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Literature summary extracted from

  • Equar, M.Y.; Tani, Y.; Mihara, H.
    Purification and properties of glycine oxidase from Pseudomonas putida KT2440 (2015), J. Nutr. Sci. Vitaminol., 61, 506-510 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.3.19 expressed in Escherichia coli BL21(DE3) cells Pseudomonas putida

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.3.19 additional information not inhibited by glycine, sarcosine, and glycine ethyl ester Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.19 2.43
-
 glycine at pH 8.5 and 40°C Pseudomonas putida
1.4.3.19 4.86
-
 sarcosine at pH 8.5 and 40°C Pseudomonas putida
1.4.3.19 7.68
-
 N-ethylglycine at pH 8.5 and 40°C Pseudomonas putida
1.4.3.19 31.7
-
 D-proline at pH 8.5 and 40°C Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.3.19 glycine + H2O + O2 Pseudomonas putida
-
 glyoxylate + NH3 + H2O2
-
 ?
1.4.3.19 glycine + H2O + O2 Pseudomonas putida KT 2240
-
 glyoxylate + NH3 + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.19 Pseudomonas putida Q88Q83
-
-
1.4.3.19 Pseudomonas putida KT 2240 Q88Q83
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.19 D-alanine + H2O + O2 10% activity compared to glycine Pseudomonas putida ?
-
 ?
1.4.3.19 D-alanine + H2O + O2 10% activity compared to glycine Pseudomonas putida KT 2240 ?
-
 ?
1.4.3.19 D-proline + H2O + O2 25% activity compared to glycine Pseudomonas putida ?
-
 ?
1.4.3.19 D-proline + H2O + O2 25% activity compared to glycine Pseudomonas putida KT 2240 ?
-
 ?
1.4.3.19 glycine + H2O + O2
-
 Pseudomonas putida glyoxylate + NH3 + H2O2
-
 ?
1.4.3.19 glycine + H2O + O2
-
 Pseudomonas putida KT 2240 glyoxylate + NH3 + H2O2
-
 ?
1.4.3.19 glycine ethyl ester + H2O + O2 5% activity compared to glycine Pseudomonas putida ?
-
 ?
1.4.3.19 glycine ethyl ester + H2O + O2 5% activity compared to glycine Pseudomonas putida KT 2240 ?
-
 ?
1.4.3.19 glycylglycine + H2O + O2 3% activity compared to glycine Pseudomonas putida ?
-
 ?
1.4.3.19 N-ethylglycine + H2O + O2 40% activity compared to glycine Pseudomonas putida ?
-
 ?
1.4.3.19 sarcosine + H2O + O2
-
 Pseudomonas putida ?
-
 ?
1.4.3.19 sarcosine + H2O + O2
-
 Pseudomonas putida KT 2240 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.3.19 monomer
-
 Pseudomonas putida

Synonyms

EC Number Synonyms Comment Organism
1.4.3.19 ThiO
-
 Pseudomonas putida

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.3.19 40
-
-
 Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.3.19 8.5
-
-
 Pseudomonas putida

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.4.3.19 8 10 the enzyme is most stable in the pH range from 8.0 to 10.0 upon incubation at 25°C for 1 h Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.19 FAD
-
 Pseudomonas putida