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Literature summary extracted from

  • Olajuyigbe, F.M.; Fatokun, C.O.
    Biochemical characterization of an extremely stable pH-versatile laccase from Sporothrix carnis CPF-05 (2017), Int. J. Biol. Macromol., 94, 535-543 .
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.2 2-mercaptoethanol 1 mM, 23% inhibition Sporothrix carnis
1.10.3.2 Ba2+ 1 mM, 39% inhibition Sporothrix carnis
1.10.3.2 Ca2+ 1 mM, 37% inhibition Sporothrix carnis
1.10.3.2 Cu2+ 1 mM, 48% inhibition Sporothrix carnis
1.10.3.2 EDTA 1 mM, 29% inhibition Sporothrix carnis
1.10.3.2 Hg2+ 1 mM, 46% inhibition Sporothrix carnis
1.10.3.2 Mg2+ 1 mM, 45% inhibition Sporothrix carnis
1.10.3.2 Mn2+ 1 mM, 45% inhibition Sporothrix carnis
1.10.3.2 SDS 1 mM, 27% inhibition Sporothrix carnis
1.10.3.2 Sodium azide 1 mM, 27% inhibition Sporothrix carnis
1.10.3.2 Triton X-100 1 mM, 35% inhibition Sporothrix carnis
1.10.3.2 Urea 1 mM, 21% inhibition Sporothrix carnis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.2 0.0316
-
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) pH 7.5, 30°C Sporothrix carnis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.10.3.2 Cu2+ 10 mM, 1.3fold activation Sporothrix carnis
1.10.3.2 Mn2+ 10 mM, 2.5fold activation Sporothrix carnis

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
1.10.3.2 Acetone 50% (v/v), preincubation at 30°C for 30 min before addition of substrate, 27% loss of activity Sporothrix carnis
1.10.3.2 acetonitrile 50% (v/v), preincubation at 30°C for 30 min before addition of substrate, 39% loss of activity Sporothrix carnis
1.10.3.2 dimethyl sulfoxide 50% (v/v), preincubation at 30°C for 30 min before addition of substrate, 46% loss of activity Sporothrix carnis
1.10.3.2 dimethylformamide 50% (v/v), preincubation at 30°C for 30 min before addition of substrate, 36% loss of activity Sporothrix carnis
1.10.3.2 Ethanol 50% (v/v), preincubation at 30°C for 30 min before addition of substrate, 34% loss of activity Sporothrix carnis
1.10.3.2 Methanol 50% (v/v), preincubation at 30°C for 30 min before addition of substrate, 29% loss of activity Sporothrix carnis

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Sporothrix carnis
-
-
-
1.10.3.2 Sporothrix carnis CPF-05
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.2
-
Sporothrix carnis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
Sporothrix carnis ?
-
?
1.10.3.2 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
Sporothrix carnis CPF-05 ?
-
?
1.10.3.2 4-methylcatechol + O2 98% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Sporothrix carnis ?
-
?
1.10.3.2 4-methylcatechol + O2 98% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Sporothrix carnis CPF-05 ?
-
?
1.10.3.2 guaiacol + O2 98% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Sporothrix carnis ?
-
?
1.10.3.2 guaiacol + O2 98% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Sporothrix carnis CPF-05 ?
-
?
1.10.3.2 pyrocatechol + O2 99% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Sporothrix carnis ?
-
?
1.10.3.2 pyrocatechol + O2 99% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Sporothrix carnis CPF-05 ?
-
?
1.10.3.2 syringaldazine + O2 90% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Sporothrix carnis ?
-
?
1.10.3.2 syringaldazine + O2 90% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Sporothrix carnis CPF-05 ?
-
?

Subunits

EC Number Subunits Comment Organism
1.10.3.2 ? x * 56000, SDS-PAGE Sporothrix carnis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.2 50
-
substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Sporothrix carnis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.10.3.2 30 90 30°C: 95% of maximal activity, 90°C: 50% of maximal activity Sporothrix carnis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.10.3.2 30
-
the enzyme retains 94% of its initial activity after 180 min of incubation with 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) as substrate Sporothrix carnis
1.10.3.2 40
-
the enzyme retains 98% of its initial activity after 180 min of incubation with 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) as substrate Sporothrix carnis
1.10.3.2 60
-
the enzyme retains 91% of its initial activity after 180 min of incubation with 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) as substrate Sporothrix carnis
1.10.3.2 70
-
the enzyme retains 84% of its initial activity after 180 min of incubation with 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) as substrate Sporothrix carnis
1.10.3.2 80
-
the enzyme retains 65% of its initial activity after 180 min of incubation with 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) as substrate Sporothrix carnis
1.10.3.2 90
-
the enzyme retains 50% of its initial activity after 180 min of incubation with 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) as substrate Sporothrix carnis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.2 7
-
substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Sporothrix carnis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.10.3.2 3 11 pH 3.0: 93% of maximal activity, pH 11.0: 91% of maximal activity Sporothrix carnis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.10.3.2 3 11 2 h, enzyme retains over 60% of its initial activity Sporothrix carnis
1.10.3.2 11
-
2 h, no loss of activity Sporothrix carnis