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Literature summary extracted from
- Molecular and biochemical characterization of dehydroascorbate reductase from a stress adapted C4 plant, pearl millet [Pennisetum glaucum (L.) R. Br] (2014), Plant Cell Rep., 33, 435-445 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.8.5.1 | expressed in Escherichia coli Rossetta pLysS cells | Cenchrus americanus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.8.5.1 | 0.04167 | - |
glutathione | at pH 7.0 and 40°C | Cenchrus americanus |  |
| 1.8.5.1 | 0.1334 | - |
dehydroascorbate | at pH 7.0 and 40°C | Cenchrus americanus | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.5.1 | glutathione + dehydroascorbate | Cenchrus americanus | - |
glutathione disulfide + ascorbate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.8.5.1 | Cenchrus americanus | U5XYA0 | pearl millet | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.8.5.1 | Ni-NTA column chromatography | Cenchrus americanus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.8.5.1 | leaf | - |
Cenchrus americanus | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.5.1 | glutathione + dehydroascorbate | - |
Cenchrus americanus | glutathione disulfide + ascorbate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.8.5.1 | ? | x * 26000, SDS-PAGE | Cenchrus americanus |
| 1.8.5.1 | ? | x * 23400, calculated from amino acid sequence | Cenchrus americanus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.8.5.1 | dehydroascorbate reductase | - |
Cenchrus americanus |
| 1.8.5.1 | DHAR | - |
Cenchrus americanus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.8.5.1 | 40 | - |
- |
Cenchrus americanus |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.8.5.1 | 30 | 50 | more than 80% activity between 30 and 50°C | Cenchrus americanus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.8.5.1 | 7 | - |
- |
Cenchrus americanus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.8.5.1 | 6.5 | 8 | more than 50% activity between pH 6.5 and 8.0 | Cenchrus americanus |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 1.8.5.1 | Cenchrus americanus | calculated from amino acid sequence | - |
6 |
Expression
| EC Number | Organism | Comment | Expression |
|---|---|---|---|
| 1.8.5.1 | Cenchrus americanus | the enzyme expression is down-regulated under salinity stress (250 mM NaCl) | down |
| 1.8.5.1 | Cenchrus americanus | the enzyme expression is up-regulated in response to high temperature (45°C) | up |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.8.5.1 | physiological function | the enzyme plays a protective role under oxidative and other abiotic stress conditions | Cenchrus americanus |
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Release 2023.1 (January 2023)
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