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Literature summary extracted from

  • Bhandiwad, A.; Shaw, A.; Guss, A.; Guseva, A.; Bahl, H.; Lynd, L.
    Metabolic engineering of Thermoanaerobacterium saccharolyticum for n-butanol production (2014), Metab. Eng., 21, 17-25 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.57 gene bcd, the genes (crt, bcd, etfB, etfA, hbd, and thl) responsible for butyryl-CoA formation occur as a multi-gene operon, plasmid construction map for expressing individual genes of the n-butanol pathway, plasmids pHBD,pCRT,pBCD-etfAB, and pADHE, overview Thermoanaerobacterium saccharolyticum

Protein Variants

EC Number Protein Variants Comment Organism
1.2.1.57 additional information recombinant expression of the enzyme from Clostridium acetobutylicum strain ATCC 824 in Thermoanaerobacterium saccharolyticum strain DSM 8691 Thermoanaerobacterium saccharolyticum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.57 butanoyl-CoA + NADH + H+ Thermoanaerobacterium saccharolyticum
-
 butanal + CoA + NAD+
-
 ?
1.2.1.57 butanoyl-CoA + NADH + H+ Thermoanaerobacterium saccharolyticum DSM 571
-
 butanal + CoA + NAD+
-
 ?
1.2.1.57 butanoyl-CoA + NADPH + H+ Thermoanaerobacterium saccharolyticum
-
 butanal + CoA + NADP+
-
 ?
1.2.1.57 butanoyl-CoA + NADPH + H+ Thermoanaerobacterium saccharolyticum DSM 571
-
 butanal + CoA + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.57 Thermoanaerobacterium saccharolyticum
-
 formerly Clostridium thermosaccharolyticum
-
1.2.1.57 Thermoanaerobacterium saccharolyticum DSM 571
-
 formerly Clostridium thermosaccharolyticum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.57 0.67
-
 pH 7.0, 55°C, substrate butanoyl-CoA Thermoanaerobacterium saccharolyticum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.57 butanoyl-CoA + FADH2
-
 Thermoanaerobacterium saccharolyticum butanal + CoA + FAD
-
 ?
1.2.1.57 butanoyl-CoA + FADH2
-
 Thermoanaerobacterium saccharolyticum DSM 571 butanal + CoA + FAD
-
 ?
1.2.1.57 butanoyl-CoA + NADH + H+
-
 Thermoanaerobacterium saccharolyticum butanal + CoA + NAD+
-
 ?
1.2.1.57 butanoyl-CoA + NADH + H+
-
 Thermoanaerobacterium saccharolyticum DSM 571 butanal + CoA + NAD+
-
 ?
1.2.1.57 butanoyl-CoA + NADPH + H+
-
 Thermoanaerobacterium saccharolyticum butanal + CoA + NADP+
-
 ?
1.2.1.57 butanoyl-CoA + NADPH + H+
-
 Thermoanaerobacterium saccharolyticum DSM 571 butanal + CoA + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.2.1.57 BCD
-
 Thermoanaerobacterium saccharolyticum
1.2.1.57 butyryl CoA dehydrogenase
-
 Thermoanaerobacterium saccharolyticum
1.2.1.57 Tthe_1660
-
 Thermoanaerobacterium saccharolyticum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.1.57 55
-
 assay at Thermoanaerobacterium saccharolyticum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.57 7
-
 assay at Thermoanaerobacterium saccharolyticum

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.57 NADH
-
 Thermoanaerobacterium saccharolyticum
1.2.1.57 NADPH
-
 Thermoanaerobacterium saccharolyticum