EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.10.3.2 | Ba2+ | 1 mM, 11% loss of activity | Bacillus sp. (in: Bacteria) | |
1.10.3.2 | EDTA | 1 mM, 15.6% loss of activity | Bacillus sp. (in: Bacteria) | |
1.10.3.2 | L-cysteine | 1 mM, reduced 87% of its original activity | Bacillus sp. (in: Bacteria) | |
1.10.3.2 | additional information | the enzyme can tolerate up to 600-800 mM NaCl. NaN3 (1 mM) has almost no effect on the laccase activity | Bacillus sp. (in: Bacteria) | |
1.10.3.2 | SDS | 1 mM, 38.6% loss of activity | Bacillus sp. (in: Bacteria) |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.10.3.2 | 0.0037 | - |
syringaldazine | pH 8.0, 22°C | Bacillus sp. (in: Bacteria) | |
1.10.3.2 | 0.1327 | - |
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | pH 5.0, 22°C | Bacillus sp. (in: Bacteria) |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.10.3.2 | extracellular | - |
Bacillus sp. (in: Bacteria) | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.10.3.2 | Na+ | up to 400 mM NaCl stimulates laccase activity. At 600 to 800 mM NaCl, the laccase retains all of its original activity and at higher concentrations, the activity decreases | Bacillus sp. (in: Bacteria) | |
1.10.3.2 | NaCl | halotolerant with maximum activity at 100 mM NaCl | Bacillus sp. (in: Bacteria) | |
1.10.3.2 | Ni2+ | 1 mM, 12% increase in activity | Bacillus sp. (in: Bacteria) |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.10.3.2 | 180000 | - |
SDS-PAGE | Bacillus sp. (in: Bacteria) |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.10.3.2 | Bacillus sp. (in: Bacteria) | - |
- |
- |
1.10.3.2 | Bacillus sp. (in: Bacteria) WT | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.10.3.2 | - |
Bacillus sp. (in: Bacteria) |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.10.3.2 | 1 | - |
substrate: syringaldazine, pH 8.0, 22°C | Bacillus sp. (in: Bacteria) |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.10.3.2 | 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2 | - |
Bacillus sp. (in: Bacteria) | ? | - |
? | |
1.10.3.2 | 2,6-dimethoxyphenol + O2 | 75.8% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Bacillus sp. (in: Bacteria) | ? | - |
? | |
1.10.3.2 | alpha-naphthol + O2 | 22.0% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Bacillus sp. (in: Bacteria) | ? | - |
? | |
1.10.3.2 | catechol + O2 | 27.5% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Bacillus sp. (in: Bacteria) | ? | - |
? | |
1.10.3.2 | catechol + O2 | 27.5% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Bacillus sp. (in: Bacteria) WT | ? | - |
? | |
1.10.3.2 | guaiacol + O2 | 90.6% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Bacillus sp. (in: Bacteria) | ? | - |
? | |
1.10.3.2 | L-DOPA + O2 | 13.7% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Bacillus sp. (in: Bacteria) | ? | - |
? | |
1.10.3.2 | additional information | decolorization of azo dyes: the enzyme decolorizes sulphonyl green BLE up to 97% at pH 7.0 after 2 h incubation at 35°C, without any addition of mediators. No activity with resorcinol and tyrosine | Bacillus sp. (in: Bacteria) | ? | - |
? | |
1.10.3.2 | additional information | decolorization of azo dyes: the enzyme decolorizes sulphonyl green BLE up to 97% at pH 7.0 after 2 h incubation at 35°C, without any addition of mediators. No activity with resorcinol and tyrosine | Bacillus sp. (in: Bacteria) WT | ? | - |
? | |
1.10.3.2 | pyrogallol + O2 | 95.2% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Bacillus sp. (in: Bacteria) | ? | - |
? | |
1.10.3.2 | pyrogallol + O2 | 95.2% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Bacillus sp. (in: Bacteria) WT | ? | - |
? | |
1.10.3.2 | syringaldazine + O2 | - |
Bacillus sp. (in: Bacteria) | ? | - |
? | |
1.10.3.2 | syringaldazine + O2 | - |
Bacillus sp. (in: Bacteria) WT | ? | - |
? | |
1.10.3.2 | tannic acid + O2 | 17.9% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Bacillus sp. (in: Bacteria) | ? | - |
? | |
1.10.3.2 | tannic acid + O2 | 17.9% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Bacillus sp. (in: Bacteria) WT | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.10.3.2 | ? | x * 180000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.10.3.2 | 55 | - |
substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) or syringaldazine | Bacillus sp. (in: Bacteria) |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.10.3.2 | 50 | 80 | 50°C: about 20% of maximal activity, 90°C: about 40% of maximal activity, substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Bacillus sp. (in: Bacteria) |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.10.3.2 | 50 | - |
a significant increase in activity is observed until 60 min, in which the relative activity reaches to 150% | Bacillus sp. (in: Bacteria) |
1.10.3.2 | 60 | - |
after 15 min incubation relative activity reaches to 140% | Bacillus sp. (in: Bacteria) |
1.10.3.2 | 70 | - |
after 15 min incubation relative activity reaches to 148% | Bacillus sp. (in: Bacteria) |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.10.3.2 | 51 | - |
syringaldazine | pH 5.0, 22°C | Bacillus sp. (in: Bacteria) | |
1.10.3.2 | 309 | - |
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | pH 5.0, 22°C | Bacillus sp. (in: Bacteria) |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.10.3.2 | 5 | - |
substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Bacillus sp. (in: Bacteria) |
1.10.3.2 | 8 | - |
substrate: syringaldazine | Bacillus sp. (in: Bacteria) |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.10.3.2 | 4 | 6 | pH 4.0: about 70% of maximal activity, pH 6.0: about 85% of maximal activity, substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Bacillus sp. (in: Bacteria) |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.10.3.2 | 2328 | - |
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | pH 5.0, 22°C | Bacillus sp. (in: Bacteria) | |
1.10.3.2 | 13783 | - |
syringaldazine | pH 8.0, 22°C | Bacillus sp. (in: Bacteria) |