EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.10.3.2 | dithiothreitol | activates laccase at lower concentration (0.1 mM) while inhibites the enzyme at concentrations beyond 0.1 mM in a concentration-dependent manner with complete inhibition inactivity at 10 mM | Carica papaya |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.10.3.2 | dithiothreitol | activates laccase at lower concentration (0.1 mM) while inhibits the enzyme at concentrations beyond 0.1 mM in a concentration-dependent manner with complete inhibition of activity at 10 mM | Carica papaya | |
1.10.3.2 | EDTA | - |
Carica papaya | |
1.10.3.2 | Hg2+ | concentration-dependent inhibition up to 1 mM | Carica papaya | |
1.10.3.2 | Na+ | exhibits activation of laccase up to 1 mM beyond which it exhibits inhibition of laccase activity | Carica papaya | |
1.10.3.2 | SDS | - |
Carica papaya |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.10.3.2 | 0.04 | - |
2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | pH 7.5, 37°C | Carica papaya | |
1.10.3.2 | 1.48 | - |
catechol | pH 7.5, 37°C | Carica papaya | |
1.10.3.2 | 11.33 | - |
hydroquinone | pH 7.5, 37°C | Carica papaya |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.10.3.2 | Ca2+ | activates laccase in a concentration-dependent manner (0.1-10 mM) | Carica papaya | |
1.10.3.2 | Cd2+ | activates laccase in a concentration-dependent manner (0.1-10 mM) | Carica papaya | |
1.10.3.2 | Co2+ | activates laccase in aconcentration-dependent manner up to 1 mM | Carica papaya | |
1.10.3.2 | Cu2+ | activates laccase in aconcentration-dependent manner up to 1 mM | Carica papaya | |
1.10.3.2 | Fe2+ | activates laccase in aconcentration-dependent manner up to 1 mM | Carica papaya | |
1.10.3.2 | Mn2+ | activates laccase in a concentration-dependent manner (0.1-10 mM) | Carica papaya | |
1.10.3.2 | Na+ | exhibits activation of laccase up to 1 mM beyond which it exhibited inhibition of laccase activity | Carica papaya |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.10.3.2 | 260000 | - |
gel filtration | Carica papaya |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.10.3.2 | Carica papaya | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.10.3.2 | two step procedure: heat treatment (at 70°C) and Con-A affinity chromatography | Carica papaya |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.10.3.2 | leaf | - |
Carica papaya | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.10.3.2 | 41.3 | - |
pH 7.5, 37°C | Carica papaya |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.10.3.2 | 2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2 | - |
Carica papaya | ? | - |
? | |
1.10.3.2 | 2-benzoquinone + O2 | - |
Carica papaya | ? | - |
? | |
1.10.3.2 | catechol + O2 | - |
Carica papaya | ? | - |
? | |
1.10.3.2 | hydroquinone + O2 | - |
Carica papaya | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.10.3.2 | hexamer | 2 * 52000 + 2 * 45000 + 1 * 38000 + 1 * 25000, SDS-PAGE | Carica papaya |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.10.3.2 | 70 | - |
- |
Carica papaya |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.10.3.2 | 40 | 90 | 40°C: about 60% of maximalm activity, 90°C: about 75% of maximal activity | Carica papaya |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.10.3.2 | 50 | - |
1 h, 25% loss of activity | Carica papaya |
1.10.3.2 | 60 | - |
1 h, 18% loss of activity | Carica papaya |
1.10.3.2 | 70 | - |
1 h, 47% loss of activity | Carica papaya |
1.10.3.2 | 80 | - |
1 h, complete loss of activity | Carica papaya |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.10.3.2 | 0.00403 | - |
2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | pH 7.5, 37°C | Carica papaya | |
1.10.3.2 | 0.0165 | - |
catechol | pH 7.5, 37°C | Carica papaya | |
1.10.3.2 | 0.051 | - |
hydroquinone | pH 7.5, 37°C | Carica papaya |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.10.3.2 | 6 | - |
non-phenolic substrate: 2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Carica papaya |
1.10.3.2 | 8 | - |
phenolic substrate: catechol | Carica papaya |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.10.3.2 | 7.5 | 9 | pH 7.5: about 65% of maximal activity, pH 9.0: 72% of maximal activity | Carica papaya |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.10.3.2 | 6.5 | 9 | 24 h, 37°C, the enzyme retains more than 75% of initial activity | Carica papaya |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.10.3.2 | 0.0045 | - |
hydroquinone | pH 7.5, 37°C | Carica papaya | |
1.10.3.2 | 0.011 | - |
catechol | pH 7.5, 37°C | Carica papaya | |
1.10.3.2 | 0.1 | - |
2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | pH 7.5, 37°C | Carica papaya |