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Literature summary extracted from

  • Hupert-Kocurek, K.; Wojcieszylska, D.; Guzik, U.; Borowski, T.
    A single amino acid substitution within catalytically non-active N-terminal domain of catechol 2,3-dioxygenase (C23O) increases enzyme activity towards 4-chlorocatechol (2015), J. Mol. Catal. B, 122, 64-71 .
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.2 expressed in Escherichia coli BL21(DE3) cells Planococcus sp. S5

Protein Variants

EC Number Protein Variants Comment Organism
1.13.11.2 M65T the mutant show increased activity compared to the wild type enzyme Planococcus sp. S5

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.2 catechol + O2 Planococcus sp. S5
-
 2-hydroxymuconate-6-semialdehyde
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.2 Planococcus sp. S5 E7DDG2
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.2 0.00125
-
 wild type enzyme, with 3-methylcatechol as substrate, at pH 7.0 and 35°C Planococcus sp. S5
1.13.11.2 0.00155
-
 mutant enzyme M65T, with 3-methylcatechol as substrate, at pH 7.0 and 35°C Planococcus sp. S5
1.13.11.2 0.00938
-
 wild type enzyme, with 4-chlorocatechol as substrate, at pH 7.0 and 35°C Planococcus sp. S5
1.13.11.2 0.02065
-
 wild type enzyme, with catechol as substrate, at pH 7.0 and 35°C Planococcus sp. S5
1.13.11.2 0.0214
-
 wild type enzyme, with 4-methylcatechol as substrate, at pH 7.0 and 35°C Planococcus sp. S5
1.13.11.2 0.02245
-
 mutant enzyme M65T, with catechol as substrate, at pH 7.0 and 35°C Planococcus sp. S5
1.13.11.2 0.0312
-
 mutant enzyme M65T, with 4-methylcatechol as substrate, at pH 7.0 and 35°C Planococcus sp. S5
1.13.11.2 0.04843
-
 mutant enzyme M65T, with 4-chlorocatechol as substrate, at pH 7.0 and 35°C Planococcus sp. S5

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.2 3-methylcatechol + O2 6.12% activity compared to catechol Planococcus sp. S5 2-hydroxy-6-oxohepta-2,4-dienoate
-
 ?
1.13.11.2 4-chlorocatechol + O2 44.88% activity compared to catechol Planococcus sp. S5 4-chloro-2-hydroxymuconate semialdehyde
-
 ?
1.13.11.2 4-methylcatechol + O2 101.91% activity compared to catechol Planococcus sp. S5 2-hydroxy-3-methyl-6-oxohexa-2,4-dienoate
-
 ?
1.13.11.2 catechol + O2
-
 Planococcus sp. S5 2-hydroxymuconate-6-semialdehyde
-
 ?
1.13.11.2 catechol + O2 100% activity Planococcus sp. S5 2-hydroxymuconate-6-semialdehyde
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.2 C23o
-
 Planococcus sp. S5

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.2 35
-
-
 Planococcus sp. S5

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.2 7
-
-
 Planococcus sp. S5