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Literature summary extracted from

  • Boettcher, C.; Westphal, L.; Schmotz, C.; Prade, E.; Scheel, D.; Glawischnig, E.
    The multifunctional enzyme CYP71B15 (phytoalexin deficient3) converts cysteine-indole-3-acetonitrile to camalexin in the indole-3-acetonitrile metabolic network of Arabidopsis thaliana (2009), Plant Cell, 21, 1830-1845 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.19.52 expressed in Saccharomyces cerevisiae strain WAT11 Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.19.52 0.0292
-
 2-(L-cystein-S-yl)-2-(1H-indol-3-yl)acetonitrile pH and temperature not specified in the publication Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.19.52 2-(L-cystein-S-yl)-2-(1H-indol-3-yl)acetonitrile + [reduced NADPH-hemoprotein reductase] + O2 Arabidopsis thaliana
-
 camalexin + hydrogen cyanide + CO2 + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.19.52 Arabidopsis thaliana Q9LW27
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.19.52 leaf
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.19.52 2-(L-cystein-S-yl)-2-(1H-indol-3-yl)acetonitrile + [reduced NADPH-hemoprotein reductase] + O2
-
 Arabidopsis thaliana camalexin + hydrogen cyanide + CO2 + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.19.52 CYP71B15
-
 Arabidopsis thaliana
1.14.19.52 PAD3 phytoalexin deficient3 Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.19.52 NADPH
-
 Arabidopsis thaliana