EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.10.3.2 | 2-mercaptoethanol | 1 mM, 23% inhibition | Sporothrix carnis | |
1.10.3.2 | Ba2+ | 1 mM, 39% inhibition | Sporothrix carnis | |
1.10.3.2 | Ca2+ | 1 mM, 37% inhibition | Sporothrix carnis | |
1.10.3.2 | Cu2+ | 1 mM, 48% inhibition | Sporothrix carnis | |
1.10.3.2 | EDTA | 1 mM, 29% inhibition | Sporothrix carnis | |
1.10.3.2 | Hg2+ | 1 mM, 46% inhibition | Sporothrix carnis | |
1.10.3.2 | Mg2+ | 1 mM, 45% inhibition | Sporothrix carnis | |
1.10.3.2 | Mn2+ | 1 mM, 45% inhibition | Sporothrix carnis | |
1.10.3.2 | SDS | 1 mM, 27% inhibition | Sporothrix carnis | |
1.10.3.2 | Sodium azide | 1 mM, 27% inhibition | Sporothrix carnis | |
1.10.3.2 | Triton X-100 | 1 mM, 35% inhibition | Sporothrix carnis | |
1.10.3.2 | Urea | 1 mM, 21% inhibition | Sporothrix carnis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.10.3.2 | 0.0316 | - |
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | pH 7.5, 30°C | Sporothrix carnis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.10.3.2 | Cu2+ | 10 mM, 1.3fold activation | Sporothrix carnis | |
1.10.3.2 | Mn2+ | 10 mM, 2.5fold activation | Sporothrix carnis |
EC Number | Organic Solvent | Comment | Organism |
---|---|---|---|
1.10.3.2 | Acetone | 50% (v/v), preincubation at 30°C for 30 min before addition of substrate, 27% loss of activity | Sporothrix carnis |
1.10.3.2 | acetonitrile | 50% (v/v), preincubation at 30°C for 30 min before addition of substrate, 39% loss of activity | Sporothrix carnis |
1.10.3.2 | dimethyl sulfoxide | 50% (v/v), preincubation at 30°C for 30 min before addition of substrate, 46% loss of activity | Sporothrix carnis |
1.10.3.2 | dimethylformamide | 50% (v/v), preincubation at 30°C for 30 min before addition of substrate, 36% loss of activity | Sporothrix carnis |
1.10.3.2 | Ethanol | 50% (v/v), preincubation at 30°C for 30 min before addition of substrate, 34% loss of activity | Sporothrix carnis |
1.10.3.2 | Methanol | 50% (v/v), preincubation at 30°C for 30 min before addition of substrate, 29% loss of activity | Sporothrix carnis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.10.3.2 | Sporothrix carnis | - |
- |
- |
1.10.3.2 | Sporothrix carnis CPF-05 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.10.3.2 | - |
Sporothrix carnis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.10.3.2 | 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2 | - |
Sporothrix carnis | ? | - |
? | |
1.10.3.2 | 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2 | - |
Sporothrix carnis CPF-05 | ? | - |
? | |
1.10.3.2 | 4-methylcatechol + O2 | 98% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Sporothrix carnis | ? | - |
? | |
1.10.3.2 | 4-methylcatechol + O2 | 98% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Sporothrix carnis CPF-05 | ? | - |
? | |
1.10.3.2 | guaiacol + O2 | 98% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Sporothrix carnis | ? | - |
? | |
1.10.3.2 | guaiacol + O2 | 98% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Sporothrix carnis CPF-05 | ? | - |
? | |
1.10.3.2 | pyrocatechol + O2 | 99% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Sporothrix carnis | ? | - |
? | |
1.10.3.2 | pyrocatechol + O2 | 99% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Sporothrix carnis CPF-05 | ? | - |
? | |
1.10.3.2 | syringaldazine + O2 | 90% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Sporothrix carnis | ? | - |
? | |
1.10.3.2 | syringaldazine + O2 | 90% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Sporothrix carnis CPF-05 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.10.3.2 | ? | x * 56000, SDS-PAGE | Sporothrix carnis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.10.3.2 | 50 | - |
substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Sporothrix carnis |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.10.3.2 | 30 | 90 | 30°C: 95% of maximal activity, 90°C: 50% of maximal activity | Sporothrix carnis |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.10.3.2 | 30 | - |
the enzyme retains 94% of its initial activity after 180 min of incubation with 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) as substrate | Sporothrix carnis |
1.10.3.2 | 40 | - |
the enzyme retains 98% of its initial activity after 180 min of incubation with 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) as substrate | Sporothrix carnis |
1.10.3.2 | 60 | - |
the enzyme retains 91% of its initial activity after 180 min of incubation with 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) as substrate | Sporothrix carnis |
1.10.3.2 | 70 | - |
the enzyme retains 84% of its initial activity after 180 min of incubation with 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) as substrate | Sporothrix carnis |
1.10.3.2 | 80 | - |
the enzyme retains 65% of its initial activity after 180 min of incubation with 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) as substrate | Sporothrix carnis |
1.10.3.2 | 90 | - |
the enzyme retains 50% of its initial activity after 180 min of incubation with 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) as substrate | Sporothrix carnis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.10.3.2 | 7 | - |
substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Sporothrix carnis |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.10.3.2 | 3 | 11 | pH 3.0: 93% of maximal activity, pH 11.0: 91% of maximal activity | Sporothrix carnis |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.10.3.2 | 3 | 11 | 2 h, enzyme retains over 60% of its initial activity | Sporothrix carnis |
1.10.3.2 | 11 | - |
2 h, no loss of activity | Sporothrix carnis |