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Literature summary extracted from

  • Chen, Z.; Liu, Y.; Yan, Q.; Yang, S.; Jiang, Z.
    Biochemical characterization of a novel endo-1,5-alpha-L-arabinanase from Rhizomucor miehei (2015), J. Agric. Food Chem., 63, 1226-1233 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.99 2-mercaptoethanol 1 mM, 110% of initial activity Rhizomucor miehei
3.2.1.99 SDS 1 mM, 112% of initial activity Rhizomucor miehei

Application

EC Number Application Comment Organism
3.2.1.99 degradation synergistic action of arase and pectinase can significantly improve the degradation of sugar beet pulp Rhizomucor miehei

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.99 expression in Escherichia coli Rhizomucor miehei

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.99 Ag+ 1 mM, 34% residual activity Rhizomucor miehei
3.2.1.99 Cr2+ 1 mM, 12% residual activity Rhizomucor miehei
3.2.1.99 EDTA 1 mM, 62% residual activity Rhizomucor miehei
3.2.1.99 Fe3+ 1 mM, 18% residual activity Rhizomucor miehei
3.2.1.99 Hg2+ 1 mM, 8% residual activity Rhizomucor miehei
3.2.1.99 Sn2+ 1 mM, 30% residual activity Rhizomucor miehei
3.2.1.99 Zn2+ 1 mM, 34% residual activity Rhizomucor miehei

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.99 Co2+ 1 mM, 108% of initial activity Rhizomucor miehei
3.2.1.99 Na+ 1 mM, 113% of initial activity Rhizomucor miehei

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.99 Rhizomucor miehei A0A0C4JZB0
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.99 109
-
 substrate debranched arabinan, pH 5.5, 45°C Rhizomucor miehei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.99 debranched (1->5)-L-arabinan + H2O
-
 Rhizomucor miehei L-arabinose + L-arabinosyl-alpha-(1->5)-L-arabinoside + ?
-
 ?
3.2.1.99 additional information no substrates: birchwood xylan, 4-nitrophenyl-alpha-L-arabinofuranoside, 4-nitrophenyl-alpha-L-arabinopyranoside, and 4-nitrophenyl-beta-D-xylopyranoside Rhizomucor miehei ?
-
 ?
3.2.1.99 sugar beet arabinan + H2O 48% of the activity with debranched (1->5)-L-arabinan Rhizomucor miehei L-arabinose + L-arabinosyl-alpha-(1->5)-L-arabinoside
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.99 ? x * 40600, SDS-PAGE, x * 33500, calculated Rhizomucor miehei

Synonyms

EC Number Synonyms Comment Organism
3.2.1.99 arase
-
 Rhizomucor miehei

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.99 55
-
-
 Rhizomucor miehei

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.99 45
-
 pH 5.0-8.5, stable up to Rhizomucor miehei
3.2.1.99 50
-
 30 min, 70% residual activity Rhizomucor miehei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.99 5.5
-
-
 Rhizomucor miehei

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.99 5 8.5
-
 Rhizomucor miehei

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.99 Rhizomucor miehei calculated
-
 4.3