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Literature summary extracted from
- A ferredoxin- and F420H2-dependent, electron-bifurcating, heterodisulfide reductase with homologs in the domains Bacteria and Archaea (2017), mBio, 8, e02285-16 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.8.7.3 | expressed in Escherichia coli BL21(DE3) cells | Methanosarcina acetivorans |
| 1.8.98.1 | expressed in Escherichia coli BL21(DE3) cells | Methanosarcina acetivorans |
| 1.8.98.4 | expressed in Escherichia coli BL21(DE3) cells | Methanosarcina acetivorans |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.5.7.2 | 0.0064 | - |
oxidized coenzyme F420 | 21°C, pH not specified in the publication | Methanosarcina acetivorans |  |
| 1.8.7.3 | 0.05 | - |
CoM-S-S-CoB | subunit HdrB2C2, at 21°C, pH not specified in the publication | Methanosarcina acetivorans | |
| 1.8.7.3 | 1.1 | - |
CoB | subunit HdrB2, at 21°C, pH not specified in the publication | Methanosarcina acetivorans | |
| 1.8.7.3 | 1.2 | - |
CoM | subunit HdrB2, at 21°C, pH not specified in the publication | Methanosarcina acetivorans | |
| 1.8.7.3 | 1.2 | - |
CoB | subunit HdrB2C2, at 21°C, pH not specified in the publication | Methanosarcina acetivorans | |
| 1.8.7.3 | 1.4 | - |
CoM | subunit HdrB2C2, at 21°C, pH not specified in the publication | Methanosarcina acetivorans | |
| 1.8.98.1 | 1.1 | - |
coenzyme B | subunit HdrB2, at 21°C, pH not specified in the publication | Methanosarcina acetivorans | |
| 1.8.98.1 | 1.2 | - |
coenzyme M | subunit HdrB2, at 21°C, pH not specified in the publication | Methanosarcina acetivorans | |
| 1.8.98.1 | 1.2 | - |
coenzyme B | subunit HdrB2C2, at 21°C, pH not specified in the publication | Methanosarcina acetivorans | |
| 1.8.98.1 | 1.4 | - |
coenzyme M | subunit HdrB2C2, at 21°C, pH not specified in the publication | Methanosarcina acetivorans | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.7.3 | Zn2+ | required | Methanosarcina acetivorans | |
| 1.8.98.1 | Zn2+ | subunit HdrB2 contains zinc | Methanosarcina acetivorans | |
| 1.8.98.4 | Zn2+ | required | Methanosarcina acetivorans | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.7.3 | oxidized ferredoxin [iron-sulfur] cluster + CoB + CoM | Methanosarcina acetivorans | - |
reduced ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB + H+ | - |
r | |
| 1.8.7.3 | reduced ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB + H+ | Methanosarcina acetivorans | - |
oxidized ferredoxin [iron-sulfur] cluster + CoB + CoM | - |
r | |
| 1.8.98.4 | oxidized coenzyme F420 + reduced ferredoxin [iron-sulfur] cluster + CoB + CoM + H+ | Methanosarcina acetivorans | - |
reduced coenzyme F420 + oxidized ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.5.7.2 | Methanosarcina acetivorans | Q8TM02 | cf. EC 1.8.7.3 | - |
| 1.5.7.2 | Methanosarcina acetivorans ATCC 35395 | Q8TM02 | cf. EC 1.8.7.3 | - |
| 1.8.7.3 | Methanosarcina acetivorans | - |
- |
- |
| 1.8.98.1 | Methanosarcina acetivorans | - |
- |
- |
| 1.8.98.4 | Methanosarcina acetivorans | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.8.7.3 | - |
Methanosarcina acetivorans |
| 1.8.98.4 | - |
Methanosarcina acetivorans |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.5.7.2 | oxidized coenzyme F420 + 2 reduced ferredoxin + 2 H+ | - |
Methanosarcina acetivorans | reduced coenzyme F420 + 2 oxidized ferredoxin | - |
? | |
| 1.5.7.2 | oxidized coenzyme F420 + 2 reduced ferredoxin + 2 H+ | - |
Methanosarcina acetivorans ATCC 35395 | reduced coenzyme F420 + 2 oxidized ferredoxin | - |
? | |
| 1.8.7.3 | oxidized ferredoxin [iron-sulfur] cluster + CoB + CoM | - |
Methanosarcina acetivorans | reduced ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB + H+ | - |
r | |
| 1.8.7.3 | reduced ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB + H+ | - |
Methanosarcina acetivorans | oxidized ferredoxin [iron-sulfur] cluster + CoB + CoM | - |
r | |
| 1.8.98.1 | coenzyme B + coenzyme M + reduced ferredoxin + H+ + coenzyme F420 | - |
Methanosarcina acetivorans | coenzyme B-coenzyme M disulfide + ferredoxin + H2 + reduced coenzyme F420 | - |
? | |
| 1.8.98.4 | oxidized coenzyme F420 + reduced ferredoxin [iron-sulfur] cluster + CoB + CoM + H+ | - |
Methanosarcina acetivorans | reduced coenzyme F420 + oxidized ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.8.7.3 | ? | x * 18000, subunit HdrC2, SDS-PAGE | Methanosarcina acetivorans |
| 1.8.7.3 | ? | x * 33000, subunit HdrB2, SDS-PAGE | Methanosarcina acetivorans |
| 1.8.98.1 | ? | x * 18000, subunit HdrC2, SDS-PAGE | Methanosarcina acetivorans |
| 1.8.98.1 | ? | x * 33000, subunit HdrB2, SDS-PAGE | Methanosarcina acetivorans |
| 1.8.98.1 | ? | x * 87000, subunit HdrA2, SDS-PAGE | Methanosarcina acetivorans |
| 1.8.98.4 | ? | x * 87000, subunit HdrA2, SDS-PAGE | Methanosarcina acetivorans |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.5.7.2 | ferredoxin/F(420)H(2)-dependent CoB-CoM heterodisulfide reductase subunit A | - |
Methanosarcina acetivorans |
| 1.5.7.2 | HdrA2 | - |
Methanosarcina acetivorans |
| 1.8.7.3 | HdrB2 | subunit | Methanosarcina acetivorans |
| 1.8.7.3 | HdrB2C2 | subunit | Methanosarcina acetivorans |
| 1.8.98.1 | Fdx:CoMS-SCoB oxidoreductase | - |
Methanosarcina acetivorans |
| 1.8.98.1 | ferredoxin- and F420H2-dependent, electron-bifurcating, heterodisulfide reductase | - |
Methanosarcina acetivorans |
| 1.8.98.1 | HDR | - |
Methanosarcina acetivorans |
| 1.8.98.1 | hdrA2B2C2 | - |
Methanosarcina acetivorans |
| 1.8.98.1 | heterodisulfide reductase | - |
Methanosarcina acetivorans |
| 1.8.98.4 | HdrA2 | subunit | Methanosarcina acetivorans |
| 1.8.98.4 | hdrA2B2C2 | - |
Methanosarcina acetivorans |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.7.3 | [4Fe-4S]-center | - |
Methanosarcina acetivorans | |
| 1.8.98.1 | coenzyme F420 | - |
Methanosarcina acetivorans | |
| 1.8.98.1 | FAD | - |
Methanosarcina acetivorans | |
| 1.8.98.1 | [2Fe-2S]-center | - |
Methanosarcina acetivorans | |
| 1.8.98.1 | [4Fe-4S]-center | - |
Methanosarcina acetivorans | |
| 1.8.98.4 | [4Fe-4S]-center | - |
Methanosarcina acetivorans | |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.5.7.2 | physiological function | presence of an intersubunit electron transport pathway in heterodisulfide reductase HdrA2B2C2 dependent on ferredoxin or coenzyme F420 (F420H2) as an electron donor. HdrA2B2C2 catalyzes a thermodynamically favorable, flavin-based, coupling of the endergonic one-electron reduction of ferredoxin (E0 of ca. -520 mV) with F420H2 (E0 of -380 mV) to the exergonic reduction of CoMS-SCoB (E0 of -140 mV) | Methanosarcina acetivorans |
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