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Literature summary extracted from

  • El-Sayed, A.; Shindia, A.; Zaher, Y.
    Purification and characterization of L-amino acid oxidase from the solid-state grown cultures of Aspergillus oryzae ASH (2013), Microbiology, 82, 762-771 .
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.3.2 EDTA slight inhibition Aspergillus oryzae
1.4.3.2 hydroxylamine
-
 Aspergillus oryzae
1.4.3.2 SDS
-
 Aspergillus oryzae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.2 3.39
-
 L-Lys at pH 7.0 and 30°C Aspergillus oryzae
1.4.3.2 5.38
-
 L-Tyr at pH 7.0 and 30°C Aspergillus oryzae
1.4.3.2 6.63
-
 L-Phe at pH 7.0 and 30°C Aspergillus oryzae
1.4.3.2 15.6
-
 L-Met at pH 7.0 and 30°C Aspergillus oryzae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.3.2 105000 110000 gel filtration Aspergillus oryzae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.3.2 bovine serum albumin + H2O + O2 Aspergillus oryzae 37% activity compared to L-Lys ?
-
 ?
1.4.3.2 casein + H2O + O2 Aspergillus oryzae 59% activity compared to L-Lys ?
-
 ?
1.4.3.2 glutathione + H2O + O2 Aspergillus oryzae 97% activity compared to L-Lys ?
-
 ?
1.4.3.2 Gly + H2O + O2 Aspergillus oryzae 17.8% activity compared to L-Lys glyoxylate + NH3 + H2O2
-
 ?
1.4.3.2 L-Ala + H2O + O2 Aspergillus oryzae 21.2% activity compared to L-Lys 2-oxopropanoic acid + NH3 + H2O2
-
 ?
1.4.3.2 L-Ala + H2O + O2 Aspergillus oryzae ASH 21.2% activity compared to L-Lys 2-oxopropanoic acid + NH3 + H2O2
-
 ?
1.4.3.2 L-Arg + H2O + O2 Aspergillus oryzae 27.6% activity compared to L-Lys 2-oxo-5-guanidinovaleric acid + NH3 + H2O2
-
 ?
1.4.3.2 L-Arg + H2O + O2 Aspergillus oryzae ASH 27.6% activity compared to L-Lys 2-oxo-5-guanidinovaleric acid + NH3 + H2O2
-
 ?
1.4.3.2 L-Asp + H2O + O2 Aspergillus oryzae 19.8% activity compared to L-Lys oxaloacetate + NH3 + H2O2
-
 ?
1.4.3.2 L-Cys + H2O + O2 Aspergillus oryzae 29.5% activity compared to L-Lys 2-oxo-3-mercaptopropanoic acid + NH3 + H2O2
-
 ?
1.4.3.2 L-Glu + H2O + O2 Aspergillus oryzae 15.5% activity compared to L-Lys 2-oxoglutarate + NH3 + H2O2
-
 ?
1.4.3.2 L-Glu + H2O + O2 Aspergillus oryzae ASH 15.5% activity compared to L-Lys 2-oxoglutarate + NH3 + H2O2
-
 ?
1.4.3.2 L-Lys + H2O + O2 Aspergillus oryzae 100% activity 6-amino-2-oxo-hexanoic acid + NH3 + H2O2
-
 ?
1.4.3.2 L-Met + H2O + O2 Aspergillus oryzae 101% activity compared to L-Lys 4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
-
 ?
1.4.3.2 L-Met + H2O + O2 Aspergillus oryzae ASH 101% activity compared to L-Lys 4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
-
 ?
1.4.3.2 L-Phe + H2O + O2 Aspergillus oryzae 81.3% activity compared to L-Lys phenylpyruvate + NH3 + H2O2
-
 ?
1.4.3.2 L-Phe + H2O + O2 Aspergillus oryzae ASH 81.3% activity compared to L-Lys phenylpyruvate + NH3 + H2O2
-
 ?
1.4.3.2 L-Tyr + H2O + O2 Aspergillus oryzae 104% activity compared to L-Lys 4-hydroxyphenylpyruvate + NH3 + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.2 Aspergillus oryzae
-
-
-
1.4.3.2 Aspergillus oryzae ASH
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.2 ammonium sulfate precipitation, DEAE-cellulose column chromatography, and Sephadex G200 gel filtration Aspergillus oryzae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.2 bovine serum albumin + H2O + O2 37% activity compared to L-Lys Aspergillus oryzae ?
-
 ?
1.4.3.2 casein + H2O + O2 59% activity compared to L-Lys Aspergillus oryzae ?
-
 ?
1.4.3.2 glutathione + H2O + O2 97% activity compared to L-Lys Aspergillus oryzae ?
-
 ?
1.4.3.2 Gly + H2O + O2 17.8% activity compared to L-Lys Aspergillus oryzae glyoxylate + NH3 + H2O2
-
 ?
1.4.3.2 L-Ala + H2O + O2 21.2% activity compared to L-Lys Aspergillus oryzae 2-oxopropanoic acid + NH3 + H2O2
-
 ?
1.4.3.2 L-Ala + H2O + O2 21.2% activity compared to L-Lys Aspergillus oryzae ASH 2-oxopropanoic acid + NH3 + H2O2
-
 ?
1.4.3.2 L-Arg + H2O + O2 27.6% activity compared to L-Lys Aspergillus oryzae 2-oxo-5-guanidinovaleric acid + NH3 + H2O2
-
 ?
1.4.3.2 L-Arg + H2O + O2 27.6% activity compared to L-Lys Aspergillus oryzae ASH 2-oxo-5-guanidinovaleric acid + NH3 + H2O2
-
 ?
1.4.3.2 L-Asp + H2O + O2 19.8% activity compared to L-Lys Aspergillus oryzae oxaloacetate + NH3 + H2O2
-
 ?
1.4.3.2 L-Cys + H2O + O2 29.5% activity compared to L-Lys Aspergillus oryzae 2-oxo-3-mercaptopropanoic acid + NH3 + H2O2
-
 ?
1.4.3.2 L-Glu + H2O + O2 15.5% activity compared to L-Lys Aspergillus oryzae 2-oxoglutarate + NH3 + H2O2
-
 ?
1.4.3.2 L-Glu + H2O + O2 15.5% activity compared to L-Lys Aspergillus oryzae ASH 2-oxoglutarate + NH3 + H2O2
-
 ?
1.4.3.2 L-Lys + H2O + O2 100% activity Aspergillus oryzae 6-amino-2-oxo-hexanoic acid + NH3 + H2O2
-
 ?
1.4.3.2 L-Met + H2O + O2 101% activity compared to L-Lys Aspergillus oryzae 4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
-
 ?
1.4.3.2 L-Met + H2O + O2 101% activity compared to L-Lys Aspergillus oryzae ASH 4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
-
 ?
1.4.3.2 L-Phe + H2O + O2 81.3% activity compared to L-Lys Aspergillus oryzae phenylpyruvate + NH3 + H2O2
-
 ?
1.4.3.2 L-Phe + H2O + O2 81.3% activity compared to L-Lys Aspergillus oryzae ASH phenylpyruvate + NH3 + H2O2
-
 ?
1.4.3.2 L-Tyr + H2O + O2 104% activity compared to L-Lys Aspergillus oryzae 4-hydroxyphenylpyruvate + NH3 + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.3.2 homodimer 2 * 55000, SDS-PAGE Aspergillus oryzae

Synonyms

EC Number Synonyms Comment Organism
1.4.3.2 L-AAO
-
 Aspergillus oryzae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.3.2 55
-
-
 Aspergillus oryzae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.3.2 20 60 the enzyme has half-life values of 21.2, 8.3, 3.6, 3.1, 2.6 h at 30, 35, 40, 50, 60°C, respectively, and melting temperature of 61.3°C Aspergillus oryzae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4.3.2 0.036
-
 L-Tyr at pH 7.0 and 30°C Aspergillus oryzae
1.4.3.2 0.039
-
 L-Phe at pH 7.0 and 30°C Aspergillus oryzae
1.4.3.2 0.039
-
 L-Met at pH 7.0 and 30°C Aspergillus oryzae
1.4.3.2 0.04
-
 L-Lys at pH 7.0 and 30°C Aspergillus oryzae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.3.2 7.8 8
-
 Aspergillus oryzae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.4.3.2 5 10.4 the enzyme shows a slight decrease in its activity at pH 5.0 and 9.6. Actually, the enzyme activity decreases by about 50% of its initial activity at the optimum at acidic pH (3.0) and by less than 3% at alkaline one (pH 10.4) Aspergillus oryzae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.4.3.2 6 9.5 the enzyme exhibits a broad pH stability (6.0-9.5), with the highest stability from pH 7.2-7.6 in potassium phosphate buffer Aspergillus oryzae

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.2 FAD
-
 Aspergillus oryzae

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.4.3.2 Aspergillus oryzae isoelectric focusing
-
 4.15