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Literature summary extracted from

  • Jaiswal, N.; Pandey, V.P.; Dwivedi, U.N.
    Purification of a thermostable alkaline laccase from papaya (Carica papaya) using affinity chromatography (2015), Int. J. Biol. Macromol., 72, 326-332 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.10.3.2 dithiothreitol activates laccase at lower concentration (0.1 mM) while inhibites the enzyme at concentrations beyond 0.1 mM in a concentration-dependent manner with complete inhibition inactivity at 10 mM Carica papaya

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.2 dithiothreitol activates laccase at lower concentration (0.1 mM) while inhibits the enzyme at concentrations beyond 0.1 mM in a concentration-dependent manner with complete inhibition of activity at 10 mM Carica papaya
1.10.3.2 EDTA
-
Carica papaya
1.10.3.2 Hg2+ concentration-dependent inhibition up to 1 mM Carica papaya
1.10.3.2 Na+ exhibits activation of laccase up to 1 mM beyond which it exhibits inhibition of laccase activity Carica papaya
1.10.3.2 SDS
-
Carica papaya

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.2 0.04
-
2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) pH 7.5, 37°C Carica papaya
1.10.3.2 1.48
-
catechol pH 7.5, 37°C Carica papaya
1.10.3.2 11.33
-
hydroquinone pH 7.5, 37°C Carica papaya

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.10.3.2 Ca2+ activates laccase in a concentration-dependent manner (0.1-10 mM) Carica papaya
1.10.3.2 Cd2+ activates laccase in a concentration-dependent manner (0.1-10 mM) Carica papaya
1.10.3.2 Co2+ activates laccase in aconcentration-dependent manner up to 1 mM Carica papaya
1.10.3.2 Cu2+ activates laccase in aconcentration-dependent manner up to 1 mM Carica papaya
1.10.3.2 Fe2+ activates laccase in aconcentration-dependent manner up to 1 mM Carica papaya
1.10.3.2 Mn2+ activates laccase in a concentration-dependent manner (0.1-10 mM) Carica papaya
1.10.3.2 Na+ exhibits activation of laccase up to 1 mM beyond which it exhibited inhibition of laccase activity Carica papaya

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.10.3.2 260000
-
gel filtration Carica papaya

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Carica papaya
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.2 two step procedure: heat treatment (at 70°C) and Con-A affinity chromatography Carica papaya

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.10.3.2 leaf
-
Carica papaya
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.10.3.2 41.3
-
pH 7.5, 37°C Carica papaya

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
Carica papaya ?
-
?
1.10.3.2 2-benzoquinone + O2
-
Carica papaya ?
-
?
1.10.3.2 catechol + O2
-
Carica papaya ?
-
?
1.10.3.2 hydroquinone + O2
-
Carica papaya ?
-
?

Subunits

EC Number Subunits Comment Organism
1.10.3.2 hexamer 2 * 52000 + 2 * 45000 + 1 * 38000 + 1 * 25000, SDS-PAGE Carica papaya

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.2 70
-
-
Carica papaya

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.10.3.2 40 90 40°C: about 60% of maximalm activity, 90°C: about 75% of maximal activity Carica papaya

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.10.3.2 50
-
1 h, 25% loss of activity Carica papaya
1.10.3.2 60
-
1 h, 18% loss of activity Carica papaya
1.10.3.2 70
-
1 h, 47% loss of activity Carica papaya
1.10.3.2 80
-
1 h, complete loss of activity Carica papaya

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.10.3.2 0.00403
-
2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) pH 7.5, 37°C Carica papaya
1.10.3.2 0.0165
-
catechol pH 7.5, 37°C Carica papaya
1.10.3.2 0.051
-
hydroquinone pH 7.5, 37°C Carica papaya

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.2 6
-
non-phenolic substrate: 2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Carica papaya
1.10.3.2 8
-
phenolic substrate: catechol Carica papaya

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.10.3.2 7.5 9 pH 7.5: about 65% of maximal activity, pH 9.0: 72% of maximal activity Carica papaya

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.10.3.2 6.5 9 24 h, 37°C, the enzyme retains more than 75% of initial activity Carica papaya

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.10.3.2 0.0045
-
hydroquinone pH 7.5, 37°C Carica papaya
1.10.3.2 0.011
-
catechol pH 7.5, 37°C Carica papaya
1.10.3.2 0.1
-
2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) pH 7.5, 37°C Carica papaya