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Literature summary extracted from

  • Karich, A.; Scheibner, K.; Ullrich, R.; Hofrichter, M.
    Exploring the catalase activity of unspecific peroxygenases and the mechanism of peroxide-dependent heme destruction (2016), J. Mol. Catal. B, 134, 238-246.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.11.2.1 H2O2 at pH 5.0, the enzyme shows 50% residual activity at 5 mM H2O2, and at pH 8.0, the enzyme shows 40% residual activity at 5 mM H2O2 Coprinopsis cinerea
1.11.2.1 H2O2 after exposition to 2 mM H2O2, the enzyme does not exceed 60% residual activity Cyclocybe aegerita
1.11.2.1 H2O2 after exposition to 2 mM H2O2, the enzyme does not exceed 60% residual activity Marasmius rotula

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.2.1 0.79
-
H2O2 at pH 7.0, temperature not specified in the publication Cyclocybe aegerita
1.11.2.1 3.1
-
veratryl alcohol at pH 7.0, temperature not specified in the publication Cyclocybe aegerita
1.11.2.1 11.4
-
H2O2 at pH 7.0, temperature not specified in the publication Coprinopsis cinerea
1.11.2.1 28.94
-
H2O2 at pH 7.0, temperature not specified in the publication Marasmius rotula

Organism

EC Number Organism UniProt Comment Textmining
1.11.2.1 Coprinopsis cinerea
-
-
-
1.11.2.1 Cyclocybe aegerita B9W4V6
-
-
1.11.2.1 Marasmius rotula
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.2.1 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2
-
Marasmius rotula oxidized 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O
-
?
1.11.2.1 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2
-
Cyclocybe aegerita oxidized 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O
-
?
1.11.2.1 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2
-
Coprinopsis cinerea oxidized 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O
-
?
1.11.2.1 methyl benzoate + H2O2
-
Marasmius rotula ?
-
?
1.11.2.1 methyl benzoate + H2O2
-
Cyclocybe aegerita ?
-
?
1.11.2.1 methyl benzoate + H2O2
-
Coprinopsis cinerea ?
-
?
1.11.2.1 veratryl alcohol + H2O2
-
Marasmius rotula veratraldehyde + H2O
-
?
1.11.2.1 veratryl alcohol + H2O2
-
Cyclocybe aegerita veratraldehyde + H2O
-
?
1.11.2.1 veratryl alcohol + H2O2
-
Coprinopsis cinerea veratraldehyde + H2O
-
?

Synonyms

EC Number Synonyms Comment Organism
1.11.2.1 UPO
-
Marasmius rotula
1.11.2.1 UPO
-
Cyclocybe aegerita
1.11.2.1 UPO
-
Coprinopsis cinerea

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.11.2.1 3 6 veratryl alcohol at pH 7.0, temperature not specified in the publication Cyclocybe aegerita
1.11.2.1 127
-
H2O2 at pH 7.0, temperature not specified in the publication Cyclocybe aegerita
1.11.2.1 1665
-
H2O2 at pH 7.0, temperature not specified in the publication Marasmius rotula
1.11.2.1 1910
-
H2O2 at pH 7.0, temperature not specified in the publication Coprinopsis cinerea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.2.1 5.5
-
-
Marasmius rotula
1.11.2.1 7
-
-
Cyclocybe aegerita
1.11.2.1 7
-
-
Coprinopsis cinerea

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.11.2.1 4 8 more than 50% activity between pH 4.0 and 8.0 Marasmius rotula
1.11.2.1 4 7.5 more than 50% activity between pH 4.0 and 7.5 Coprinopsis cinerea
1.11.2.1 5.5 7.5 more than 50% activity between pH 5.5 and 7.5 Cyclocybe aegerita

Cofactor

EC Number Cofactor Comment Organism Structure
1.11.2.1 heme
-
Marasmius rotula
1.11.2.1 heme
-
Cyclocybe aegerita
1.11.2.1 heme
-
Coprinopsis cinerea

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.11.2.1 11.6
-
veratryl alcohol at pH 7.0, temperature not specified in the publication Cyclocybe aegerita
1.11.2.1 57.5
-
H2O2 at pH 7.0, temperature not specified in the publication Marasmius rotula
1.11.2.1 160
-
H2O2 at pH 7.0, temperature not specified in the publication Cyclocybe aegerita
1.11.2.1 167
-
H2O2 at pH 7.0, temperature not specified in the publication Coprinopsis cinerea