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Literature summary extracted from

  • Hassan, H.; Zein Eldein, A.; Rizk, N.
    Cloning and kinetic properties of Catechol 2,3-dioxygenase from novel alkaliphilic BTEX-degrading Pseudomonas sp. HB01 (2014), Life Sci. J., 11, 376-384 .
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.2 expressed in Escherichia coli DH5alpha cells Pseudomonas sp. HB01

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.2 3-chlorocatechol
-
 Pseudomonas sp. HB01

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.2 0.0003
-
 2,2',3-trihydroxybiphenylether pH and temperature not specified in the publication Pseudomonas sp. HB01
1.13.11.2 0.0012
-
 2,3-Dihydroxybiphenyl pH and temperature not specified in the publication Pseudomonas sp. HB01
1.13.11.2 0.03
-
 3-methylcatechol pH and temperature not specified in the publication Pseudomonas sp. HB01
1.13.11.2 0.3
-
 catechol pH and temperature not specified in the publication Pseudomonas sp. HB01

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.2 catechol + O2 Pseudomonas sp. HB01
-
 2-hydroxymuconate-6-semialdehyde
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.2 Pseudomonas sp. HB01 T1T1S4
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.2 2,2',3-trihydroxybiphenylether + O2
-
 Pseudomonas sp. HB01 ?
-
 ?
1.13.11.2 2,3-dihydroxybiphenyl + O2
-
 Pseudomonas sp. HB01 ?
-
 ?
1.13.11.2 3-methylcatechol + O2
-
 Pseudomonas sp. HB01 2-hydroxy-6-oxohepta-2,4-dienoate
-
 ?
1.13.11.2 4-chlorocatechol + O2
-
 Pseudomonas sp. HB01 5-chloro-2-hydroxymuconic semialdehyde
-
 ?
1.13.11.2 catechol + O2
-
 Pseudomonas sp. HB01 2-hydroxymuconate-6-semialdehyde
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.2 C2,3O
-
 Pseudomonas sp. HB01
1.13.11.2 catechol 2,3 dioxygenase
-
 Pseudomonas sp. HB01

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.2 60
-
 2,2',3-trihydroxybiphenylether pH and temperature not specified in the publication Pseudomonas sp. HB01
1.13.11.2 150
-
 3-methylcatechol pH and temperature not specified in the publication Pseudomonas sp. HB01
1.13.11.2 200
-
 2,3-Dihydroxybiphenyl pH and temperature not specified in the publication Pseudomonas sp. HB01
1.13.11.2 360
-
 catechol pH and temperature not specified in the publication Pseudomonas sp. HB01