5.1 dehydroascorbate reductase
-
Brachypodium distachyon
1.8.5.1 dehydroascorbate reductase
-
Arabidopsis thaliana
1.8.5.1 DHAR1 isoform Zea mays
1.8.5.1 DHAR1 isoform Pinus taeda
1.8.5.1 DHAR1 isoform Picea abies
1.8.5.1 DHAR1 isoform Populus trichocarpa
1.8.5.1 DHAR1 isoform Selaginella moellendorffii
1.8.5.1 DHAR1 isoform Brachypodium distachyon
1.8.5.1 DHAR1 isoform Arabidopsis thaliana
1.8.5.1 DHAR2 isoform Zea mays
1.8.5.1 DHAR2 isoform Pinus taeda
1.8.5.1 DHAR2 isoform Picea abies
1.8.5.1 DHAR2 isoform Populus trichocarpa
1.8.5.1 DHAR2 isoform Selaginella moellendorffii
1.8.5.1 DHAR2 isoform Brachypodium distachyon
1.8.5.1 DHAR2 isoform Arabidopsis thaliana
1.8.5.1 DHAR3 isoform Zea mays
1.8.5.1 DHAR4 isoform Zea mays

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.8.5.1 25 50 the enzyme retains more than 45% of its maximum enzymatic activity between 25 and 50°C Zea mays
1.8.5.1 25 50 the enzyme retains more than 45% of its maximum enzymatic activity between 25 and 50°C Pinus taeda
1.8.5.1 25 50 the enzyme retains more than 45% of its maximum enzymatic activity between 25 and 50°C Picea abies
1.8.5.1 25 50 the enzyme retains more than 45% of its maximum enzymatic activity between 25 and 50°C Populus trichocarpa
1.8.5.1 25 50 the enzyme retains more than 45% of its maximum enzymatic activity between 25 and 50°C Selaginella moellendorffii
1.8.5.1 25 50 the enzyme retains more than 45% of its maximum enzymatic activity between 25 and 50°C Brachypodium distachyon
1.8.5.1 25 50 the enzyme retains more than 45% of its maximum enzymatic activity between 25 and 50°C Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.5.1 8
-
-
Zea mays
1.8.5.1 8
-
-
Pinus taeda
1.8.5.1 8
-
-
Picea abies
1.8.5.1 8
-
-
Populus trichocarpa
1.8.5.1 8
-
-
Selaginella moellendorffii
1.8.5.1 8
-
-
Brachypodium distachyon
1.8.5.1 8
-
-
Arabidopsis thaliana

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.8.5.1 5.5 10 the enzyme almost completely loses its activity below pH 5.5 or above pH 10.0 Zea mays
1.8.5.1 5.5 10 the enzyme almost completely loses its activity below pH 5.5 or above pH 10.0 Pinus taeda
1.8.5.1 5.5 10 the enzyme almost completely loses its activity below pH 5.5 or above pH 10.0 Picea abies
1.8.5.1 5.5 10 the enzyme almost completely loses its activity below pH 5.5 or above pH 10.0 Populus trichocarpa
1.8.5.1 5.5 10 the enzyme almost completely loses its activity below pH 5.5 or above pH 10.0 Selaginella moellendorffii
1.8.5.1 5.5 10 the enzyme almost completely loses its activity below pH 5.5 or above pH 10.0 Brachypodium distachyon
1.8.5.1 5.5 10 the enzyme almost completely loses its activity below pH 5.5 or above pH 10.0 Arabidopsis thaliana