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Literature summary extracted from

  • Chen, C.; Shrestha, R.; Jia, K.; Gao, P.F.; Geisbrecht, B.V.; Bossmann, S.H.; Shi, J.; Li, P.
    Characterization of dye-decolorizing peroxidase (DyP) from Thermomonospora curvata reveals unique catalytic properties of a-type DyPs (2015), J. Biol. Chem., 290, 23447-23463 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.19 expressed in Escherichia coli BL21(DE3) cells Thermomonospora curvata

Protein Variants

EC Number Protein Variants Comment Organism
1.11.1.19 D220A the catalytic efficiency of the mutant toward H2O2 drops by 22fold compared to the wild type enzyme Thermomonospora curvata
1.11.1.19 D220F the mutant shows reduced activity compared to the wild type enzyme Thermomonospora curvata
1.11.1.19 D220G the mutant shows reduced activity compared to the wild type enzyme Thermomonospora curvata
1.11.1.19 D220H the mutant shows reduced activity compared to the wild type enzyme Thermomonospora curvata
1.11.1.19 D220K the mutant shows reduced activity compared to the wild type enzyme Thermomonospora curvata
1.11.1.19 D220N the mutant shows reduced activity compared to the wild type enzyme Thermomonospora curvata
1.11.1.19 H312A inactive Thermomonospora curvata
1.11.1.19 H312C the catalytic efficiency of the mutant toward H2O2 drops by 88fold compared to the wild type enzyme Thermomonospora curvata
1.11.1.19 R327A inactive Thermomonospora curvata

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.19 0.0052
-
 Reactive Blue 19 wild type enzyme, at pH 3.0 and 22°C Thermomonospora curvata
1.11.1.19 0.015
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) wild type enzyme, at pH 3.0 and 22°C Thermomonospora curvata
1.11.1.19 0.033
-
 H2O2 with hydroquinone as cosubstrate, wild type enzyme, at pH 3.0 and 22°C Thermomonospora curvata
1.11.1.19 0.043
-
 Reactive Blue 5 wild type enzyme, at pH 3.0 and 22°C Thermomonospora curvata
1.11.1.19 0.077
-
 H2O2 with guaiacol as cosubstrate, wild type enzyme, at pH 3.0 and 22°C Thermomonospora curvata
1.11.1.19 0.084
-
 H2O2 with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) as cosubstrate, wild type enzyme, at pH 3.0 and 22°C Thermomonospora curvata
1.11.1.19 0.17
-
 Reactive Blue 4 wild type enzyme, at pH 3.0 and 22°C Thermomonospora curvata
1.11.1.19 0.28
-
 guaiacol mutant enzyme D220A, at pH 3.0 and 22°C Thermomonospora curvata
1.11.1.19 0.37
-
 guaiacol wild type enzyme, at pH 3.0 and 22°C Thermomonospora curvata
1.11.1.19 0.66
-
 guaiacol mutant enzyme H312C, at pH 3.0 and 22°C Thermomonospora curvata
1.11.1.19 2
-
 H2O2 with guaiacol as cosubstrate, mutant enzyme D220A, at pH 3.0 and 22°C Thermomonospora curvata
1.11.1.19 6.3
-
 hydroquinone wild type enzyme, at pH 3.0 and 22°C Thermomonospora curvata
1.11.1.19 11
-
 H2O2 with guaiacol as cosubstrate, mutant enzyme H312C, at pH 3.0 and 22°C Thermomonospora curvata
1.11.1.19 19
-
 catechol wild type enzyme, at pH 3.0 and 22°C Thermomonospora curvata

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.19 Thermomonospora curvata D1A807
-
-
1.11.1.19 Thermomonospora curvata DSM 43183 D1A807
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.19 Ni-NTA column chromatography Thermomonospora curvata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.19 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2 + H+
-
 Thermomonospora curvata ?
-
 ?
1.11.1.19 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2 + H+
-
 Thermomonospora curvata DSM 43183 ?
-
 ?
1.11.1.19 catechol + H2O2 + H+ low activity Thermomonospora curvata ?
-
 ?
1.11.1.19 catechol + H2O2 + H+ low activity Thermomonospora curvata DSM 43183 ?
-
 ?
1.11.1.19 guaiacol + H2O2 + H+ low activity Thermomonospora curvata ?
-
 ?
1.11.1.19 guaiacol + H2O2 + H+ low activity Thermomonospora curvata DSM 43183 ?
-
 ?
1.11.1.19 guaiacylglycerol-beta-guaiacol ether + H2O2 + H+
-
 Thermomonospora curvata ?
-
 ?
1.11.1.19 guaiacylglycerol-beta-guaiacol ether + H2O2 + H+
-
 Thermomonospora curvata DSM 43183 ?
-
 ?
1.11.1.19 hydroquinone + H2O2 + H+
-
 Thermomonospora curvata ?
-
 ?
1.11.1.19 additional information the enzyme is inactive toward 2,6-dimethoxyphenol, 2,4-dichlorophenol, veratryl alcohol, and Reactive Black 5 Thermomonospora curvata ?
-
 ?
1.11.1.19 additional information the enzyme is inactive toward 2,6-dimethoxyphenol, 2,4-dichlorophenol, veratryl alcohol, and Reactive Black 5 Thermomonospora curvata DSM 43183 ?
-
 ?
1.11.1.19 Reactive Blue 19 + H2O2 + H+
-
 Thermomonospora curvata ?
-
 ?
1.11.1.19 Reactive Blue 4 + H2O2 + H+
-
 Thermomonospora curvata ?
-
 ?
1.11.1.19 Reactive Blue 5 + H2O2 + H+
-
 Thermomonospora curvata ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.11.1.19 ? x * 42200, calculated from amino acid sequence Thermomonospora curvata

Synonyms

EC Number Synonyms Comment Organism
1.11.1.19 dye-decolorizing peroxidase
-
 Thermomonospora curvata
1.11.1.19 DyP
-
 Thermomonospora curvata

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.11.1.19 60
-
 the enzyme retains about 70% maximal activity after incubation at 60°C for 1 h Thermomonospora curvata

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.11.1.19 0.33
-
 guaiacol wild type enzyme, at pH 3.0 and 22°C Thermomonospora curvata
1.11.1.19 0.36
-
 H2O2 with guaiacol as cosubstrate, wild type enzyme, at pH 3.0 and 22°C Thermomonospora curvata
1.11.1.19 0.52
-
 guaiacol mutant enzyme H312C, at pH 3.0 and 22°C Thermomonospora curvata
1.11.1.19 0.58
-
 guaiacol mutant enzyme D220A, at pH 3.0 and 22°C Thermomonospora curvata
1.11.1.19 0.58
-
 H2O2 with guaiacol as cosubstrate, mutant enzyme H312C, at pH 3.0 and 22°C Thermomonospora curvata
1.11.1.19 0.59
-
 H2O2 with guaiacol as cosubstrate, mutant enzyme D220A, at pH 3.0 and 22°C Thermomonospora curvata
1.11.1.19 24
-
 catechol wild type enzyme, at pH 3.0 and 22°C Thermomonospora curvata
1.11.1.19 27
-
 H2O2 with hydroquinone as cosubstrate, wild type enzyme, at pH 3.0 and 22°C Thermomonospora curvata
1.11.1.19 29
-
 Reactive Blue 5 wild type enzyme, at pH 3.0 and 22°C Thermomonospora curvata
1.11.1.19 31
-
 hydroquinone wild type enzyme, at pH 3.0 and 22°C Thermomonospora curvata
1.11.1.19 41
-
 Reactive Blue 19 wild type enzyme, at pH 3.0 and 22°C Thermomonospora curvata
1.11.1.19 110
-
 Reactive Blue 4 wild type enzyme, at pH 3.0 and 22°C Thermomonospora curvata
1.11.1.19 260
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) wild type enzyme, at pH 3.0 and 22°C Thermomonospora curvata
1.11.1.19 280
-
 H2O2 with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) as cosubstrate, wild type enzyme, at pH 3.0 and 22°C Thermomonospora curvata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.19 3
-
-
 Thermomonospora curvata

Cofactor

EC Number Cofactor Comment Organism Structure
1.11.1.19 heme
-
 Thermomonospora curvata

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.11.1.19 0.053
-
 H2O2 with guaiacol as cosubstrate, mutant enzyme H312C, at pH 3.0 and 22°C Thermomonospora curvata
1.11.1.19 0.21
-
 H2O2 with guaiacol as cosubstrate, mutant enzyme D220A, at pH 3.0 and 22°C Thermomonospora curvata
1.11.1.19 0.79
-
 guaiacol mutant enzyme H312C, at pH 3.0 and 22°C Thermomonospora curvata
1.11.1.19 0.88
-
 guaiacol wild type enzyme, at pH 3.0 and 22°C Thermomonospora curvata
1.11.1.19 1.5
-
 catechol wild type enzyme, at pH 3.0 and 22°C Thermomonospora curvata
1.11.1.19 2.1
-
 guaiacol mutant enzyme D220A, at pH 3.0 and 22°C Thermomonospora curvata
1.11.1.19 4.7
-
 H2O2 with guaiacol as cosubstrate, wild type enzyme, at pH 3.0 and 22°C Thermomonospora curvata
1.11.1.19 4.8
-
 hydroquinone wild type enzyme, at pH 3.0 and 22°C Thermomonospora curvata
1.11.1.19 650
-
 Reactive Blue 4 wild type enzyme, at pH 3.0 and 22°C Thermomonospora curvata
1.11.1.19 660
-
 Reactive Blue 5 wild type enzyme, at pH 3.0 and 22°C Thermomonospora curvata
1.11.1.19 820
-
 H2O2 with hydroquinone as cosubstrate, wild type enzyme, at pH 3.0 and 22°C Thermomonospora curvata
1.11.1.19 3400
-
 H2O2 with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) as cosubstrate, wild type enzyme, at pH 3.0 and 22°C Thermomonospora curvata
1.11.1.19 7800
-
 Reactive Blue 19 wild type enzyme, at pH 3.0 and 22°C Thermomonospora curvata
1.11.1.19 17000
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) wild type enzyme, at pH 3.0 and 22°C Thermomonospora curvata