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Literature summary extracted from

  • Liu, T.; Kim, D.W.; Niitsu, M.; Maeda, S.; Watanabe, M.; Kamio, Y.; Berberich, T.; Kusano, T.
    Polyamine oxidase 7 is a terminal catabolism-type enzyme in Oryza sativa and is specifically expressed in anthers (2014), Plant Cell Physiol., 55, 1110-1122 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.3.14 expression in Escherichia coli Oryza sativa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.3.14 0.0383
-
 spermine pH 6.5, 30°C Oryza sativa
1.5.3.14 0.0407
-
 spermine pH 7.0, 37°C Oryza sativa
1.5.3.14 0.0422
-
 spermidine pH 7.0, 37°C Oryza sativa
1.5.3.14 0.045
-
 spermidine pH 6.5, 30°C Oryza sativa
1.5.3.14 0.0594
-
 N1-acetylspermine pH 6.5, 30°C Oryza sativa
1.5.3.14 0.0701
-
 N1-acetylspermine pH 7.0, 37°C Oryza sativa
1.5.3.14 0.075
-
 thermospermine pH 6.5, 30°C Oryza sativa
1.5.3.14 0.0788
-
 norspermine pH 6.5, 30°C Oryza sativa
1.5.3.14 0.0803
-
 N1-acetylspermidine pH 7.0, 37°C Oryza sativa
1.5.3.14 0.081
-
 N1-acetylspermidine pH 6.5, 30°C Oryza sativa
1.5.3.14 0.0814
-
 thermospermine pH 7.0, 37°C Oryza sativa
1.5.3.14 0.0829
-
 norspermine pH 7.0, 37°C Oryza sativa
1.5.3.14 0.109
-
 norspermidine pH 7.0, 37°C Oryza sativa
1.5.3.14 0.1578
-
 norspermidine pH 6.5, 30°C Oryza sativa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.5.3.14 apoplast OsPAO7 seems to remain in a peripheral layer of the plant cell with the aid of its predicted signal peptide and transmembrane domain Oryza sativa 48046
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.3.14 64000
-
 gel filtration Oryza sativa

Organism

EC Number Organism UniProt Comment Textmining
1.5.3.14 Oryza sativa Q0J290
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.3.14
-
 Oryza sativa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.3.14 anther the enzyme is specifically expressed in anthers, with an expressional peak at the bicellular pollen stage Oryza sativa
-
1.5.3.14 pollen the enzyme is specifically expressed in anthers, with an expressional peak at the bicellular pollen stage Oryza sativa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.3.14 additional information no activity with the diamines putrescine and cadaverine Oryza sativa ?
-
 ?
1.5.3.14 N1-acetylspermidine + O2 + H2O at pH 7.0, 37°C, spermine and spermidine are the preferred substrates for OsPAO7, followed by N1-acetylspermine, N1-acetylspermidine, norspermine, thermospermine and norspermidine. At pH 6.5, 30°C, OsPAO7 shows a similar polyamine preference; it favors spermine and spermidine, followed by N1-acetylspermine, norspermine, thermospermine, N1-acetylspermidine and norspermidine Oryza sativa 1,3-diaminopropane + H2O2 + ?
-
 ?
1.5.3.14 N1-acetylspermine + O2 + H2O at pH 7.0, 37°C, spermine and spermidine are the preferred substrates for OsPAO7, followed by N1-acetylspermine, N1-acetylspermidine, norspermine, thermospermine and norspermidine. At pH 6.5, 30°C, OsPAO7 shows a similar polyamine preference; it favors spermine and spermidine, followed by N1-acetylspermine, norspermine, thermospermine, N1-acetylspermidine and norspermidine Oryza sativa 1,3-diaminopropane + H2O2 + ?
-
 ?
1.5.3.14 norspermidine + O2 + H2O at pH 7.0, 37°C, spermine and spermidine are the preferred substrates for OsPAO7, followed by N1-acetylspermine, N1-acetylspermidine, norspermine, thermospermine and norspermidine. At pH 6.5, 30°C, OsPAO7 shows a similar polyamine preference; it favors spermine and spermidine, followed by N1-acetylspermine, norspermine, thermospermine, N1-acetylspermidine and norspermidine Oryza sativa 1,3-diaminopropane + H2O2 + ?
-
 ?
1.5.3.14 norspermine + O2 + H2O at pH 7.0, 37°C, spermine and spermidine are the preferred substrates for OsPAO7, followed by N1-acetylspermine, N1-acetylspermidine, norspermine, thermospermine and norspermidine. At pH 6.5, 30°C, OsPAO7 shows a similar polyamine preference; it favors spermine and spermidine, followed by N1-acetylspermine, norspermine, thermospermine, N1-acetylspermidine and norspermidine Oryza sativa ?
-
 ?
1.5.3.14 spermidine + O2 + H2O at pH 7.0, 37°C, spermine and spermidine are the preferred substrates for OsPAO7, followed by N1-acetylspermine, N1-acetylspermidine, norspermine, thermospermine and norspermidine. At pH 6.5, 30°C, OsPAO7 shows a similar polyamine preference; it favors spermine and spermidine, followed by N1-acetylspermine, norspermine, thermospermine, N1-acetylspermidine and norspermidine Oryza sativa 4-aminobutanal + propane-1,3-diamine + H2O2
-
 ?
1.5.3.14 spermine + O2 + H2O at pH 7.0, 37°C, spermine and spermidine are the preferred substrates for OsPAO7, followed by N1-acetylspermine, N1-acetylspermidine, norspermine, thermospermine and norspermidine. At pH 6.5, 30°C, OsPAO7 shows a similar polyamine preference; it favors spermine and spermidine, followed by N1-acetylspermine, norspermine, thermospermine, N1-acetylspermidine and norspermidine Oryza sativa N-(3-aminopropyl)-4-aminobutanal + 1,3-diaminopropane + H2O2
-
 ?
1.5.3.14 thermospermine + O2 + H2O at pH 7.0, 37°C, spermine and spermidine are the preferred substrates for OsPAO7, followed by N1-acetylspermine, N1-acetylspermidine, norspermine, thermospermine and norspermidine. At pH 6.5, 30°C, OsPAO7 shows a similar polyamine preference; it favors spermine and spermidine, followed by N1-acetylspermine, norspermine, thermospermine, N1-acetylspermidine and norspermidine Oryza sativa 1,3-diaminopropane + H2O2 + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.3.14 monomer 1 * 55000, SDS-PAGE Oryza sativa
1.5.3.14 monomer 1 * 53200, calculated from sequence Oryza sativa

Synonyms

EC Number Synonyms Comment Organism
1.5.3.14 OsPAO7
-
 Oryza sativa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.3.14 30
-
 substrate: spermine Oryza sativa
1.5.3.14 37
-
 substrate: spermidine Oryza sativa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5.3.14 1.1
-
 norspermidine pH 6.5, 30°C Oryza sativa
1.5.3.14 2.3
-
 norspermidine pH 7.0, 37°C Oryza sativa
1.5.3.14 4.5
-
 thermospermine pH 7.0, 37°C Oryza sativa
1.5.3.14 6.6
-
 N1-acetylspermidine pH 6.5, 30°C Oryza sativa
1.5.3.14 7.8
-
 thermospermine pH 6.5, 30°C Oryza sativa
1.5.3.14 8.3
-
 norspermine pH 7.0, 37°C Oryza sativa
1.5.3.14 9
-
 norspermine pH 6.5, 30°C Oryza sativa
1.5.3.14 9.8
-
 N1-acetylspermidine pH 7.0, 37°C Oryza sativa
1.5.3.14 18.2
-
 N1-acetylspermine pH 7.0, 37°C Oryza sativa
1.5.3.14 20.5
-
 N1-acetylspermine pH 6.5, 30°C Oryza sativa
1.5.3.14 26.5
-
 spermidine pH 6.5, 30°C Oryza sativa
1.5.3.14 30.6
-
 spermine pH 7.0, 37°C Oryza sativa
1.5.3.14 32.6
-
 spermine pH 6.5, 30°C Oryza sativa
1.5.3.14 287.9
-
 spermidine pH 7.0, 37°C Oryza sativa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.3.14 6.5
-
 substrate: spermine Oryza sativa
1.5.3.14 7
-
 substrate: spermidine Oryza sativa

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.3.14 FAD
-
 Oryza sativa

General Information

EC Number General Information Comment Organism
1.5.3.14 physiological function a likely role for OsPAO7 during anther development is to deliver H2O2 for secondary wall thickening through lignin formation Oryza sativa

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.5.3.14 6.97
-
 norspermidine pH 6.5, 30°C Oryza sativa
1.5.3.14 21.7
-
 norspermidine pH 7.0, 37°C Oryza sativa
1.5.3.14 55.3
-
 thermospermine pH 7.0, 37°C Oryza sativa
1.5.3.14 82.7
-
 N1-acetylspermidine pH 6.5, 30°C Oryza sativa
1.5.3.14 96.9
-
 norspermine pH 7.0, 37°C Oryza sativa
1.5.3.14 104
-
 thermospermine pH 6.5, 30°C Oryza sativa
1.5.3.14 115.1
-
 norspermine pH 6.5, 30°C Oryza sativa
1.5.3.14 117.8
-
 N1-acetylspermidine pH 7.0, 37°C Oryza sativa
1.5.3.14 259.7
-
 N1-acetylspermine pH 7.0, 37°C Oryza sativa
1.5.3.14 345.1
-
 N1-acetylspermine pH 6.5, 30°C Oryza sativa
1.5.3.14 591.7
-
 spermidine pH 6.5, 30°C Oryza sativa
1.5.3.14 660.9
-
 spermidine pH 7.0, 37°C Oryza sativa
1.5.3.14 752.9
-
 spermine pH 7.0, 37°C Oryza sativa
1.5.3.14 853.9
-
 spermine pH 6.5, 30°C Oryza sativa