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Literature summary extracted from

  • Yang, S.; Fu, X.; Yan, Q.; Jiang, Z.; Wang, J.
    Biochemical characterization of a novel acidic exochitinase from Rhizomucor miehei with antifungal activity (2016), J. Agric. Food Chem., 64, 461-469 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.14 expression in Escherichia coli Rhizomucor miehei

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.14 Ag+ 1 mM, 66% of initial activity Rhizomucor miehei
3.2.1.14 Co2+ 1 mM, 80% of initial activity Rhizomucor miehei
3.2.1.14 Cu2+ 1 mM, 76% of initial activity Rhizomucor miehei
3.2.1.14 Hg2+ 1 mM, 34% of initial activity Rhizomucor miehei
3.2.1.14 Mg2+ 1 mM, 86% of initial activity Rhizomucor miehei
3.2.1.14 Mn2+ 1 mM, 74% of initial activity Rhizomucor miehei
3.2.1.14 Sn2+ 1 mM, 54% of initial activity Rhizomucor miehei
3.2.1.14 Zn2+ 1 mM, 85% of initial activity Rhizomucor miehei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.14 additional information
-
 glycol chitin Km value 1.55 mg/mlpH 4.5, 50°C Rhizomucor miehei
3.2.1.14 additional information
-
 colloidal chitin Km value 4.02 mg/ml, pH 4.5, 50°C Rhizomucor miehei

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.14 soluble
-
 Rhizomucor miehei
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.14 Ca2+ 1 mM, 165% of initial activity Rhizomucor miehei
3.2.1.14 Ni2+ 1 mM, 130% of initial activity Rhizomucor miehei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.14 44700
-
 gel filtration Rhizomucor miehei

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.14 Rhizomucor miehei A0A286JZ72
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.14 2.7
-
 substrate tri-N-acetyl-chitotriose, pH 4.5, 50°C Rhizomucor miehei
3.2.1.14 11.9
-
 substrate colloidal chitin, pH 4.5, 50°C Rhizomucor miehei
3.2.1.14 18.1
-
 substrate glycol chitin, pH 4.5, 50°C Rhizomucor miehei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.14 colloidal chitin + H2O
-
 Rhizomucor miehei N,N'-diacetylchitobiose + ?
-
 ?
3.2.1.14 glycol chitin + H2O
-
 Rhizomucor miehei ?
-
 ?
3.2.1.14 N,N',N''-triacetylchitotriose + 2 H2O
-
 Rhizomucor miehei 3 beta-N-acetyl-D-glucosamine
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.14 monomer 1 * 44600, SDS-PAGE, 1 * 44709, calculated Rhizomucor miehei

Synonyms

EC Number Synonyms Comment Organism
3.2.1.14 Chi44
-
 Rhizomucor miehei

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.14 50
-
-
 Rhizomucor miehei

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.14 45
-
 half-life 1209 min Rhizomucor miehei
3.2.1.14 50
-
 half-life 324 min Rhizomucor miehei
3.2.1.14 55
-
 half-life 62 min Rhizomucor miehei
3.2.1.14 60
-
 half-life 38 min Rhizomucor miehei

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.14 0.009
-
 colloidal chitin pH 4.5, 50°C Rhizomucor miehei
3.2.1.14 0.016
-
 glycol chitin pH 4.5, 50°C Rhizomucor miehei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.14 4.5
-
-
 Rhizomucor miehei

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.14 4.5 9
-
 Rhizomucor miehei

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.14 Rhizomucor miehei calculated
-
 5.3

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.14 additional information
-
 colloidal chitin kcat/Km value 0.002 ml/mg/s, pH 4.5, 50°C Rhizomucor miehei
3.2.1.14 additional information
-
 glycol chitin kcat/Km value 0.010 ml/mg/s, pH 4.5, 50°C Rhizomucor miehei