EC Number | Application | Comment | Organism |
---|---|---|---|
3.4.23.21 | food industry | the peptidase may function as an important alternative enzyme in milk clotting during the preparation of cheese | Rhizomucor miehei |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.23.21 | Al3+ | 16% residual activity at 10 mM | Rhizomucor miehei | |
3.4.23.21 | Ba2+ | about 64 residual activity at 10 mM | Rhizomucor miehei | |
3.4.23.21 | Ca2+ | about 44% residual activity at 10 mM | Rhizomucor miehei | |
3.4.23.21 | Co2+ | 0.3% residual activity at 10 mM; about 36% residual activity at 10 mM | Rhizomucor miehei | |
3.4.23.21 | CTAB | in the presence of CTAB, 20 and 10 % relative activities are maintained at concentrations of 0.1% (w/v) and 1% (w/v), respectively | Rhizomucor miehei | |
3.4.23.21 | dithiothreitol | the peptidase shows residual activities of 50 and 40% following incubation with 100 or 150 mM dithiothreitol, respectively | Rhizomucor miehei | |
3.4.23.21 | guanidine | the enzyme maintains approximately 70 and 60% of proteolytic activity at 100 and 150 mM guanidine, respectively | Rhizomucor miehei | |
3.4.23.21 | K+ | about 56% residual activity at 10 mM | Rhizomucor miehei | |
3.4.23.21 | Mg2+ | about 36% residual activity at 10 mM | Rhizomucor miehei | |
3.4.23.21 | Mn2+ | about 36% residual activity at 10 mM | Rhizomucor miehei | |
3.4.23.21 | additional information | not inhibited by urea | Rhizomucor miehei | |
3.4.23.21 | pepstatin A | 40% residual activity at 0.2 mM | Rhizomucor miehei | |
3.4.23.21 | SDS | the peptidase maintains approximately 50 % of activity in the presence of 0.02 % (w/v) SDS andloses nearly all activity during incubation with 0.08 % (w/v) SDS | Rhizomucor miehei | |
3.4.23.21 | Triton X-100 | approximately 60% residual activity at 0.2 % (v/v) Triton X-100 | Rhizomucor miehei | |
3.4.23.21 | Tween 20 | approximately 60% residual activity at 0.2 % (v/v) Tween 20 | Rhizomucor miehei | |
3.4.23.23 | dithiothreitol | 100 mM, 50% residual activity | Rhizomucor miehei | |
3.4.23.23 | pepstatin A | 0.2 mM, 40% residual activity | Rhizomucor miehei | |
3.4.23.23 | SDS | 0.02%, 50% residual activity | Rhizomucor miehei | |
3.4.23.23 | Triton X-100 | 0.2%, 60% residual activity | Rhizomucor miehei |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.23.21 | 0.036 | - |
Abz-LSFMAIQ-EDDnp | at pH 5.5 and 50°C | Rhizomucor miehei | |
3.4.23.23 | 0.000036 | - |
2-aminobenzoyl-LSFMAIQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine | pH 6.5, 45°C | Rhizomucor miehei | |
3.4.23.23 | 0.000045 | - |
2-aminobenzoyl-KLRSSMQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine | pH 6.5, 45°C | Rhizomucor miehei | |
3.4.23.23 | 0.00011 | - |
2-aminobenzoyl-KLRSWKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine | pH 6.5, 45°C | Rhizomucor miehei | |
3.4.23.23 | 0.00015 | - |
2-aminobenzoyl-KLRWSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine | pH 6.5, 45°C | Rhizomucor miehei | |
3.4.23.23 | 0.0004 | - |
2-aminobenzoyl-KLRMSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine | pH 6.5, 45°C | Rhizomucor miehei | |
3.4.23.23 | 0.0005 | - |
2-aminobenzoyl-KLRSSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine | pH 6.5, 45°C | Rhizomucor miehei | |
3.4.23.23 | 0.00085 | - |
2-aminobenzoyl-KLRSKKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine | pH 6.5, 45°C | Rhizomucor miehei |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.4.23.23 | Al3+ | 5 mM, 16% residual activity | Rhizomucor miehei | |
3.4.23.23 | Ca2+ | 5 mM, 44% residual activity | Rhizomucor miehei | |
3.4.23.23 | Co2+ | 5 mM, 36% residual activity | Rhizomucor miehei | |
3.4.23.23 | Cu2+ | 5 mM, 0.3% residual activity | Rhizomucor miehei | |
3.4.23.23 | Mn2+ | 5 mM, 36% residual activity | Rhizomucor miehei |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.23.21 | Rhizomucor miehei | - |
- |
- |
3.4.23.23 | Rhizomucor miehei | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.23.21 | Sephacryl S-100 gel filtration and Sephadex G-50 resin gel filtration | Rhizomucor miehei |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.4.23.23 | 1057 | - |
pH 6.5, 45°C | Rhizomucor miehei |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.23.21 | Abz-LSFMAIQ-EDDnp + H2O | - |
Rhizomucor miehei | Abz-LSF + MAIQ-EDDnp | - |
? | |
3.4.23.21 | casein + H2O | - |
Rhizomucor miehei | ? | - |
? | |
3.4.23.21 | milk powder + H2O | - |
Rhizomucor miehei | ? | - |
? | |
3.4.23.21 | additional information | the coagulant activity of the peptidase is higher than the proteolytic activity and there is a preference for aromatic, basic, and nonpolar amino acids, particularly methionine, with specific cleavage of the peptide bond between phenylalanine and methionine | Rhizomucor miehei | ? | - |
? | |
3.4.23.23 | 2-aminobenzoyl-KLRMSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine + H2O | - |
Rhizomucor miehei | 2-aminobenzoyl-KLR + MSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine | - |
? | |
3.4.23.23 | 2-aminobenzoyl-KLRSKKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine + H2O | - |
Rhizomucor miehei | 2-aminobenzoyl-KLR + SKKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine | - |
? | |
3.4.23.23 | 2-aminobenzoyl-KLRSSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine + H2O | - |
Rhizomucor miehei | 2-aminobenzoyl-KLR + SSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine | - |
? | |
3.4.23.23 | 2-aminobenzoyl-KLRSSMQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine + H2O | - |
Rhizomucor miehei | 2-aminobenzoyl-KLR + SSMQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine | - |
? | |
3.4.23.23 | 2-aminobenzoyl-KLRSWKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine + H2O | - |
Rhizomucor miehei | 2-aminobenzoyl-KLR + SWKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine | - |
? | |
3.4.23.23 | 2-aminobenzoyl-KLRWSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine + H2O | - |
Rhizomucor miehei | 2-aminobenzoyl-KLR + WSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine | - |
? | |
3.4.23.23 | 2-aminobenzoyl-LSFMAIQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine + H2O | substrate derived from peptide sequence of kappa-casein | Rhizomucor miehei | 2-aminobenzoyl-LSF + MAIQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine | - |
? | |
3.4.23.23 | additional information | enzyme shows a preference for aromatic, basic, and nonpolar amino acids, particularly methionine, with specific cleavage of the peptide bond between phenylalanine and methionine | Rhizomucor miehei | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.4.23.21 | ? | x * 37000, SDS-PAGE | Rhizomucor miehei |
3.4.23.21 | ? | x * 37102, calculated from amino acid sequence | Rhizomucor miehei |
3.4.23.23 | ? | x * 37000, SDS-PAGE | Rhizomucor miehei |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.4.23.21 | aspartic peptidase | - |
Rhizomucor miehei |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.23.21 | 55 | - |
- |
Rhizomucor miehei |
3.4.23.23 | 55 | - |
- |
Rhizomucor miehei |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.23.21 | 40 | 65 | the enzyme maintains around 80% activity at 40-50°C. 90 and 80% proteolytic activities are maintained at 60 and 65°C, respectively | Rhizomucor miehei |
3.4.23.23 | 60 | - |
90% of maximum activity | Rhizomucor miehei |
3.4.23.23 | 65 | - |
80% of maximum activity | Rhizomucor miehei |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.23.21 | 45 | - |
the peptidase is stable at temperatures of up 45°C for 1 h | Rhizomucor miehei |
3.4.23.23 | 45 | - |
stable a up to, for 60 min | Rhizomucor miehei |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.23.23 | 0.045 | - |
2-aminobenzoyl-KLRSSMQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine | pH 6.5, 45°C | Rhizomucor miehei | |
3.4.23.23 | 0.12 | - |
2-aminobenzoyl-KLRSWKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine | pH 6.5, 45°C | Rhizomucor miehei | |
3.4.23.23 | 0.15 | - |
2-aminobenzoyl-KLRWSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine | pH 6.5, 45°C | Rhizomucor miehei | |
3.4.23.23 | 0.35 | - |
2-aminobenzoyl-KLRSSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine | pH 6.5, 45°C | Rhizomucor miehei | |
3.4.23.23 | 0.5 | - |
2-aminobenzoyl-KLRMSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine | pH 6.5, 45°C | Rhizomucor miehei | |
3.4.23.23 | 1.1 | - |
2-aminobenzoyl-KLRSKKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine | pH 6.5, 45°C | Rhizomucor miehei |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.4.23.21 | 5.5 | - |
- |
Rhizomucor miehei |
3.4.23.23 | 5.5 | - |
- |
Rhizomucor miehei |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.4.23.21 | 4.5 | 6 | more than 80% activity between pH 4.5 and 6.0 | Rhizomucor miehei |
3.4.23.23 | 5 | - |
80% of maximum activity | Rhizomucor miehei |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.4.23.21 | 3 | 5 | the peptidase is stable at pH values ranging from 3.0 to 5.0 for 1 h | Rhizomucor miehei |
3.4.23.23 | 3 | 5 | - |
Rhizomucor miehei |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.23.21 | 4722 | - |
Abz-LSFMAIQ-EDDnp | at pH 5.5 and 50°C | Rhizomucor miehei | |
3.4.23.23 | 700 | - |
2-aminobenzoyl-KLRSSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine | pH 6.5, 45°C | Rhizomucor miehei | |
3.4.23.23 | 1000 | - |
2-aminobenzoyl-KLRWSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine | pH 6.5, 45°C | Rhizomucor miehei | |
3.4.23.23 | 1000 | - |
2-aminobenzoyl-KLRSSMQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine | pH 6.5, 45°C | Rhizomucor miehei | |
3.4.23.23 | 1091 | - |
2-aminobenzoyl-KLRSWKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine | pH 6.5, 45°C | Rhizomucor miehei | |
3.4.23.23 | 1250 | - |
2-aminobenzoyl-KLRMSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine | pH 6.5, 45°C | Rhizomucor miehei | |
3.4.23.23 | 1294 | - |
2-aminobenzoyl-KLRSKKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine | pH 6.5, 45°C | Rhizomucor miehei | |
3.4.23.23 | 4722 | - |
2-aminobenzoyl-LSFMAIQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine | pH 6.5, 45°C | Rhizomucor miehei |