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Literature summary extracted from

  • Eppink, M.H.M.; Boeren, S.A.; Vervoort, J.; van Berkel, W.J.H.
    Purification and properties of 4-hydroxybenzoate 1-hydroxylase (decarboxylating), a novel flavin adenine dinucleotide-dependent monooxygenase from Candida parapsilosis CBS604 (1997), J. Bacteriol., 179, 6680-6687.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.13.64 3,5-Dichloro-4-hydroxybenzoate
-
 Candida parapsilosis
1.14.13.64 4-Hydroxy-3,5-dinitrobenzoate
-
 Candida parapsilosis
1.14.13.64 4-Hydroxyisophthalate
-
 Candida parapsilosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.64 0.004
-
 2,6-Difluoro-4-hydroxybenzoate
-
 Candida parapsilosis
1.14.13.64 0.005
-
 2,5-Difluoro-4-hydroxybenzoate
-
 Candida parapsilosis
1.14.13.64 0.007
-
 2,4-Dihydroxybenzoate
-
 Candida parapsilosis
1.14.13.64 0.008
-
 3-Fluoro-4-hydroxybenzoate
-
 Candida parapsilosis
1.14.13.64 0.009
-
 3,4-dihydroxybenzoate
-
 Candida parapsilosis
1.14.13.64 0.01
-
 4-hydroxybenzoate
-
 Candida parapsilosis
1.14.13.64 0.013
-
 3-amino-4-hydroxybenzoate 4-hydroxy-3-nitrobenzoate Candida parapsilosis
1.14.13.64 0.018
-
 2-Fluoro-4-hydroxybenzoate
-
 Candida parapsilosis
1.14.13.64 0.019
-
 NADH
-
 Candida parapsilosis
1.14.13.64 0.025
-
 2-Chloro-4-hydroxybenzoate
-
 Candida parapsilosis
1.14.13.64 0.03
-
 2,3,5,6-Tetrafluoro-4-hydroxybenzoate
-
 Candida parapsilosis
1.14.13.64 0.125
-
 4-hydroxy-3-methoxybenzoate
-
 Candida parapsilosis
1.14.13.64 0.169
-
 NADPH
-
 Candida parapsilosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.13.64 49000
-
 gel filtration Candida parapsilosis
1.14.13.64 52000
-
 1 * 52000, SDS-PAGE Candida parapsilosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.64 4-Hydroxybenzoate + NAD(P)H + O2 Candida parapsilosis the enzyme is induced when the yeast is grown on either 4-hydroxybenzoate, 2,3-dihydroxybenzoate, or 3,4-dihydroxybenzoate as the sole carbon source ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.64 Candida parapsilosis
-
-
-
1.14.13.64 Candida parapsilosis CBS604
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.64
-
 Candida parapsilosis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.13.64 12
-
-
 Candida parapsilosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.64 2,3,5,6-Tetrafluoro-4-hydroxybenzoate + NADH + O2
-
 Candida parapsilosis ?
-
 ?
1.14.13.64 2,3,5,6-Tetrafluoro-4-hydroxybenzoate + NADPH + O2
-
 Candida parapsilosis ?
-
 ?
1.14.13.64 2,4-Dihydroxybenzoate + NADH + O2
-
 Candida parapsilosis 1,2,4-Trihydroxybenzene + NAD+ + CO2
-
 ?
1.14.13.64 2,4-Dihydroxybenzoate + NADH + O2
-
 Candida parapsilosis CBS604 1,2,4-Trihydroxybenzene + NAD+ + CO2
-
 ?
1.14.13.64 2,4-dihydroxybenzoate + NADPH + O2
-
 Candida parapsilosis 1,2,4-trihydroxybenzene + NADP+ + CO2
-
 ?
1.14.13.64 2,5-Difluoro-4-hydroxybenzoate + NADH + O2
-
 Candida parapsilosis ?
-
 ?
1.14.13.64 2,5-Difluoro-4-hydroxybenzoate + NADPH + O2
-
 Candida parapsilosis ?
-
 ?
1.14.13.64 2,6-Difluoro-4-hydroxybenzoate + NADH + O2
-
 Candida parapsilosis ?
-
 ?
1.14.13.64 2,6-Difluoro-4-hydroxybenzoate + NADPH + O2
-
 Candida parapsilosis ?
-
 ?
1.14.13.64 2-chloro-4-hydroxybenzoate + NADH + O2
-
 Candida parapsilosis ?
-
 ?
1.14.13.64 2-chloro-4-hydroxybenzoate + NADH + O2
-
 Candida parapsilosis CBS604 ?
-
 ?
1.14.13.64 2-Chloro-4-hydroxybenzoate + NADPH + O2
-
 Candida parapsilosis ?
-
 ?
1.14.13.64 2-fluoro-4-hydroxybenzoate + NADH + O2
-
 Candida parapsilosis 2-fluoro-1,4-dihydroxybenzene + NAD+ + CO2
-
 ?
1.14.13.64 2-fluoro-4-hydroxybenzoate + NADH + O2
-
 Candida parapsilosis CBS604 2-fluoro-1,4-dihydroxybenzene + NAD+ + CO2
-
 ?
1.14.13.64 2-fluoro-4-hydroxybenzoate + NADPH + O2
-
 Candida parapsilosis 2-fluoro-1,4-dihydroxybenzene + NADP+ + CO2
-
 ?
1.14.13.64 3,4-Dihydroxybenzoate + NADH + O2
-
 Candida parapsilosis 1,2,4-Trihydroxybenzene + NAD+ + CO2
-
 ?
1.14.13.64 3,4-Dihydroxybenzoate + NADPH + O2
-
 Candida parapsilosis 1,2,4-Trihydroxybenzene + NADP+ + CO2
-
 ?
1.14.13.64 3,5-Difluoro-4-hydroxybenzoate + NADH + O2
-
 Candida parapsilosis ?
-
 ?
1.14.13.64 3,5-Difluoro-4-hydroxybenzoate + NADPH + O2
-
 Candida parapsilosis ?
-
 ?
1.14.13.64 3-Amino-4-hydroxybenzoate + NADH + O2
-
 Candida parapsilosis ?
-
 ?
1.14.13.64 3-Amino-4-hydroxybenzoate + NADPH + O2
-
 Candida parapsilosis ?
-
 ?
1.14.13.64 3-Chloro-4-hydroxybenzoate + NADH + O2
-
 Candida parapsilosis ?
-
 ?
1.14.13.64 3-Chloro-4-hydroxybenzoate + NADPH + O2
-
 Candida parapsilosis ?
-
 ?
1.14.13.64 3-Fluoro-4-hydroxybenzoate + NADH + O2
-
 Candida parapsilosis ?
-
 ?
1.14.13.64 3-Fluoro-4-hydroxybenzoate + NADPH + O2
-
 Candida parapsilosis ?
-
 ?
1.14.13.64 4-Hydroxy-3-methoxybenzoate + NADH + O2
-
 Candida parapsilosis ?
-
 ?
1.14.13.64 4-Hydroxy-3-methoxybenzoate + NADPH + O2
-
 Candida parapsilosis ?
-
 ?
1.14.13.64 4-Hydroxy-3-nitrobenzoate + NADH + O2
-
 Candida parapsilosis ?
-
 ?
1.14.13.64 4-Hydroxy-3-nitrobenzoate + NADPH + O2
-
 Candida parapsilosis ?
-
 ?
1.14.13.64 4-Hydroxybenzoate + NAD(P)H + O2 the enzyme is induced when the yeast is grown on either 4-hydroxybenzoate, 2,3-dihydroxybenzoate, or 3,4-dihydroxybenzoate as the sole carbon source Candida parapsilosis ?
-
 ?
1.14.13.64 4-Hydroxybenzoate + NADH + O2 NADH is the preferred electron donor Candida parapsilosis 1,4-Dihydroxybenzene + NAD+ + CO2
-
 ?
1.14.13.64 4-Hydroxybenzoate + NADH + O2 NADH is the preferred electron donor Candida parapsilosis CBS604 1,4-Dihydroxybenzene + NAD+ + CO2
-
 ?
1.14.13.64 4-Hydroxybenzoate + NADPH + O2 NADH is the preferred electron donor Candida parapsilosis 1,4-Dihydroxybenzene + NADP+ + CO2
-
 ?
1.14.13.64 4-Hydroxybenzoate + NADPH + O2 NADH is the preferred electron donor Candida parapsilosis CBS604 1,4-Dihydroxybenzene + NADP+ + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.13.64 monomer 1 * 52000, SDS-PAGE Candida parapsilosis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.13.64 37
-
-
 Candida parapsilosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.13.64 6.5
-
 3,5-Difluoro-4-hydroxybenzoate
-
 Candida parapsilosis
1.14.13.64 7.5
-
 2,3,5,6-Tetrafluoro-4-hydroxybenzoate
-
 Candida parapsilosis
1.14.13.64 8.3
-
 3-amino-4-hydroxybenzoate
-
 Candida parapsilosis
1.14.13.64 8.5
-
 2,6-Difluoro-4-hydroxybenzoate
-
 Candida parapsilosis
1.14.13.64 9.6
-
 2-Chloro-4-hydroxybenzoate
-
 Candida parapsilosis
1.14.13.64 10
-
 4-hydroxybenzoate
-
 Candida parapsilosis
1.14.13.64 11.8
-
 4-hydroxy-3-methoxybenzoate
-
 Candida parapsilosis
1.14.13.64 12
-
 2,5-Difluoro-4-hydroxybenzoate
-
 Candida parapsilosis
1.14.13.64 14
-
 2-Fluoro-4-hydroxybenzoate
-
 Candida parapsilosis
1.14.13.64 16.8
-
 3,4-dihydroxybenzoate
-
 Candida parapsilosis
1.14.13.64 17
-
 3-Chloro-4-hydroxybenzoate
-
 Candida parapsilosis
1.14.13.64 18.1
-
 3-Fluoro-4-hydroxybenzoate
-
 Candida parapsilosis
1.14.13.64 19.8
-
 2,4-Dihydroxybenzoate
-
 Candida parapsilosis
1.14.13.64 19.8
-
 4-Hydroxy-3-nitrobenzoate
-
 Candida parapsilosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.13.64 8
-
-
 Candida parapsilosis

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.64 FAD contains FAD as weakly bound cofactor Candida parapsilosis
1.14.13.64 FAD Km: 0.0015 mM Candida parapsilosis