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Literature summary extracted from

  • Yao, P.; Lin, Y.; Wu, G.; Lu, Y.; Zhan, T.; Kumar, A.; Zhang, L.; Liu, Z.
    Improvement of glycine oxidase by DNA shuffling, and site-saturation mutagenesis of F247 residue (2015), Int. J. Biol. Macromol., 79, 965-970 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.3.19 expressed in Escherichia coli BL21(DE3) cells Bacillus cereus

Protein Variants

EC Number Protein Variants Comment Organism
1.4.3.19 F247P inactive Bacillus cereus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.3.19 2-iminoacetate + H2O Bacillus cereus
-
glyoxylate + NH3
-
?
1.4.3.19 glycine + H2O + O2 Bacillus cereus
-
glyoxylate + NH3 + H2O2
-
?
1.4.3.19 glycine + O2 Bacillus cereus
-
2-iminoacetate + H2O2
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.19 Bacillus cereus L7T8Y9
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.19 2-iminoacetate + H2O
-
Bacillus cereus glyoxylate + NH3
-
?
1.4.3.19 glycine + H2O + O2
-
Bacillus cereus glyoxylate + NH3 + H2O2
-
?
1.4.3.19 glycine + O2
-
Bacillus cereus 2-iminoacetate + H2O2
-
?
1.4.3.19 sarcosine + H2O + O2
-
Bacillus cereus glyoxylate + methylamine + H2O2
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.3.19 60
-
-
Bacillus cereus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.3.19 8.5
-
-
Bacillus cereus