Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kwon, D.; Suma, Y.; Tiwari, M.; Choi, J.; Kang, L.; Lee, J.; Kim, H.
    Decomposition of aromatic hydrocarbon intermediates by recombinant hydroxyquinol 1,2-dioxygenase from Arthrobacter chlorophenolicus A6 and its structure characterization (2014), Int. Biodeter. Biodegrad., 95, 67-75 .
No PubMed abstract available

Application

EC Number Application Comment Organism
1.13.11.37 degradation the highly purified Ar 1,2-HQD can be used as a key enzyme in the biodegradation of aromatic hydrocarbon contaminants Pseudarthrobacter chlorophenolicus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.37 expressed in Escherichia coli BL21(DE3) cells Pseudarthrobacter chlorophenolicus
1.13.11.37 gene cphA-I, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Pseudarthrobacter chlorophenolicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.37 hydroxyquinol + O2 Pseudarthrobacter chlorophenolicus
-
maleylacetate
-
?
1.13.11.37 hydroxyquinol + O2 Pseudarthrobacter chlorophenolicus A6 / DSM 12829
-
maleylacetate
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.37 Pseudarthrobacter chlorophenolicus
-
-
-
1.13.11.37 Pseudarthrobacter chlorophenolicus Q3BEM2
-
-
1.13.11.37 Pseudarthrobacter chlorophenolicus A6 / DSM 12829
-
-
-
1.13.11.37 Pseudarthrobacter chlorophenolicus A6 / DSM 12829 Q3BEM2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.37 ammonium sulfate precipitation and Ni-NTA column chromatography Pseudarthrobacter chlorophenolicus
1.13.11.37 recombinant His-tagged enzyme by nickel affinity chromatography and gel filtration by 51.7-65.2fold from Escherichia coli strain BL21(DE3) Pseudarthrobacter chlorophenolicus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.37 5.5
-
recombinant enzyme, crude enzyme extract, substrate 4-chlorocatechol, pH 7.5, 25°C Pseudarthrobacter chlorophenolicus
1.13.11.37 15.3
-
recombinant enzyme, crude enzyme extract, substrate hydroxyquinol, pH 7.5, 25°C Pseudarthrobacter chlorophenolicus
1.13.11.37 284.6
-
51.7fold purified enzyme, with 4-chlorocatechol as substrate, at pH 7.5 and 25°C Pseudarthrobacter chlorophenolicus
1.13.11.37 284.6
-
purified recombinant enzyme, substrate 4-chlorocatechol, pH 7.5, 25°C Pseudarthrobacter chlorophenolicus
1.13.11.37 391
-
62.5fold purified enzyme, with catechol as substrate, at pH 7.5 and 25°C Pseudarthrobacter chlorophenolicus
1.13.11.37 391
-
purified recombinant enzyme, substrate catechol , pH 7.5, 25°C Pseudarthrobacter chlorophenolicus
1.13.11.37 479.5
-
purified recombinant enzyme, substrate 3-methylcatechol, pH 7.5, 25°C Pseudarthrobacter chlorophenolicus
1.13.11.37 479.6
-
65.2fold purified enzyme, with 3-methylcatechol as substrate, at pH 7.5 and 25°C Pseudarthrobacter chlorophenolicus
1.13.11.37 795.6
-
52fold purified enzyme, with hydroxyquinol as substrate, at pH 7.5 and 25°C Pseudarthrobacter chlorophenolicus
1.13.11.37 795.6
-
purified recombinant enzyme, substrate hydroxyquinol, pH 7.5, 25°C Pseudarthrobacter chlorophenolicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.37 3-methylcatechol + O2
-
Pseudarthrobacter chlorophenolicus ?
-
?
1.13.11.37 3-methylcatechol + O2
-
Pseudarthrobacter chlorophenolicus A6 / DSM 12829 ?
-
?
1.13.11.37 4-chlorocatechol + O2
-
Pseudarthrobacter chlorophenolicus ?
-
?
1.13.11.37 4-chlorocatechol + O2
-
Pseudarthrobacter chlorophenolicus A6 / DSM 12829 ?
-
?
1.13.11.37 catechol + O2
-
Pseudarthrobacter chlorophenolicus ?
-
?
1.13.11.37 catechol + O2
-
Pseudarthrobacter chlorophenolicus A6 / DSM 12829 ?
-
?
1.13.11.37 catechol + O2
-
Pseudarthrobacter chlorophenolicus cis,cis-muconate
-
?
1.13.11.37 catechol + O2
-
Pseudarthrobacter chlorophenolicus A6 / DSM 12829 cis,cis-muconate
-
?
1.13.11.37 hydroxyquinol + O2
-
Pseudarthrobacter chlorophenolicus maleylacetate
-
?
1.13.11.37 hydroxyquinol + O2
-
Pseudarthrobacter chlorophenolicus A6 / DSM 12829 maleylacetate
-
?
1.13.11.37 additional information decomposition of aromatic hydrocarbon intermediates by recombinant hydroxyquinol 1,2-dioxygenase. Substrate specificity of Ar 1,2-HQD indicates that the catalytic function of this enzyme is similar to that of the type-II catechol 1,2-dioxygenase (1,2-CTD) Pseudarthrobacter chlorophenolicus ?
-
?
1.13.11.37 additional information decomposition of aromatic hydrocarbon intermediates by recombinant hydroxyquinol 1,2-dioxygenase. Substrate specificity of Ar 1,2-HQD indicates that the catalytic function of this enzyme is similar to that of the type-II catechol 1,2-dioxygenase (1,2-CTD) Pseudarthrobacter chlorophenolicus A6 / DSM 12829 ?
-
?

Subunits

EC Number Subunits Comment Organism
1.13.11.37 ? x * 35000-37000, SDS-PAGE Pseudarthrobacter chlorophenolicus
1.13.11.37 ? x * 35000-37000, recombinant His-tagged enzyme, SDS-PAGE, x * 33000-33500, about, His-tagged enzyme, sequence calculation Pseudarthrobacter chlorophenolicus
1.13.11.37 More enzyme Ar 1,2-HQD is an intradiol dioxygenase and it is related closely to 1,2-CTD. Its structure consisted of 5 alpha-helices in the N-terminal domain and 13 beta-sheets in the C-terminal domain. Three-dimensional enzyme structure homology modeling using structure PDB ID 1TMXA as template, overview Pseudarthrobacter chlorophenolicus

Synonyms

EC Number Synonyms Comment Organism
1.13.11.37 1,2-HQD
-
Pseudarthrobacter chlorophenolicus
1.13.11.37 Ar 1,2-HQD
-
Pseudarthrobacter chlorophenolicus
1.13.11.37 cphA-I
-
Pseudarthrobacter chlorophenolicus
1.13.11.37 HQD
-
Pseudarthrobacter chlorophenolicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.37 25
-
assay at Pseudarthrobacter chlorophenolicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.37 7.5
-
assay at Pseudarthrobacter chlorophenolicus

General Information

EC Number General Information Comment Organism
1.13.11.37 additional information three-dimensional enzyme structure homology modeling using structure PDB ID 1TMXA as template, overview Pseudarthrobacter chlorophenolicus
1.13.11.37 physiological function decomposition of aromatic hydrocarbon intermediates by recombinant hydroxyquinol 1,2-dioxygenase. Substrate hydroxyquinol is an intermediate found in the central pathway of biodegradation of various aromatic compounds by yeast, fungi, and bacteria Pseudarthrobacter chlorophenolicus