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Literature summary extracted from

  • Gisin, J.; Schneider, A.; Naegele, B.; Borisova, M.; Mayer, C.
    A cell wall recycling shortcut that bypasses peptidoglycan de novo biosynthesis (2013), Nat. Chem. Biol., 9, 491-493 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.221 expression in Escherichia coli Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.221 0.0508
-
 ATP pH not specified in the publication, temperature not specified in the publication Pseudomonas putida
2.7.1.221 0.08
-
 N-acetyl-D-muramate pH not specified in the publication, temperature not specified in the publication Pseudomonas putida
2.7.1.221 0.177
-
 N-acetyl-D-glucosamine pH not specified in the publication, temperature not specified in the publication Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.221 ATP + N-acetyl-D-galactosamine Pseudomonas putida
-
 ADP + N-acetyl-D-galactosamine 1-phosphate
-
 ?
2.7.1.221 ATP + N-acetyl-D-galactosamine Pseudomonas putida DSM 6125
-
 ADP + N-acetyl-D-galactosamine 1-phosphate
-
 ?
2.7.1.221 ATP + N-acetyl-D-glucosamine Pseudomonas putida
-
 ADP + N-acetyl-D-glucosamine 1-phosphate
-
 ?
2.7.1.221 ATP + N-acetyl-D-glucosamine Pseudomonas putida DSM 6125
-
 ADP + N-acetyl-D-glucosamine 1-phosphate
-
 ?
2.7.1.221 ATP + N-acetyl-D-muramate Pseudomonas putida
-
 ADP + N-acetyl-alpha-D-muramate 1-phosphate
-
 ?
2.7.1.221 ATP + N-acetyl-D-muramate Pseudomonas putida DSM 6125
-
 ADP + N-acetyl-alpha-D-muramate 1-phosphate
-
 ?
2.7.1.221 additional information Pseudomonas putida no substrates: N-acetyl-D-mannosamine, N-deacetylated sugars or glucosamine ?
-
 ?
2.7.1.221 additional information Pseudomonas putida DSM 6125 no substrates: N-acetyl-D-mannosamine, N-deacetylated sugars or glucosamine ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.221 Pseudomonas putida Q88QT3 member of protein family PF01636
-
2.7.1.221 Pseudomonas putida DSM 6125 Q88QT3 member of protein family PF01636
-
2.7.7.99 Pseudomonas putida Q88QT2
-
-
2.7.7.99 Pseudomonas putida DSM 6125 Q88QT2
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.221 ATP + N-acetyl-D-galactosamine
-
 Pseudomonas putida ADP + N-acetyl-D-galactosamine 1-phosphate
-
 ?
2.7.1.221 ATP + N-acetyl-D-galactosamine
-
 Pseudomonas putida DSM 6125 ADP + N-acetyl-D-galactosamine 1-phosphate
-
 ?
2.7.1.221 ATP + N-acetyl-D-glucosamine
-
 Pseudomonas putida ADP + N-acetyl-D-glucosamine 1-phosphate
-
 ?
2.7.1.221 ATP + N-acetyl-D-glucosamine
-
 Pseudomonas putida DSM 6125 ADP + N-acetyl-D-glucosamine 1-phosphate
-
 ?
2.7.1.221 ATP + N-acetyl-D-muramate
-
 Pseudomonas putida ADP + N-acetyl-alpha-D-muramate 1-phosphate
-
 ?
2.7.1.221 ATP + N-acetyl-D-muramate
-
 Pseudomonas putida DSM 6125 ADP + N-acetyl-alpha-D-muramate 1-phosphate
-
 ?
2.7.1.221 additional information no substrates: N-acetyl-D-mannosamine, N-deacetylated sugars or glucosamine Pseudomonas putida ?
-
 ?
2.7.1.221 additional information no substrates: N-acetyl-D-mannosamine, N-deacetylated sugars or glucosamine Pseudomonas putida DSM 6125 ?
-
 ?
2.7.7.99 additional information no substrates: N-acetyl-D-glucosamine 1-phosphate, N-acetyl-D-galactosamine 1-phosphate Pseudomonas putida ?
-
 ?
2.7.7.99 additional information no substrates: N-acetyl-D-glucosamine 1-phosphate, N-acetyl-D-galactosamine 1-phosphate Pseudomonas putida DSM 6125 ?
-
 ?
2.7.7.99 UTP + N-acetyl-alpha-D-muramate 1-phosphate
-
 Pseudomonas putida UDP-N-acetyl-alpha-D-muramate + diphosphate
-
 ?
2.7.7.99 UTP + N-acetyl-alpha-D-muramate 1-phosphate
-
 Pseudomonas putida DSM 6125 UDP-N-acetyl-alpha-D-muramate + diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.221 amgK
-
 Pseudomonas putida
2.7.1.221 pp_0405
-
 Pseudomonas putida
2.7.7.99 murU
-
 Pseudomonas putida
2.7.7.99 N-acetylmuramate alpha-1-phosphate uridylyltransferase
-
 Pseudomonas putida
2.7.7.99 pp_0406
-
 Pseudomonas putida

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.221 4.38
-
 ATP pH not specified in the publication, temperature not specified in the publication Pseudomonas putida
2.7.1.221 5.15
-
 N-acetyl-D-muramate pH not specified in the publication, temperature not specified in the publication Pseudomonas putida
2.7.1.221 6.64
-
 N-acetyl-D-glucosamine pH not specified in the publication, temperature not specified in the publication Pseudomonas putida

General Information

EC Number General Information Comment Organism
2.7.1.221 physiological function enzyme is involved in a cell wall sugar-recycling route that channels external MurNAc directly to peptidoglycan biosynthesis. A deletion mutant shows increase fosfomycin sensitivity Pseudomonas putida
2.7.7.99 physiological function enzyme is involved in a salvage pathway in Gram-negative bacteria that bypasses de novo biosynthesis of UDP N-acetylmuramic acid. The pathway consisits of anomeric sugar kinase AmgK and the MurNAc alpha-1-phosphate uridylyl transferase MurU. Deletion of the encoding gene increases fosfomycin sensitivity Pseudomonas putida