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Literature summary extracted from

  • Davey, J.F.; Trudgill, P.W.
    The metabolism of trans-cyclohexan-1,2-diol by an Acinetobacter species (1977), Eur. J. Biochem., 74, 115-127.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.66 0.0053
-
 NADPH
-
 Acinetobacter sp.
1.14.13.66 0.0062
-
 2-Hydroxycyclohexan-1-one
-
 Acinetobacter sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.63 158000
-
 gel filtration Acinetobacter sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.174 trans-cyclohexane-1,2-diol + NAD+ Acinetobacter sp. metabolic pathway of trans-cyclohexan-1,2-diol to adipate 2-hydroxycyclohexan-1-one + NADH
-
 r
1.2.1.63 6-oxohexanoate + NADP+ + H2O Nocardia globerula
-
 adipate + NADPH
-
 ?
1.2.1.63 6-oxohexanoate + NADP+ + H2O Acinetobacter sp.
-
 adipate + NADPH
-
 ?
1.2.1.63 6-oxohexanoate + NADP+ + H2O Nocardia globerula CL1
-
 adipate + NADPH
-
 ?
1.14.13.66 2-Hydroxycyclohexan-1-one + NADPH + O2 Acinetobacter sp. enzyme in the oxidation pathway of trans-cyclohexan-1,2-diol to adipate, enzyme activity is induced by trans-cyclohexan-1,2-diol ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.174 Acinetobacter sp.
-
-
-
1.1.1.174 Acinetobacter sp. TD63
-
-
-
1.2.1.63 Acinetobacter sp.
-
-
-
1.2.1.63 Acinetobacter sp. NCIB 9871
-
-
-
1.2.1.63 Acinetobacter sp. TD63
-
-
-
1.2.1.63 Nocardia globerula
-
-
-
1.2.1.63 Nocardia globerula CL1
-
-
-
1.14.13.66 Acinetobacter sp.
-
 TD63
-
1.14.13.66 Acinetobacter sp. TD63
-
 TD63
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.174 partial Acinetobacter sp.
1.2.1.63 partial Acinetobacter sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.174 culture condition:cyclohexan-1,2-diol grown cell induction of enzyme formation by trans-cyclohexan-1,2-diol containing medium Acinetobacter sp.
-
1.2.1.63 cell culture
-
 Nocardia globerula
-
1.2.1.63 cell culture strain TD63 only inducible for enzyme synthesis in the presence of trans-cyclohexan-1,2-diol-containing growth media Acinetobacter sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.174 0.85
-
 cell extracts, grown on trans-cyclohexan-1,2-diol Acinetobacter sp.
1.1.1.174 26.5
-
 partially purified Acinetobacter sp.
1.2.1.63 7.7
-
-
 Acinetobacter sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.174 trans-cyclohexane-1,2-diol + NAD+ metabolic pathway of trans-cyclohexan-1,2-diol to adipate Acinetobacter sp. 2-hydroxycyclohexan-1-one + NADH
-
 r
1.2.1.63 6-oxohexanoate + NADP+ + H2O
-
 Nocardia globerula adipate + NADPH
-
 ?
1.2.1.63 6-oxohexanoate + NADP+ + H2O
-
 Acinetobacter sp. adipate + NADPH
-
 ?
1.2.1.63 6-oxohexanoate + NADP+ + H2O
-
 Nocardia globerula CL1 adipate + NADPH
-
 ?
1.14.13.66 2-hydroxycyclohexan-1-one + NADPH + O2
-
 Acinetobacter sp. 1-oxa-2-oxo-7-hydroxycycloheptane + NADP+ + H2O 1-oxa-2-oxo-7-hydroxycycloheptane spontaneously rearranges to yield 6-oxohexanoate ?
1.14.13.66 2-hydroxycyclohexan-1-one + NADPH + O2
-
 Acinetobacter sp. TD63 1-oxa-2-oxo-7-hydroxycycloheptane + NADP+ + H2O 1-oxa-2-oxo-7-hydroxycycloheptane spontaneously rearranges to yield 6-oxohexanoate ?
1.14.13.66 2-hydroxycyclohexan-1-one + NADPH + O2
-
 Acinetobacter sp. NCIB 9871 1-oxa-2-oxo-7-hydroxycycloheptane + NADP+ + H2O 1-oxa-2-oxo-7-hydroxycycloheptane spontaneously rearranges to yield 6-oxohexanoate ?
1.14.13.66 2-Hydroxycyclohexan-1-one + NADPH + O2 enzyme in the oxidation pathway of trans-cyclohexan-1,2-diol to adipate, enzyme activity is induced by trans-cyclohexan-1,2-diol Acinetobacter sp. ?
-
 ?
1.14.13.66 2-Methylcyclohexanone + NADPH + O2
-
 Acinetobacter sp. ?
-
 ?
1.14.13.66 2-Methylcyclohexanone + NADPH + O2
-
 Acinetobacter sp. TD63 ?
-
 ?
1.14.13.66 2-Methylcyclohexanone + NADPH + O2
-
 Acinetobacter sp. NCIB 9871 ?
-
 ?
1.14.13.66 3-Methylcyclohexanone + NADPH + O2
-
 Acinetobacter sp. ?
-
 ?
1.14.13.66 3-Methylcyclohexanone + NADPH + O2
-
 Acinetobacter sp. TD63 ?
-
 ?
1.14.13.66 3-Methylcyclohexanone + NADPH + O2
-
 Acinetobacter sp. NCIB 9871 ?
-
 ?
1.14.13.66 4-Methylcyclohexanone + NADPH + O2
-
 Acinetobacter sp. ?
-
 ?
1.14.13.66 4-Methylcyclohexanone + NADPH + O2
-
 Acinetobacter sp. TD63 ?
-
 ?
1.14.13.66 4-Methylcyclohexanone + NADPH + O2
-
 Acinetobacter sp. NCIB 9871 ?
-
 ?
1.14.13.66 Cyclohexanone + NADPH + O2
-
 Acinetobacter sp. ?
-
 ?
1.14.13.66 Cyclohexanone + NADPH + O2
-
 Acinetobacter sp. TD63 ?
-
 ?
1.14.13.66 Cyclohexanone + NADPH + O2
-
 Acinetobacter sp. NCIB 9871 ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.174 7
-
 reduction of 2-hydroxycyclohexan-1-one Acinetobacter sp.
1.1.1.174 9.5 10 oxidation of trans-cyclohexane-1,2-diol Acinetobacter sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.174 NAD+
-
 Acinetobacter sp.
1.2.1.63 NADP+
-
 Acinetobacter sp.
1.14.13.66 NADPH
-
 Acinetobacter sp.