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Literature summary extracted from

  • Bagewadi, Z.; Mulla, S.; Ninnekar, H.
    Purification and immobilization of laccase from Trichoderma harzianum strain HZN10 and its application in dye decolorization (2017), J. Genet. Eng. Biotechnol., 15, 139-150 .
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.10.3.2 glycerol 20% (v/v), 15% increase inactivity Trichoderma harzianum
1.10.3.2 Tween-40 0.5% (v/v), 10% increase in activity Trichoderma harzianum

Application

EC Number Application Comment Organism
1.10.3.2 degradation laccase can be efficiently used to decolorize synthetic dye and help in waste water treatment Trichoderma harzianum
1.10.3.2 environmental protection laccase can be efficiently used to decolorize synthetic dye and is a suitable candidate for the treatment of wastewater from industrial effluents. The wide pH- and thermostability attributes of immobilized laccase make them suitable for environmental applications Trichoderma harzianum

General Stability

EC Number General Stability Organism
1.10.3.2 Ca-alginate, Cu-alginate, Ca-alginate-chitosan and solĀ–gel immobilized laccase show an operational stability of 36%, 51%, 66% and 82%, respectively after 6 cycles Trichoderma harzianum
1.10.3.2 soluble enzyme loses 45% of its activity after 8 days at 4Ā°C. Sol-gel matrix immobilized laccase is the most stable with a mere loss of 2% among the Ca-alginate-chitosan, Cu-alginate and Ca-alginate beads with a loss of 10%, 17% and 25%, respectively Trichoderma harzianum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.2 2-mercaptoethanol 5 mM, 15% loss of activity Trichoderma harzianum
1.10.3.2 acetone 20% (v/v), 42% loss of activity Trichoderma harzianum
1.10.3.2 Al3+ 5 mM, 60% loss of activity Trichoderma harzianum
1.10.3.2 Cd2+ 5 mM, 78% loss of activity Trichoderma harzianum
1.10.3.2 Co2+ 5 mM, 67% loss of activity Trichoderma harzianum
1.10.3.2 dithiothreitol 5 mM, 65% loss of activity Trichoderma harzianum
1.10.3.2 EDTA 5 mM, 70% loss of activity Trichoderma harzianum
1.10.3.2 ethanol 20% (v/v), 15% loss of activity Trichoderma harzianum
1.10.3.2 Fe2+ 5 mM, 60% loss of activity Trichoderma harzianum
1.10.3.2 Hg2+ 5 mM, 90% loss of activity Trichoderma harzianum
1.10.3.2 Isopropanol 20% (v/v), 30% loss of activity Trichoderma harzianum
1.10.3.2 L-cysteine 5 mM, 20% loss of activity Trichoderma harzianum
1.10.3.2 methanol 20% (v/v), 20% loss of activity Trichoderma harzianum
1.10.3.2 additional information soluble laccase shows more than 80% relative activity in organic solvents like ethanol, methanol, toluene and acetonitrile Trichoderma harzianum
1.10.3.2 Ni2+ 5 mM, 35% loss of activity Trichoderma harzianum
1.10.3.2 SDS 0.5%, 60% loss of activity Trichoderma harzianum
1.10.3.2 Toluene 20% (v/v), 18% loss of activity Trichoderma harzianum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.2 0.5
-
 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) pH 6, 25Ā°C, soluble enzyme Trichoderma harzianum
1.10.3.2 2
-
 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) pH 6, 25Ā°C,laccase immobilized in solĀ–gel matrix Trichoderma harzianum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.10.3.2 extracellular
-
 Trichoderma harzianum
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.10.3.2 Ca2+ 5 mM, 18% increase in activity Trichoderma harzianum
1.10.3.2 Cu2+ 5 mM, 85% increase in activity Trichoderma harzianum
1.10.3.2 Mg2+ 5 mM, 15% increase in activity Trichoderma harzianum
1.10.3.2 Mn2+ 5 mM, 8% increase in activity Trichoderma harzianum

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Trichoderma harzianum
-
-
-
1.10.3.2 Trichoderma harzianum HZN10
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.2
-
 Trichoderma harzianum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [Āµmol/min/mg] Specific Activity Maximum [Āµmol/min/mg] Comment Organism
1.10.3.2 164
-
 pH 6, 25Ā°C Trichoderma harzianum

Storage Stability

EC Number Storage Stability Organism
1.10.3.2 4Ā°C, 8 days, soluble enzyme loses 45% of its activity. SolĀ–gel matrix immobilized laccase is the most stable with a mere loss of 2% among the Ca-alginate-chitosan, Cu-alginate and Ca-alginate beads with a loss of 10%, 17% and 25%, respectively Trichoderma harzianum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Trichoderma harzianum ?
-
 ?
1.10.3.2 2,6-dimethoxyphenol + O2 96% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Trichoderma harzianum ?
-
 ?
1.10.3.2 catechol + O2 44% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Trichoderma harzianum ?
-
 ?
1.10.3.2 catechol + O2 44% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Trichoderma harzianum HZN10 ?
-
 ?
1.10.3.2 ferulic acid + O2 24% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Trichoderma harzianum ?
-
 ?
1.10.3.2 ferulic acid + O2 24% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Trichoderma harzianum HZN10 ?
-
 ?
1.10.3.2 gallic acid + O2 54% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Trichoderma harzianum ?
-
 ?
1.10.3.2 gallic acid + O2 54% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Trichoderma harzianum HZN10 ?
-
 ?
1.10.3.2 guaiacol + O2 83% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Trichoderma harzianum ?
-
 ?
1.10.3.2 additional information free and immobilized laccase is employed for decolorization of three different synthetic dyes (malachite green, methylene blue and congo red). Approximately 100% of malachite green, 90% of methylene blue and 60% of congo red dyes at initial concentration of 200 mg/l are decolorized within 16, 18 and 20 h, respectively by laccase immobilized in sol-gel matrix in the presence of 1-hydroxybenzotriazole mediator Trichoderma harzianum ?
-
 ?
1.10.3.2 additional information free and immobilized laccase is employed for decolorization of three different synthetic dyes (malachite green, methylene blue and congo red). Approximately 100% of malachite green, 90% of methylene blue and 60% of congo red dyes at initial concentration of 200 mg/l are decolorized within 16, 18 and 20 h, respectively by laccase immobilized in sol-gel matrix in the presence of 1-hydroxybenzotriazole mediator Trichoderma harzianum HZN10 ?
-
 ?
1.10.3.2 syringaldazine + O2 148% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Trichoderma harzianum ?
-
 ?
1.10.3.2 syringaldazine + O2 148% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Trichoderma harzianum HZN10 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.10.3.2 ? x * 56000, SDS-PAGE Trichoderma harzianum

Temperature Optimum [Ā°C]

EC Number Temperature Optimum [Ā°C] Temperature Optimum Maximum [Ā°C] Comment Organism
1.10.3.2 50
-
 soluble and immobilized laccases Trichoderma harzianum

Temperature Range [Ā°C]

EC Number Temperature Minimum [Ā°C] Temperature Maximum [Ā°C] Comment Organism
1.10.3.2 50 70 all the forms of immobilized laccase show more than 90% relative activity at higher temperature of 55Ā°C and 60Ā°C. Sol-gel immobilized laccase shows 97% (65Ā°C) and 94% (70Ā°C) relative activity at higher temperatures. Soluble enzyme loses maximum activity above 55Ā°C Trichoderma harzianum

Temperature Stability [Ā°C]

EC Number Temperature Stability Minimum [Ā°C] Temperature Stability Maximum [Ā°C] Comment Organism
1.10.3.2 50
-
 4 h, soluble and immobilized laccases retain more than 90% activity at 50Ā°C. Copper alginate shows better stability than calcium alginate immobilized laccase Trichoderma harzianum
1.10.3.2 55
-
 4 h, calcium alginate-chitosan immobilized laccase retains 91% activity Trichoderma harzianum
1.10.3.2 65
-
 4 h, sol-gel immobilized laccase retains 90% activity Trichoderma harzianum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.2 4 7 laccase immobilized in sol-gel matrix Trichoderma harzianum
1.10.3.2 5 6 copper alginate and calcium alginate-chitosan immobilized laccase Trichoderma harzianum
1.10.3.2 6
-
 soluble enzyme and calcium alginate immobilized enzyme Trichoderma harzianum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.10.3.2 4
-
 4 h, solĀ–gel immobilized laccase retains 98% of the activity. In comparison to soluble laccase better stability is observed in the order, solĀ–gel matrix > calcium alginateĀ–chitosan > copper alginate > calcium alginate Trichoderma harzianum
1.10.3.2 5 6 4 h, solĀ–gel immobilized laccase retains 100% of the activity. In comparison to soluble laccase better stability is observed in the order, solĀ–gel matrix > calcium alginateĀ–chitosan > copper alginate > calcium alginate Trichoderma harzianum
1.10.3.2 7
-
 4 h, solĀ–gel immobilized laccase retains 96% of the activity. In comparison to soluble laccase better stability is observed in the order, solĀ–gel matrix > calcium alginateĀ–chitosan > copper alginate > calcium alginate Trichoderma harzianum
1.10.3.2 8
-
 4 h, solĀ–gel immobilized laccase retains 92% of the activity. In comparison to soluble laccase better stability is observed in the order, solĀ–gel matrix > calcium alginateĀ–chitosan > copper alginate > calcium alginate Trichoderma harzianum