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Literature summary extracted from

  • Ademakinwa, A.; Agboola, F.
    Biochemical characterization and kinetic studies on a purified yellow laccase from newly isolated Aureobasidium pullulans NAC8 obtained from soil containing decayed plant matter (2016), J. Genet. Eng. Biotechnol., 14, 143-151 .
No PubMed abstract available

Application

EC Number Application Comment Organism
1.10.3.2 environmental protection laccase can be efficiently used to decolorize synthetic dye and help in waste water treatment Aureobasidium pullulans

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.2 2-mercaptoethanol 0.05 mM, 29% loss of activity Aureobasidium pullulans
1.10.3.2 acetone 50% (v/v), 90% inhibition Aureobasidium pullulans
1.10.3.2 Dichloromethane 50% (v/v), 80% inhibition Aureobasidium pullulans
1.10.3.2 dimethyl sulfoxide 50% (v/v), 85% inhibition Aureobasidium pullulans
1.10.3.2 EDTA 0.5 mM, 29% loss of activity Aureobasidium pullulans
1.10.3.2 ethanol 50% (v/v), 80% inhibition Aureobasidium pullulans
1.10.3.2 L-cysteine 0.05 mM, 44% loss of activity Aureobasidium pullulans
1.10.3.2 methanol 50% (v/v), 70% inhibition Aureobasidium pullulans
1.10.3.2 SDS 0.05 mM, 41% loss of activity Aureobasidium pullulans
1.10.3.2 Sodium azide 0.05 mM, 50% loss of activity Aureobasidium pullulans
1.10.3.2 thioglycolic acid 2.5 mM, 50% loss of activity Aureobasidium pullulans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.2 1.05
-
 guaiacol pH 5.0, 25°C Aureobasidium pullulans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.10.3.2 Ca2+ 25 mM, 70% inhibition Aureobasidium pullulans
1.10.3.2 Co2+ 25 mM, 61% inhibition Aureobasidium pullulans
1.10.3.2 Mg2+ 25 mM, 35% inhibition Aureobasidium pullulans
1.10.3.2 Mn2+ 25 mM, 55% inhibition Aureobasidium pullulans
1.10.3.2 Ni2+ 25 mM, 75% inhibition Aureobasidium pullulans

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Aureobasidium pullulans
-
-
-
1.10.3.2 Aureobasidium pullulans NAC8
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.2
-
 Aureobasidium pullulans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 caffeic acid + O2 24.3% of the activity with guaiacol Aureobasidium pullulans ?
-
 ?
1.10.3.2 caffeic acid + O2 24.3% of the activity with guaiacol Aureobasidium pullulans NAC8 ?
-
 ?
1.10.3.2 catechol + O2 85.4% of the activity with guaiacol Aureobasidium pullulans ?
-
 ?
1.10.3.2 catechol + O2 85.4% of the activity with guaiacol Aureobasidium pullulans NAC8 ?
-
 ?
1.10.3.2 gallic acid + O2 13.7% of the activity with guaiacol Aureobasidium pullulans ?
-
 ?
1.10.3.2 gallic acid + O2 13.7% of the activity with guaiacol Aureobasidium pullulans NAC8 ?
-
 ?
1.10.3.2 guaiacol + O2
-
 Aureobasidium pullulans ?
-
 ?
1.10.3.2 guaiacol + O2
-
 Aureobasidium pullulans NAC8 ?
-
 ?
1.10.3.2 tannic acid + O2 63.1 % of the activity with guaiacol Aureobasidium pullulans ?
-
 ?
1.10.3.2 tannic acid + O2 63.1 % of the activity with guaiacol Aureobasidium pullulans NAC8 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.10.3.2 ? x * 68400, SDS-PAGE Aureobasidium pullulans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.2 45
-
-
 Aureobasidium pullulans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.10.3.2 45 55 2 h, the enzyme retains more than 60% of its activity Aureobasidium pullulans
1.10.3.2 65 75 2 h, the enzyme loses nearly 80% of its activity Aureobasidium pullulans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.10.3.2 2.53
-
 guaiacol pH 5.0, 25°C Aureobasidium pullulans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.2 5
-
 substrate: guaiacol Aureobasidium pullulans
1.10.3.2 6
-
 substrate: catechol Aureobasidium pullulans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.10.3.2 3 6 1 h, stable Aureobasidium pullulans

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.10.3.2 2.4
-
 guaiacol pH 5.0, 25°C Aureobasidium pullulans