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Literature summary extracted from

  • Qu, Y.; Kong, C.; Zhou, H.; Shen, E.; Wang, J.; Shen, W.; Zhang, X.; Zhang, Z.; Ma, Q.; Zhou, J.
    Catalytic properties of 2,3-dihydroxybiphenyl 1,2-dioxygenase from Dyella ginsengisoli LA-4 immobilized on mesoporous silica SBA-15 (2014), J. Mol. Catal. B, 99, 136-142 .
No PubMed abstract available

Protein Variants

EC Number Protein Variants Comment Organism
1.13.11.39 additional information immobilization of the enzyme BphC on mesoporous silica SBA-15 in order to improve its stability with relatively high retaining activities. By Fourier transformed infrared spectroscopy (FTIR) and N2 adsorption/desorption isotherms, BphC is confirmed to be successfully adsorbed and captured on SBA-15. Under the experimental conditions, the maximum loading amount reaches 124.6 mg protein/g support at pH 6.0. The immobilized BphC is more stable at 4°C than the free enzyme and the thermostability of BphC is significantly improved by immobilization compared to the wild-type enzyme, while the catalytic efficiency with biphenyl-2,3-diol is reduced. The activity and stability alteration occur due to the changes of secondary structure of BphC. Mechanism of immobilization Dyella ginsengisoli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.39 additional information
-
 additional information Michaelis-Menten kinetics Dyella ginsengisoli
1.13.11.39 0.00364
-
 biphenyl-2,3-diol pH 8.0, 30°C, free enzyme Dyella ginsengisoli
1.13.11.39 0.01022
-
 biphenyl-2,3-diol pH 8.0, 30°C, immobilized enzyme Dyella ginsengisoli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.39 Fe2+ required Dyella ginsengisoli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.39 biphenyl-2,3-diol + O2 Dyella ginsengisoli
-
 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate
-
 ?
1.13.11.39 biphenyl-2,3-diol + O2 Dyella ginsengisoli LA-4
-
 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.39 Dyella ginsengisoli
-
-
-
1.13.11.39 Dyella ginsengisoli LA-4
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.39 10.74
-
 pH 8.0, 30°C, immobilized enzyme, substrate biphenyl-2,3-diol Dyella ginsengisoli

Storage Stability

EC Number Storage Stability Organism
1.13.11.39 4°C, purified immobilized enzyme BphC,16% of its initial activity after 5 days, the purified free enzyme loses its activity rapidly within 12 h Dyella ginsengisoli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.39 3-methylcatechol + O2
-
 Dyella ginsengisoli 2-hydroxy-6-oxohepta-2,4-dienoate
-
 ?
1.13.11.39 3-methylcatechol + O2
-
 Dyella ginsengisoli LA-4 2-hydroxy-6-oxohepta-2,4-dienoate
-
 ?
1.13.11.39 biphenyl-2,3-diol + O2
-
 Dyella ginsengisoli 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate
-
 ?
1.13.11.39 biphenyl-2,3-diol + O2
-
 Dyella ginsengisoli LA-4 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate
-
 ?
1.13.11.39 catechol + O2
-
 Dyella ginsengisoli 2-hydroxymuconate semialdehyde
-
 ?
1.13.11.39 catechol + O2
-
 Dyella ginsengisoli LA-4 2-hydroxymuconate semialdehyde
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.39 2,3-dihydroxybiphenyl 1,2-dioxygenase
-
 Dyella ginsengisoli
1.13.11.39 BphC
-
 Dyella ginsengisoli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.39 40
-
 free enzyme Dyella ginsengisoli
1.13.11.39 45
-
 immobilized enzyme Dyella ginsengisoli

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.13.11.39 15 55 activity range of immobilized enzyme Dyella ginsengisoli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.13.11.39 40
-
 purified immobilized BphC possess 90% of its initial activity after incubating for 4 h Dyella ginsengisoli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.39 20.63
-
 biphenyl-2,3-diol pH 8.0, 30°C, immobilized enzyme Dyella ginsengisoli
1.13.11.39 79.65
-
 biphenyl-2,3-diol pH 8.0, 30°C, free enzyme Dyella ginsengisoli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.39 8
-
 immobilized enzyme Dyella ginsengisoli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.13.11.39 6 10 activity range of immobilized enzyme Dyella ginsengisoli

General Information

EC Number General Information Comment Organism
1.13.11.39 additional information enzyme structure molecular simulation, overview Dyella ginsengisoli