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Literature summary extracted from

  • Leitsch, D.; Janssen, B.; Kolarich, D.; Johnson, P.; Duchene, M.
    Trichomonas vaginalis flavin reductase 1 and its role in metronidazole resistance (2014), Mol. Microbiol., 91, 198-208 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.1.30 gene TVAG_517010, DNA and amino acid sequence determination and analysis, quantitative RT-PCR expression analysis of FR1 in metronidazole-susceptible isolates C1, G3, JH31A and Tv2 , re-introduction of FR1 into an aerobically-resistant strain, transfection of the highly metronidazole-resistant isolate B7268 with the FR1 gene restores metronidazole susceptibility Trichomonas vaginalis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.30 riboflavin FR1 inhibition by possibly either by the semiquinone or the fully reduced form Trichomonas vaginalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.1.30 FMNH2 + NADP+ Trichomonas vaginalis
-
 FMN + NADPH + H+
-
 r
1.5.1.30 FMNH2 + NADP+ Trichomonas vaginalis G3
-
 FMN + NADPH + H+
-
 r
1.5.1.30 additional information Trichomonas vaginalis FR is a relevant source of hydrogen peroxide but superoxide radical anions are a minor by-product of the reaction ?
-
 ?
1.5.1.30 additional information Trichomonas vaginalis G3 FR is a relevant source of hydrogen peroxide but superoxide radical anions are a minor by-product of the reaction ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.30 Trichomonas vaginalis A2GH85
-
-
1.5.1.30 Trichomonas vaginalis G3 A2GH85
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.1.30 additional information analysis of enzyme FR1 expression in metronidazole-susceptible isolates C1, G3, JH31A and Tv2: the highly metronidazole-resistant cell line of strain C1, C1res, and the clinical isolate B7268 lack detectable FRs. The residual FR activity found in CDC085 and LA1 likely results from alternative FRs that are highly expressed Trichomonas vaginalis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.30 FMNH2 + NADP+
-
 Trichomonas vaginalis FMN + NADPH + H+
-
 r
1.5.1.30 FMNH2 + NADP+
-
 Trichomonas vaginalis G3 FMN + NADPH + H+
-
 r
1.5.1.30 additional information FR is a relevant source of hydrogen peroxide but superoxide radical anions are a minor by-product of the reaction Trichomonas vaginalis ?
-
 ?
1.5.1.30 additional information FR is a relevant source of hydrogen peroxide but superoxide radical anions are a minor by-product of the reaction Trichomonas vaginalis G3 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.5.1.30 flavin reductase 1
-
 Trichomonas vaginalis
1.5.1.30 FR1
-
 Trichomonas vaginalis
1.5.1.30 TVAG_517010
-
 Trichomonas vaginalis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.1.30 37
-
 assay at Trichomonas vaginalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.30 5.5
-
 assay at Trichomonas vaginalis

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.30 additional information FR1 displays only 3% activity when NADH is added instead of NADPH Trichomonas vaginalis
1.5.1.30 NADP+
-
 Trichomonas vaginalis
1.5.1.30 NADPH
-
 Trichomonas vaginalis

General Information

EC Number General Information Comment Organism
1.5.1.30 metabolism Trichomonas vaginalis has two different enzymatic pathways to remove intracellular oxygen, i.e. NADH oxidase, which is inhibited by metronidazole, and flavin reductase Trichomonas vaginalis
1.5.1.30 physiological function regarding all flavin reductase enzymes in Trichomonas vaginalis, FR1 is responsible for the flavin reductase activity. Hydrogen peroxide is the main if not the single product of FR1. FR1 displays a 10 to 20fold higher affinity to FMN than enzymes FR5 and FR6. Also the affinity of FR1 to FAD is at least 10fold higher than observed with FR5 and FR6 but the Vmax is considerably lower (roughly 33% of the Vmax for FMN). In contrast, FR5 and FR6 display higher affinity for riboflavin than FR1, which is inhibited by riboflavin Trichomonas vaginalis